The invention provides a primer and a method for rapidly detecting the sex of
juvenile grass carp, and relates to the technical field of biological detection. The method for rapidly detecting the sex of the
juvenile grass carps specifically comprises the following steps: step 1, sample acquisition: acquiring a plurality of grass carps, determining male and female individuals in a
gonad form, respectively extracting tissue samples from the male and female individuals, and preserving the tissue samples at low temperature; step 2, extracting the total
DNA of the sample to be detected: extracting muscular tissue
genome DNA of the three female fishes and the three male fishes by using a TIANAmp Marine Animal
DNA kit, measuring the concentration of the DNA and the ratio of OD260 / OD280 by using a NanoDrop2000 ultramicro spectrophotometer, and detecting the integrity of the DNA by using 1.0%
agarose electrophoresis; and step 3, primer detection. The primer and the detection method provided by the invention can avoid the problems that the difficulty of taking the
gonad in conventional fish killing is relatively large, the taken
gonad also needs to be subjected to
paraffin section to identify the sex, and the process is tedious, and realize that the sex of the
grass carp fries can be identified only through one-time PCR amplification and
agarose gel electrophoresis detection.