Quantum dot-cyanine dye-folic acid biological probe and preparation method thereof
A technology of cyanine dyes and biological probes, which is applied in the field of quantum dot-cyanine dye biological probes and its preparation, can solve the problems of single fluorescent probes, and achieve the effects of simple preparation methods, high sensitivity, and less usage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0019] Quantum dot-cyanine dye biological probe preparation method of the present invention comprises the following steps:
[0020] 1) Preparation of quantum dots:
[0021] Synthesis of carboxyl or amino quantum dots: will contain Zn 2+ or CD 2+ The salt is dissolved in deionized water to form a solution to control the Zn 2+ or CD 2+ The concentration is 1.0~2.0×10 -2 mol / L and to Zn 2+ or CD 2+ Add an appropriate amount of thioglycolic acid or mercaptoethylamine dropwise into the solution and adjust the pH to 10-11 or 3-6 with NaOH or HCl solution, and add fresh transparent precursor dropwise under nitrogen protection conditions to make zinc selenide or cadmium selenide quantum dots , drop Na 2 SeSO 3 Or NaHSe solution, when making zinc telluride or cadmium telluride quantum dots, add Na 2 TeSO 3 Or NaHTe solution, when making zinc sulfide or cadmium sulfide quantum dots, add NaS solution dropwise, reflux reaction, and obtain a transparent quantum dot nucleus soluti...
Embodiment 1
[0032] Embodiment 1: Preparation of quantum dot-cyanine dye-folate bioprobe No. 1
[0033] 1) Preparation of quantum dots:
[0034] Synthesis of carboxyl quantum dots: 5.14gCdCl 2 2.5H 2 O was dissolved in 200mL of deionized water, and 2mL of thioglycolic acid was added dropwise to the solution, and the pH was adjusted to 10-11 with NaOH solution, and fresh transparent 0.00627molNa 2 SeSO 3 The precursor liquid, reflux reaction, obtains the transparent CdSe quantum dot core solution;
[0035] Preparation of quantum dot core-shell structure: 2.21g sodium sulfide and 0.24g CdCl 2 2.5H 2 The prepared O solution was added dropwise to the above CdSe quantum dot solution, and the ratio of the shell to the core was 3:1. Under nitrogen protection, control the temperature at 100°C for reflux reaction, cool down after the reaction is over, wash the aqueous solution of quantum dots with a shell-core structure in a beaker with acetone and anhydrous ether for 3 times, and centrifuge at...
Embodiment 2
[0045] Embodiment 2: Preparation of quantum dot-cyanine dye-folate bioprobe No. II
[0046] With the 0.00627mol Na of embodiment 1 step 1) 2 SeSO 3 The precursor solution was replaced with 0.00627molNa 2 TeSO 3 In 3), the above-mentioned cadmium sulfide-wrapped cadmium selenide is changed to cadmium sulfide-wrapped cadmium telluride, and other steps remain unchanged to obtain the quantum dot-cyanine dye-folate biological probe II;
[0047] With the 0.00627mol Na of embodiment 1 step 1) 2 SeSO 3 Change the precursor solution of 0.00627molNaHTe into the precursor solution of 0.00627molNaHTe, change the above-mentioned cadmium sulfide-wrapped cadmium selenide into cadmium sulfide-wrapped cadmium telluride in step 3), other steps can get quantum dot-cyanine dye-folic acid biodetection Needle No. II.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com