Kit test method of beta-lactam antibiotics residue in milk

Abstract

The invention discloses a kit test method of beta-lactam antibiotics residue in milk, comprising the following steps: performing once activation to bacillus stearothermophilus, then culturing the activated bacillus stearothermophilus in a production culture medium at 50-65 DEG C for 24-36h while stirring in a rotation speed of 100-150rpm, centrifugalizing to collect bacteria mire, adding agarose bromcresol purple solution, mixing evenly to ensure the number of spore in each kit reach 1.00*10<7>-2.50*10<8>cfu, subpackaging the spore in porous reaction vessels at 60-70 DEG C, covering aluminum-plastic film, placing upside down, sealing, placing the vessels in aluminum-plastic packages and storing in dark place while keeping from wet. The kit has the advantage of easy operation, high sensibility, good repeatability, low cost and high throughput, can be stored at 4-8 DEG C for 12-18 months and can be used to test beta-lactam antibiotics residue in milk.

Description

technical field [0001] The invention relates to the detection of antibiotic residues, in particular to a detection method of a kit for detecting β-lactam antibiotic residues in milk. Background technique [0002] In the dairy farming industry, β-lactam ring antibiotics are often used to treat mastitis in dairy cows, especially penicillin G is used in large quantities because of its broad spectrum and low cost, resulting in high concentrations of residues in milk. And penicillins can also cause very severe allergic reactions in some individuals. Long-term intake of milk containing such antibiotics will inhibit the normal flora in the human intestinal tract and cause people to develop drug resistance. In many countries, the management of β-lactam drugs in milk, especially penicillin G residues, is very strict. The limit of penicillin G in milk recommended by the World Health Organization has been increased from 0.05 μg / ml (50ppb) in 1976 to the current 0.004 μg / ml (4ppb), th...

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Problems solved by technology

Wang Zhiqiang et al. "Rapid Detection of Multiple Antibiotic Residues in Fresh Milk by Microbial Inhibition Method" (Chinese Journal of Food Hygiene, 2008, 20(2): 139-141) did not make a kit, the detection time was more than 24 hours, and the operation steps were complicated

[0004] Above-mentioned detection method all is to adopt microbiological detection method, generally exists due to the low (10 5 ~10 6 cfu / ml) and has poor thermal stability, low sensitivity, long detection time, and no kit is made, which makes the detection operation cumbersome and can not be standardized detection

However, enzyme-linked immunoassay and radioimmunoassay (also known as Charm II) require expensive testing instruments and are expensive. The testing cost of each sample is 100 to 300 yuan, which is not suitable for the low-cost testing needs of dairy companies.

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