Wound or tissue dressing comprising lactic acid bacteria

A technology of lactic acid bacteria and wounds, applied in bandages, absorbent pads, medical science, etc.

Inactive Publication Date: 2010-02-03
FERROSAN MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although they are waterproof, transparent film dressings are somewhat porous, allowing oxygen and moisture to pass through their barrier

Method used

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  • Wound or tissue dressing comprising lactic acid bacteria
  • Wound or tissue dressing comprising lactic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0205] Resuspend the lyophilized Lactobacillus GG in saline to a final concentration of 10 9 / ml. Knead the gelatin sponge (2×2cm) in 0.8ml Lactobacillus suspension or 0.8ml saline, and transfer it to Staphylococcus aureus, Psuedomonas aeruginosa or Coli Escherichia coli culture was plated on a 100 μl LB plate diluted by 1 / 10. Incubate the plate at 37°C. After 3 days, a growth inhibition (clear) zone was seen around the gelatin sponge kneaded with the Lactobacillus suspension, but not around the sponge kneaded with saline.

Embodiment 2

[0207] In a dual-compartment syringe, one compartment is filled with 10 ml of hydrogel, sealed and then autoclaved. After the sterilization process, fill the other compartment with appropriate low-humidity exhibitants (carboxymethyl cellulose and maltodextrin) and prebiotics (trehalose). 10 Lyophilized lactic acid bacteria. Grow a culture of Staphylococcus aureus, Pseudomonas aeruginosa or Escherichia coli to exponential growth and take 100ul of the culture to plate on the LB plate, at this time the culture reaches an OD of 0.5 600 . Holes are formed in the agar plate and the holes are filled with blank hydrogel or hydrogel containing lactic acid bacteria using a double-compartment syringe. Incubate the plate at 37°C. After 3 days, areas of growth inhibition (clarification) were seen around the pores of the Lactobacillus-containing hydrogel. No inhibition zone was seen around the pores containing smooth hydrogel.

Embodiment 3

[0209] Resuspend the lyophilized Lactobacillus GG in saline to a final concentration of 10 9 / ml, and fill 1ml of this suspension into a dialysis tube, where the molecular weight cut-off value is 300kDa, so that molecules up to 300kDa can pass through, but bacteria are retained in the tube. Add 1ml of saline to the other tube. Place these tubes on LB plates plated with 1 / 10 diluted 100ul on the culture of Staphylococcus aureus, Pseudomonas aeruginosa or Escherichia coli beforehand. Incubate the plate at 37°C. After 3 days, a growth inhibition (clear) zone was visible around the tube containing the Lactobacillus suspension, but not around the tube containing the saline.

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Abstract

The present invention is directed to a wound or tissue dressing comprising a bacteria having the property of producing lactic acid from sugars by fermentation of the sugars. The bacteria preferably belongs to the family of lactic acid bacteria. The family of lactic acid bacteria refers to any bacteria belonging to a genus selected from the group consisting of Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus and Weissella. There is also provided a wound or tissue dressing comprising an absorbent compound for absorbing wound exudate, wherein said wound or tissue dressing is attached to or comprises a lactic acid bacterium. The utility of the present invention is demonstrated by use of the wound or tissue dressings in methods for treating a wound or damaged tissue in an individual, said method comprising the steps of contacting said wound or damaged tissue with the wound or tissue dressing accordingto the invention, thereby treating the wound or damaged tissue. The treatment results in healing of the wound or in accelerated healing of the wound. There is also provided the use of a lactic acid bacteria in the manufacture of a wound or tissue dressing for treating or accelerating the healing of a wound in an individual.

Description

[0001] This patent application is a non-provisional patent application of US 60 / 870,625 filed on December 19, 2006 and its benefits are requested. This document is fully incorporated herein by reference. In addition, US 60 / 870,625 and all patent and non-patent references cited in this application are incorporated herein by reference. Invention field [0002] The present invention relates to a wound or tissue dressing containing lactic acid producing bacteria. The application of this type of wound or tissue dressing in healing wounds or accelerating wound healing is also provided. Background of the invention [0003] Wounds on animal skin and underlying tissues can be caused by, for example, friction, abrasion, tearing, compression, burns, or chemical irritation. Tissue damage can also be caused by metabolic or physical dysfunction in the body, including, but not limited to, bone protrudence, diabetes, circulatory insufficiency, or inflammatory processes. [0004] Wounds can be cla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/36
Inventor J·E·翰森L·K·杰斯皮尔森
Owner FERROSAN MEDICAL DEVICES
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