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Application of usnic acid in anti-influenza virus medicaments

An anti-influenza virus and influenza virus technology, applied in the field of medicine, can solve problems such as the absence of usnic acid, and achieve the effects of alleviating treatment pain, improving treatment quality, medication compliance, and improving tolerance

Inactive Publication Date: 2010-04-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no application of usnic acid (UA) in the anti-influenza virus in the current literature reports.

Method used

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  • Application of usnic acid in anti-influenza virus medicaments
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  • Application of usnic acid in anti-influenza virus medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Evaluation of usnic acid anti-influenza virus activity

[0043] I. Test material

[0044] 1. Cells, viruses and plasmids:

[0045] MDCK cells were provided by Shandong Institute of Medical Microbiology, Chinese Academy of Sciences; A549 cells, HeLa cells, virus H5N1 or H3N2 were purchased from China Collection Center for Typical Microorganisms; pM-scFv, pVP-16-NP, pM-PB1, pM-PB2, pVP- The 16-NP and pG5Luc plasmids were completed under the guidance of Professor Wu Jianguo from the Medical Virology Research Group of the State Key Laboratory of Virology, Wuhan University. For detailed information, please refer to the published papers of this laboratory—Mukhtar, M.M., Li, S., Li, W., Wan, T., Mu, Y., Wei, W., Kang, L., Rasool, S.T., Xiao, Y., Zhu, Y., and Wu, J. (2009). Single-chain intracellular Antibodies inhibit influenza virus replication by disrupting interaction of proteins involved in viral replication and transcription. Int J Biochem Cell Biol 41(3),...

Embodiment 2

[0147] Example 2: Usnic acid prevents viral infection, and influences on influenza virus adsorption invasion and replication

[0148] The cells paved into a single layer were treated in different ways: the drug was added to the cells in advance and incubated for 1 hour, the medium was changed, and the corresponding influenza virus was added; the drug was added to the virus for adsorption and invasion of the influenza virus, and the drug was added to the virus and incubated for 1 hour, and then Then add it to the cells; for the effect of the drug on the replication of the influenza virus, the virus is first used to infect the cells for 1 hour, and then the drug is added. The drug concentration used in all experiments was 100 μg / ml, and the virus infection amount was 1M.O.I virus liquid. Part of the supernatant was collected at 24h, 36h, and 48h respectively, and the antiviral effect of the drug was determined by HA.

[0149] see results Figure 9-1 , 9-2 and 9-3, indicating ...

Embodiment 3

[0150] Embodiment 3: The influence of usnic acid on some enzymes in the replication process is explored by two-hybrid experiments in mammals

[0151] (1) Cell transfection

[0152] The plasmids are pM-PB1, pM-PB2, pVP-16-NP, pG5Luc, respectively. In this test group, no plasmids were added to the negative control, pM-PB1 (or pM-PB2), pVP-16-NP, and pG5Luc were added to the positive control, and pM-PB1 (or pM-PB2), pVP- 16-NP, pG5Luc three plasmids and various drugs.

[0153] Sofast TM lipofection

[0154] The following transfection operation uses a 24-well plate as an example. The whole process of transfection must be strictly aseptic.

[0155] 1. Seed the cells to be transfected in a 24-well plate one day before transfection and culture at 37°C. The cell density before transfection was 40-60%.

[0156] 2. Prepare the following solutions

[0157] ① Dilute 1.5μl Sofast TM Reagents in 30 μl serum-free, antibiotic-free DMEM medium. After mixing, let stand at room tempera...

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Abstract

The invention discloses application of usnic acid (UA) in anti-influenza virus medicaments. The invention shows that the usnic acid has a certain anti-influenza virus function in vitro on MDCK cells and A549 cells infected by influenza A viruses, such as H5N1, H3N2 and the like. The impact effect of the medicaments applying the usnic acid relatively approaches that of a positive control medicament, namely amantadine; in addition, the usnic acid has better inhibiting effect on inflammatory reaction induced by the influenza viruses, which exposes that the medicaments applying the usnic acid have the prospect of being developed into anti-influenza virus medicaments.

Description

technical field [0001] The invention relates to the application of usnic acid in anti-influenza virus drugs, and belongs to the technical field of medicine. Background technique [0002] Influenza virus is the pathogen that causes influenza, which is divided into three types: A virus, influenza B virus and influenza C virus. Among them, influenza A is the most threatening to humans. Human influenza viruses are mainly H1, H2 and H3 subtypes, while the highly pathogenic avian influenza viruses currently causing serious harm are mostly H5, H7 and H9 subtypes, namely H5N1, H7N7, H9N2, etc., among which the case fatality rate of H5N1 subtype high. [0003] Influenza virus belongs to the Orthomyxoviridae family (Orthomyxoviridae), has 8 fragments of the envelope, and is a single-stranded negative RNA virus encoding 11 proteins. Unlike other RNA viruses, the synthesis of all RNA (mRNA, cRNA, vRNA) of influenza virus is completed in the nucleus of infected cells. After the mRNA i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P31/16
Inventor 吴建国陶君彦朱应李崇明黄志军袁婷婷
Owner WUHAN UNIV
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