Method for extracting ellagic acid in pomegranate peel residue by using biological enzyme

A technology of pomegranate peel and ellagic acid, which is applied in the fields of medicine, food, and chemical industry, and can solve problems such as adverse environmental effects, and achieve the effects of shortening production time, achieving environmental friendliness, and strong selectivity

Inactive Publication Date: 2010-05-05
CHINA AGRI UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of organic reagents, it will cause adverse effects on the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Extraction of ellagitannins

[0031] After pulverizing the dried pomegranate peel, add it to water, wherein the mass ratio of pomegranate peel residue to water is 1:15, put it under the action of ultrasonic waves with a power of 300W, and perform ultrasonic treatment for 3 times, each time for 45 minutes, to obtain ellagitanthin An aqueous solution of Ning. The content of ellagitannin in the above aqueous solution of ellagitannin was determined by Foline-Phenol colorimetry. The content of ellagitannin in the obtained ellagitannin aqueous solution was 10.19 g / L.

[0032] (2) Enzymolysis reaction

[0033] The aqueous solution of ellagitannin obtained in step (1) was adjusted to pH 5.0 with NaOH aqueous solution, and then 158.96 U of xylanase (that is, the enzyme dosage per gram of ellagitannin was degraded was 15.6 U), Penta Glycanase 5.1U (that is, the amount of enzyme that degrades ellagitannin per gram is 0.5U) and tannase 20.4U (that is, the amount of enzyme th...

Embodiment 2

[0037] (1) Extraction of ellagitannins

[0038] After pulverizing the dried pomegranate peel, add it to water, wherein the volume ratio of the mass of pomegranate peel residue to water is 1:20, place it under the action of ultrasonic waves with a power of 330W, and perform ultrasonic treatment for 3 times, each time for 35 minutes, to obtain tanned An aqueous solution of flower tannins. The content of ellagitannin in the above aqueous solution of ellagitannin was determined by Foline-Phenol colorimetry. The content of ellagitannin in the obtained ellagitannin aqueous solution was 8.90 g / L.

[0039] (2) Enzymolysis reaction

[0040] Adjust the pH of the aqueous solution of ellagitannin obtained in step (1) to 5.3 with NaOH aqueous solution, and then add 92.56 U of xylanase (that is, the enzyme dosage for degrading each gram of ellagitannin is 10.4 U), SPE -007 0.89U (that is, the amount of enzyme that degrades ellagitannin per gram is 0.1U) and compound plant hydrolase 2.848...

Embodiment 3

[0044] (1) Extraction of ellagitannins

[0045] After pulverizing the dried pomegranate peel, add it to water, wherein the volume ratio of the mass of pomegranate peel residue to water is 1:30, put it under the action of ultrasonic waves with a power of 350W, and perform ultrasonic treatment twice, each time for 30 minutes, to obtain tanned An aqueous solution of flower tannins. The content of ellagitannin in the above aqueous solution of ellagitannin was determined by Foline-Phenol colorimetry. The content of ellagitannin in the obtained ellagitannin aqueous solution was 5.62 g / L.

[0046] (2) Enzymolysis reaction

[0047]Adjust the pH of the aqueous solution of ellagitannin obtained in step (1) to 5.5 with NaOH aqueous solution, and then add 8.767U of xylanase (that is, the enzyme dosage per gram of ellagitannin is 1.56U), SPE -0070.281U (that is, the amount of enzyme that degrades ellagitannin per gram is 0.05U) and tannase 1.124U (that is, the amount of enzyme that degr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for extracting ellagic acid in pomegranate peel residue by using biological enzyme, belonging to the fields of food, medicine and chemical engineering. The method takes the pomegranate peel residue as raw material and water as solvent, and can be used for preparing the ellagic acid by using the biological enzyme. Biological compound enzyme of degradable ellagitannin can be used for carrying out enzymolysis reaction on the ellagitannin, and alkaline liquor is used for stopping the enzymolysis reaction; simultaneously, an alkaline water system can be formed for extracting the ellagic acid, and crude product of the ellagic acid can be obtained; and the extraction rate of the ellagic acid reaches 12.69-24% based on the content of the ellagitannin obtained in the step (1). The method has simple and continuous technique, environment-friendly manufacturing process, environmental protection and less discharge of the three wastes, and is suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the fields of food, medicine and chemical industry, and in particular relates to a method for extracting ellagic acid in pomegranate peel dregs with biological enzymes. Background technique [0002] Ellagic acid (2,3,7,8-tet rahydroxy benzopyrano[5,4,3cde]benzopyran-5,10-dione) is a dimeric derivative of gallic acid, which is widely present in various soft fruits, Natural polyphenolic substances in plant tissues such as nuts. In nature, it can not only exist in free form, but more in the form of easily hydrolyzed ellagitannins. The molecular formula for ellagic acid is C 14 h 6 o 8 , Slightly soluble in water and alcohol, soluble in alkali and pyridine, insoluble in ether. Pure ellagic acid is a yellow needle-like crystal with a melting point (pyridine) greater than 360°C. [0003] The polyhydroxy structure of ellagic acid determines that it has various physiological activities. Studies have shown that ellagic acid has g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18
Inventor 张燕姜斌胡小松廖小军
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap