Quantum dot-cyanine dye-folic acid biological probe and preparation method thereof

A technology of cyanine dyes and biological probes, which is applied in the field of quantum dot-cyanine dye biological probes and its preparation, can solve the problems of single fluorescent probes, and achieve the effects of simple preparation methods, high sensitivity, and less usage

Active Publication Date: 2010-02-03
TIANJIN URBAN CONSTR COLLEGE
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  • Claims
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Problems solved by technology

[0008] In order to solve the problems existing in the above-mentioned technologies, the object of the present invention is to provide a kind of quantum dot-cyanine dye-folate biological probe and preparation method thereof, which is beneficial to solve the shortcoming of a single fluorescent probe, and fully embodies the organic dye and quantum dot It can accurately identify cancer cells, and has the characteristics of FRET, which can be used to study the spatial structure of folate receptor subtypes in cancer cells

Method used

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preparation example Construction

[0019] Quantum dot-cyanine dye biological probe preparation method of the present invention comprises the following steps:

[0020] 1) Preparation of quantum dots:

[0021] Synthesis of carboxyl or amino quantum dots: will contain Zn 2+ or CD 2+ The salt is dissolved in deionized water to form a solution to control the Zn 2+ or CD 2+ The concentration is 1.0~2.0×10 -2 mol / L and to Zn 2+ or CD 2+ Add an appropriate amount of thioglycolic acid or mercaptoethylamine dropwise into the solution and adjust the pH to 10-11 or 3-6 with NaOH or HCl solution, and add fresh transparent precursor dropwise under nitrogen protection conditions to make zinc selenide or cadmium selenide quantum dots , drop Na 2 SeSO 3 Or NaHSe solution, when making zinc telluride or cadmium telluride quantum dots, add Na 2 TeSO 3 Or NaHTe solution, when making zinc sulfide or cadmium sulfide quantum dots, add NaS solution dropwise, reflux reaction, and obtain a transparent quantum dot nucleus soluti...

Embodiment 1

[0032] Embodiment 1: Preparation of quantum dot-cyanine dye-folate bioprobe No. 1

[0033] 1) Preparation of quantum dots:

[0034] Synthesis of carboxyl quantum dots: 5.14gCdCl 2 2.5H 2 O was dissolved in 200mL of deionized water, and 2mL of thioglycolic acid was added dropwise to the solution, and the pH was adjusted to 10-11 with NaOH solution, and fresh transparent 0.00627molNa 2 SeSO 3 The precursor liquid, reflux reaction, obtains the transparent CdSe quantum dot core solution;

[0035] Preparation of quantum dot core-shell structure: 2.21g sodium sulfide and 0.24g CdCl 2 2.5H 2 The prepared O solution was added dropwise to the above CdSe quantum dot solution, and the ratio of the shell to the core was 3:1. Under nitrogen protection, control the temperature at 100°C for reflux reaction, cool down after the reaction is over, wash the aqueous solution of quantum dots with a shell-core structure in a beaker with acetone and anhydrous ether for 3 times, and centrifuge at...

Embodiment 2

[0045] Embodiment 2: Preparation of quantum dot-cyanine dye-folate bioprobe No. II

[0046] With the 0.00627mol Na of embodiment 1 step 1) 2 SeSO 3 The precursor solution was replaced with 0.00627molNa 2 TeSO 3 In 3), the above-mentioned cadmium sulfide-wrapped cadmium selenide is changed to cadmium sulfide-wrapped cadmium telluride, and other steps remain unchanged to obtain the quantum dot-cyanine dye-folate biological probe II;

[0047] With the 0.00627mol Na of embodiment 1 step 1) 2 SeSO 3 Change the precursor solution of 0.00627molNaHTe into the precursor solution of 0.00627molNaHTe, change the above-mentioned cadmium sulfide-wrapped cadmium selenide into cadmium sulfide-wrapped cadmium telluride in step 3), other steps can get quantum dot-cyanine dye-folic acid biodetection Needle No. II.

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Abstract

The invention provides a quantum dot-cyanine dye-folic acid biological probe. The probe structure comprises a probe which is formed by the quantum dot is bonded with the cyanine dye, and the cyanine dye is bonded with the folic acid. The invention further provides a preparation method of the quantum dot-cyanine dye-folic acid biological probe. The invention has the advantages of convenient preparation method and easy inducing quantum dot of the dye. The biological probe has the characteristics of targeted identifying tumor tissue and transferring fluorescence resonance energy, thereby not being capable of targeted identifying cancer cell and being capable of identifying the space location and the space structure of the cancer cell by the characteristic of transferring fluorescence resonance energy. The biological probe has the dual advantages of the quantum dot and the cyanine dye probe, and has simple preparation method, thereby being high-stability and high-efficiency targeted fluorescence mark. The probe has high sensitivity and less usage amount.

Description

technical field [0001] The invention relates to the interdisciplinary fields of chemistry, physics and biochemical technology, in particular to a quantum dot-cyanine dye biological probe and a preparation method thereof. Background technique [0002] Cancer is now the number one killer that threatens human health. Early diagnosis and effective identification of cancer can effectively improve the treatment of cancer. The shape and size of cells are different. Compared with normal cells, cancer cells are larger in volume, higher in nuclear-to-cytoplasmic ratio, vigorous in metabolism, higher in DNA and RNA polymerase activities, higher in DNA and RNA content, and significantly lower in nucleic acid decomposition process. normal cells. Folate receptors are highly expressed on the surface of most malignant tumor tissues, such as breast cancer, gastric cancer, ovarian cancer, endometrial cancer, colon cancer, nasopharyngeal cancer, laryngeal cancer, lung cancer, etc., while the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/56C09K11/06
Inventor 费学宁贾国治王军谷迎春
Owner TIANJIN URBAN CONSTR COLLEGE
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