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Immobilized cultivation method for 'microbiological straw additive'

A technology of biological additives and immobilized culture, which is applied in the direction of fixing on/in the organic carrier, can solve the problems of large manpower, material resources and poor production stability, affecting the process of industrialization, etc.

Inactive Publication Date: 2010-03-03
天津市农业科学院
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Problems solved by technology

However, in the batch production of the bacterial agent, there are disadvantages such as a large amount of manpower, material resources for seed production, poor production stability, and high production costs, which affect the industrialization process of this patent, and the widely used immobilization technology just makes up for it. these deficiencies

Method used

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Embodiment Construction

[0012] This item provides an immobilized culture method for "micro-storage biological additives of straw" which is suitable for the fermentation of "micro-storage biological additives of straw".

[0013] The process is: add the activated "straw micro-storage biological additive" bacterial agent into the proliferation medium, (the formula per liter of the proliferation medium is: 7.5g of yeast extract, 10g of glucose, 7.5g of peptone, 2g of potassium dihydrogen phosphate, tomato Juice 100ml, pH 6.5-7.0)

[0014] Boil 8% polyvinyl alcohol and 2% sodium alginate of the immobilized embedding carrier in hot water, mix them in a volume ratio of 0.6:1.4, and use Ca(OH) 2 The pH value of the solution was adjusted to 7.2, cooled and multiplied after sterilization for 18 hours (the concentration of the bacterial solution was 10 9 cfu / ml) of the mixed fermentation bacteria agent is mixed according to the volume ratio of 1:1, and the embedding agent mixed with the bacteria is dripped int...

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Abstract

The invention discloses an immobilized cultivation method for 'microbiological straw additive', which comprises the following steps: mixing an activated 'microbiological straw additive' bacterial agent with an immobilized embedded carrier (mixture of 8 percent polyvinyl alcohol and 2 percent sodium alginate in a volume ratio of 0.6 to 1.4) in a volume ratio of 1 to 1, then dropping an embedding agent mixed with bacteria into the sterilized 0.2M CaCl2 solution in a super clean bench by using a medical injector of 10 milliliters and a matched needle, controlling the dropping speed, forming granules to embed the mixed bacterial agent therein, soaking the granules in the CaCl2 solution, flushing the granules by sterile water, and adding a proliferation culture medium into the solution to carryout timing cultivation. The method can fully exert the activity of strains, improve the tolerance of the strains, improve the operation stability of the bacterial agent production, simplify the operation process, simplify the reclamation problem of microbe at the later stage of the reaction, reduce the production cost and labor intensity, fully exert the advantages of microbe reaction, realize on-line regeneration of the microbe, and ensure the feasibility of the process.

Description

technical field [0001] The invention belongs to a microbial fermentation method in bioengineering, in particular to a method for immobilized cultivation of "stalk micro-storage biological additive". Background technique [0002] This patent is an improved research on production technology based on the patent "Straw Fermentation Biological Additive and Its Preparation Method" (Patent No. ZL97119176) (hereinafter referred to as: Straw Micro-storage Biological Additive). "Straw micro-storage biological additive" can turn half-yellow or dry-yellow crop straw into roughage for ruminant livestock with sour smell, moist softness and good palatability within 7-10 days. The patent has good economic and social benefits. However, in the mass production of the bacterial agent, there are disadvantages such as a large amount of manpower, material resources for seed production, poor production stability, and high production costs, which affect the industrialization process of this patent,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04
Inventor 范寰
Owner 天津市农业科学院
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