The invention provides a high-yield
lichen acid
engineering bacterium YAFWY001 and a culture method thereof, and relates to the technical field of microbial
biosynthesis. The invention provides an
engineering bacterium capable of producing serotonic acid, the
engineering bacterium is prepared by transferring a yARGAorsA expression element into a pyrG
gene-deleted
aspergillus nidulans strain, the yARGAorsA expression element is formed by linking genes Ya5, pyrG, Rib, gbdA (p), OrsA and Ya3 in sequence from the 5end, and the engineering bacterium capable of producing serotonic acid provided by the invention is characterized in that
magnesium acetate (YGM) is added into a YG
liquid culture medium, so that the engineering bacterium capable of producing serotonic acid is obtained. The content of the
lichen acid in the
fermentation liquor of the
lichen acid producing engineering strain can be obviously increased, and the method is a new way for obtaining the
lichen acid at low cost.