Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Magnetic Fe3O4 polymer submicron sphere prepared by hydro thermal method and application thereof

A polymer, sub-micron technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of poor monodispersity and controllability, single use, and complicated preparation, and achieve good monodispersity. , uniform size effect

Inactive Publication Date: 2009-12-23
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
View PDF0 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the above research progress, it can be seen that the current multifunctional polymer magnetic nanospheres have the disadvantages of cumbersome preparation, single use, poor monodispersity and controllability, etc. Controllable preparation of magnetic materials is necessary, and it has become a difficult and hot issue that urgently needs to be solved at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Magnetic Fe3O4 polymer submicron sphere prepared by hydro thermal method and application thereof
  • Magnetic Fe3O4 polymer submicron sphere prepared by hydro thermal method and application thereof
  • Magnetic Fe3O4 polymer submicron sphere prepared by hydro thermal method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1): Controllable preparation of magnetic Fe 3 o 4 Dextran magnetic Fe coated with dextran on the surface of the ball 3 o 4 Polymer submicron spheres.

[0035] Prepare 0.1mol / L FeCl 3 ·6H 2 O to the ethylene glycol solution, add anhydrous sodium acetate to make the final concentration 0.05mol / L, and then add dextran with a molecular weight of 10000 to make the final concentration 0.05mg / ml. The above mixture was placed in a closed reaction kettle and reacted for 25 hours at 100°C to obtain a solid magnetic Fe 3 o 4 Dextran magnetic Fe coated with dextran on the surface of the ball 3 o 4 Polymer submicron spheres.

[0036] Step 2): the dextran magnetic Fe obtained in step 1) 3 o 4 Polymer submicron spheres carry FITC for multifunctionality and application in cell imaging.

[0037] The dextran magnetic Fe that 20mg step 1) obtains 3 o 4 Add polymer submicron spheres to 1 mg / ml green fluorescent dye FITC, stir at room temperature for 12 hours, and collect...

Embodiment 2

[0040] Step 1): Controllable preparation of magnetic Fe 3 o 4 Dextran Magnetic Fe 3 o 4 submicron spheres.

[0041] Prepare an ethylene glycol solution of 0.25 mol / L ferric nitrate, add anhydrous sodium acetate to make the final concentration 1 mol / L, and then add dextran with a molecular weight of 10,000 to make the final concentration 100 mg / ml. The above mixture was placed in a closed reaction kettle and reacted for 5 hours at 200 ° C to obtain a solid magnetic Fe 3 o4 Dextran magnetic Fe coated with dextran on the surface of the ball 3 o 4 Polymer submicron spheres.

[0042] Step 2): the dextran magnetic Fe obtained in step 1) 3 o 4 Polymer submicron spheres carry FITC for multifunctionality and application in cell imaging.

[0043] Add 20 mg of dextran magnetic submicron spheres obtained in step 1) to 1 mg / ml quantum dots, stir and react at room temperature for 12 hours, and collect dextran magnetic Fe with a magnet 3 o 4 Polymer submicron spheres were washed t...

Embodiment 3

[0046] Step 1): Controllable preparation of magnetic Fe 3 o 4 Spherical Chitosan-Coated Chitosan Magnetic Fe 3 o 4 submicron spheres.

[0047] Prepare 0.01mol / L FeCl 3 ·6H 2 O to the ethylene glycol solution, add anhydrous sodium acetate to make the final concentration 10mol / L, and then add chitosan with a molecular weight of 50000-800000 to make the final concentration 20mg / ml. The above mixture was placed in a closed reaction kettle and reacted at 150°C for 18 hours to obtain a solid magnetic Fe 3 o 4 Chitosan magnetic Fe coated with chitosan on the surface of the ball 3 o 4 Polymer submicron spheres.

[0048] Step 2): the chitosan magnetic Fe that step 1) obtains 3 o 4 Multifunctionalization of polymer submicron spheres coupled with FITC and colocalization with lysosomes.

[0049] Colocalization with lysosomes: chitosan magnetic Fe 3 o 4 After polymer submicron spheres are covalently coupled to FITC, take 1 mg of chitosan magnetic Fe after covalently coupling ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of controllable preparation and application of nano materials, and in particular relates to a magnetic Fe3O4 polymer submicron sphere prepared by a hydro thermal method and application thereof. Ferric salt is taken as an iron source, polymer glucan, chitosan or polyacrylic acid is taken as a surfactant, and the magnetic Fe3O4 polymer submicron sphere, namely the surface of a magnetic Fe3O4 is coated with the glucan, the chitosan or the polyacrylic acid, is prepared by the hydro thermal method at one step. According to the difference of functional groups of the polymer, the magnetic Fe3O4 polymer submicron sphere can be combined with different functional molecules to realize different application in biology. The size, uniformity and monodispersity of the three types of magnetic Fe3O4 polymer submicron spheres can be better controlled through reaction conditions, and the size of the spheres is between 200 and 1,000nm.

Description

technical field [0001] The invention belongs to the technical field of controllable preparation and application of nanomaterials, in particular to multifunctional magnetic Fe prepared by hydrothermal method. 3 o 4 Polymer submicron spheres and magnetic Fe 3 o 4 Use of polymeric submicron spheres. Background technique [0002] Due to their strong magnetism and low cytotoxicity, magnetic nanomaterials have great application potential in biomedicine, such as purification of antibodies and fusion proteins, delivery of drugs or genes, immunoassays, and magnetic resonance imaging. However, there are serious defects in the application of pure magnetic nanomaterials in biomedicine. Therefore, in recent years, the multifunctionalization of magnetic nanomaterials has become a research hotspot due to its novel ideas and wide applications. Cytotoxicity and therapeutic equal advantages in one. Inorganic materials have advantages in controllability, such as good dispersion, easy cont...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L5/02C08L5/08C08L33/02C08K3/22C07K1/14G01N21/64
Inventor 唐芳琼黄兴禄陈东
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products