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Tissue-culture rapid-propagation method of double-happiness cuckoo variety and special culture medium thereof

A technique for the tissue culture of Rhododendron xilinmen, which is applied in the field of plant tissue culture and propagation, can solve the problems that the characteristics of the Rhododendron xilinmen species cannot be reproduced, there are no reports, and there is a lack of earing materials, so as to help the branches to be strong and reduce production. Low cost and good light transmittance

Active Publication Date: 2010-02-17
YUNNAN GREEN LAND BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because its provenance comes from budding branches, there is a lack of earing materials for cuttings, and cutting propagation is affected by seasons and rooting survival rates, and the propagation speed is slow, making it difficult to carry out large-scale production and meet the needs of large-scale application; relying on seeds The breeding offspring are seriously separated, and the variety characteristics of "Xilinmen" Rhododendron cannot be reproduced. Therefore, it is of great significance to develop a special medium for tissue culture and a method for rapid propagation of tissue culture for this fine variety.
[0005] At present, there is no report on the tissue culture research on the variety "Rhododendron xilinmen"

Method used

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  • Tissue-culture rapid-propagation method of double-happiness cuckoo variety and special culture medium thereof

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Embodiment 1

[0036] Embodiment 1, the tissue culture rapid propagation method of Rhododendron xilinmen variety and its special medium test

[0037] The test materials were obtained from the selected individual Rhododendron "Xilinmen" in the greenhouse of Yunnan Lvdadi Biotechnology Co., Ltd. The reagents used: MS medium was prepared by our company; ZT (ie zeatin) and NAA (naphthalene acetic acid) were obtained from Beijing Kangbei Tech Ltd purchased.

[0038] The special medium for Rhododendron xilinmen consists of induction medium for adventitious buds, medium for proliferation of adventitious buds, medium for subculture and strong seedlings of clustered seedlings, and medium for rooting:

[0039] Wherein, the formula of the induction medium of adventitious buds is: 1 / 4MS, ZT3mg / L, NAA0.5mg / L, sugar 30g / L and agar 5g / L, pH is 5.4-5.6;

[0040] The formula of the proliferation medium of described adventitious buds is: 1 / 4MS, ZT1mg / L, NAA 0.5mg / L, sugar 30g / L and agar 5g / L, pH is 5.4-5.6;

...

Embodiment 2

[0051] The experiment of embodiment 2 explant sterilization method

[0052] Because of the biological characteristics of the variety "Azalea Xilinmen", it is difficult to establish an aseptic system in bottles, so this study conducted an experiment on the sterilization method of the explants of the variety "Azalea Xilinmen".

[0053] In this experiment, two kinds of disinfectant liquids and different sterilization times were used to disinfect the stem tips and stem segments of the bagged sprouting branches of the "Xilinmen Rhododendron" variety, and to find the best disinfection treatment method; using bagged and unbagged The newly germinated young shoots were compared with the treatment time of the two disinfectants. Seen from Table 1:

[0054] (1) The explant material was treated with 75% alcohol for 10-30min, and then treated with 0.1% HgCl 2 Soak the tips for 5 minutes and soak the stems for 8-10 minutes, and the effective seeding rate will reach 75%-90%;

[0055] (2) T...

Embodiment 3

[0059] Example 3 Screening experiment of differentiation induction medium

[0060] In this experiment, through different base media and hormone ratios of different concentrations, the media formulations to improve the explant survival rate, differentiation rate, induction rate of clustered seedlings and strong seedlings of "Rhododendron xilinmen" were searched; NAA and IBA were selected as two auxin , mixed and used alone to screen the best rooting medium formula.

[0061] In this experiment, the Read medium with simple basic elements and relatively low concentration was selected as the control, and the concentration of inorganic elements in MS medium was reduced to 1 / 4, as the basic medium for screening the tissue culture of the "Xilinmen Rhododendron" variety. Using the combination of ZT 3mg / l and NAA 0.5mg / l and concentration limitation, a good effect of 100% induction rate of tissue-cultured buds of Rhododendron "Xilinmen" was obtained, which is 50-80% higher than the effe...

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Abstract

The invention discloses a tissue-culture rapid-propagation method of a double-happiness cuckoo variety and a special culture medium thereof, belonging to the technical field of the tissue culture andthe propagation of plants. The special culture medium comprises an adventitious bud inducement culture medium, an inducement propagation culture medium, a clustered seedling successive and sound-seedling culture medium and a rooting culture medium, wherein the adventitious bud inducement culture medium comprises 1 / 4MS, 3mg / L ZT, 0.5mg / L NAA, 30g / L sugar and 5g / L agaragar; the inducement propagation culture medium comprises 1 / 4MS, 1mg / L ZT, 0.5mg / L NAA, 30g / L sugar and 5g / L agaragar; the clustered seedling successive and sound-seedling culture medium comprises 1 / 4MS, 0.5 mg / L ZT, 0.5mg / L NAA, 30g / L sugar and 5g / L agaragar; the rooting culture medium comprises 1 / 4MS, 2mg / L IBA, 30g / L sugar and 5g / L agaragar; and pH values of all culture media are 5.4-5.6. The tissue-culture rapid-propagationmethod has a seedling transplantation survival rate more than 90 percent and can achieve a reproductive capacity higher than 0.1 million plants / year.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and propagation, in particular to a method for tissue culture and rapid propagation of the "Xilinmen" rhododendron variety. Background technique [0002] "Xilinmen Rhododendron" is the variety name of this Rhododendron species. "Xilinmen Rhododendron" belongs to Rhododendronaceae, a small evergreen shrub of the genus Rhododendron, which is cultivated from the bud mutation of R.pulchrum sweet. This variety obtained the Chinese plant variety right on September 7, 2007. The variety right number: 20070023, the variety name: Rhododendron xilinmen, and the variety right owner: Yunnan Lvdadi Biotechnology Co., Ltd., which is also the applicant for this patent application people. [0003] The "Xilinmen Rhododendron" variety has biological characteristics such as strigillose explants, which makes it difficult to establish an aseptic system in bottles during tissue culture. After the aseptic ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何学葵陈德生朱光洁胡磊童亚丽付慧慧宣艳辉
Owner YUNNAN GREEN LAND BIOLOGICAL TECH
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