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Method for extracting paper mulberry alkaloid and application of paper mulberry alkaloid

An extraction method and alkaloid technology, applied in the field of phytochemistry, can solve problems such as discovery, and achieve the effect of simple extraction process, simple extraction and preparation process, and easy operation

Inactive Publication Date: 2010-01-13
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no reports have found such alkaloids from mulberry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 10kg of bark, crushed and boiled in water for 30 minutes to extract twice, 30L each time. The extract was left to cool to room temperature, and the supernatant was passed through a 732 cation exchange resin column (column volume 10L), washed with 10L of water, and then eluted with 30L of 1% ammonia / ethanol water (containing 70% ethanol) solution. The eluent was evaporated to dryness to obtain 50 g of total alkaloids, with a yield of 0.5%.

[0020] Get 3.5g of total bases, through silica gel column chromatography (chloroform-methanol gradient elution), C18 reverse phase column chromatography (water and methanol gradient elution) to obtain four compounds, by mass spectrum, carbon nuclear magnetic resonance spectrum and hydrogen spectrum identification These are broussonetine A and B and broussonetine A and B.

[0021] Get 50 g of the extract of the tree, add 3000 g of starch, 30 g of microcrystalline cellulose, granulate with 50% ethanol, and compress to obtain tablets, ...

Embodiment 2

[0023] 10kg of mulberry tree, crushed and boiled with 50% ethanol / water for 30 minutes to extract twice, 30L each time. After the extract was concentrated to recover ethanol, dilute hydrochloric acid was added to adjust the pH to 2, and then extracted with ethyl acetate. The aqueous phase was basified with ammonia to pH=10, then extracted with n-butanol. Recover n-butanol to obtain 80 g of off-white powder with a yield of 0.8%, which is the mulberry extract referred to in the present invention.

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Abstract

The invention belongs to the field of plant chemistry, in particular to a method for extracting active molecule alkaloid of paper mulberry and application of the alkaloid. The method comprises the following steps: taking roots or / and bark or / and leaves as raw materials, and extracting the raw materials with water or water-containing alcoholic solution at normal temperature or heating temperature; making the extracting solution pass strong acid type or weak acid type cation exchange resin, and adsorbing the active components of the extracting solution on the resin; washing a resin column with water, eluting the resin column with ammonia water / alcoholic solution, and concentrating and drying the eluent to obtain the total alkaloid of paper mulberry; and concentrating the extracting solution, extracting the concentration solution with ethyl acetate, alkalifying the water phase with alkali until the pH value is 12, extracting the water phase with n-butyl alcohol, and concentrating the extracting solution of n-butyl alcohol to obtain the total alkaloid of paper mulberry. The alkaloid of paper mulberry prepared by the method can be directly used or added with edible auxiliary materials to prepare a medicament or functional food capable of preventing diabetes and complications of the diabetes, or preventing obesity, or treating viral influenza. The method also has the advantages of simple process, low cost and easy industrialization.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and relates to a method for extracting alkaloids, an active component of mulberry tree, and its application in preventing and treating diabetes and the like. Background technique [0002] Diabetes mellitus is a progressive endocrine disease with glucose metabolism disorder as the main symptom group. The basic pathophysiology is the relative deficiency or absolute deficiency of insulin secretion function in the body or the abnormal function of insulin receptor, which causes metabolic disorder, including sugar, protein, Fat, water and electrolytes etc. The general manifestations are hyperglycemia, diabetes and hyperlipidemia. In severe cases, ketoacidemia may occur, and even circulatory attenuation and coma are life-threatening. Diabetes is divided into four categories, of which type II diabetes accounts for 90-95% of the total number of diabetes. [0003] Inactivity, obesity, and aging le...

Claims

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Application Information

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IPC IPC(8): A61K36/60A61P3/04A61P3/10A61P31/14A23L1/30A61K125/00A61K127/00A61K129/00A23L33/105
Inventor 王明奎鲍玲
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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