Quantitative detection kit for helicobacter pylori nucleic acid

Abstract

The invention relates to a real-time fluorescent polymerase chain reaction detection kit for helicobacter pylori. The kit can quantitatively detect the helicobacter pylori in a gastric mucosa biopsy specimen. A detection method for the kit has the advantages of simple operation, short consumed time, high sensitivity and strong specificity, and can be widely used in various fields such as auxiliary diagnosis of helicobacter pylori infection, guide of clinical medication, epidemiology retrospective study, and the like.

Description

technical field [0001] The invention relates to a real-time fluorescent polymerase chain reaction detection kit for Helicobacter pylori. The kit uses real-time fluorescent PCR reaction to quantitatively detect Helicobacter pylori nucleic acid in gastric mucosal biopsy specimens, and can be used in many fields such as auxiliary diagnosis of Helicobacter pylori infection, guidance of clinical medication, and retrospective epidemiological research. Background technique [0002] Helicobacter pylori (Helicobacter pylori, Hp) is a kind of pathogenic bacterium of worldwide human infection, has a higher infection rate in the crowd, the infection rate in developed countries is about 50%, and the infection rate in developing countries can be as high as more than 80% (Jagadish C D and Nibedita Paul Epidemiolgy and Pathophysiology of Helicobacter pylori Infection in Children Indian Journal of Pediatrics, 2007, 74: 287-290.). The infection rate of Hp in the general population in my coun...

AI Technical Summary

Problems solved by technology

This method is mainly used for the study of Hp pathogenic mechanism and the identification of Hp cocciform bacteria, and generally cannot be used as a routine diagnostic method

(3) Bacterial culture, Hp microaerophilic bacteria, are sensitive to gas environment, temperature and culture medium type, etc., and the culture results are also affected by the above factors, so it is not easy to promote clinically

(4) 14C, 13C urea breath test, 15N-urine ammonia excretion test, rapid urease test may appear false positive

(5) Serum Hp antibody detection and fecal Hp antigen detection are currently commonly used clinical Hp antibody detection kits, which have the following defects: a. Due to the persistent systemic immune response, the detection process is not stable, and it is difficult to distinguish the infection stage; b. There are serum cross-reactions with Campylobacter jejuni and Campylobacter fetalis, and there are differences between different individual strains

Therefore, antigen preparation and strain selection all affect the detection results; c. Sensitivity and specificity are greatly affected by age. For patients over 30 years old, serological diagnosis is not recommended as a method for follow-up treatment results

Images