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Method for industrially preparing sodium alginate micro-capsule loading high-density cells

A sodium alginate, high-density technology, applied in microcapsules, capsule delivery, pharmaceutical formulations, etc., can solve the problem of not being able to meet the needs of industrial production, wide particle size distribution of sodium alginate microcapsules, and failure to achieve high-density microencapsulation Cells and other problems, to achieve the effect of narrow particle size distribution range, uniform particle size distribution of microcapsules, and concentrated particle size distribution of microcapsules

Inactive Publication Date: 2011-07-06
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The high-voltage electrostatic droplet method has the advantages of being simple and easy, and requires less sample, but the main problems are: (1) the production efficiency of microcapsules is low, which is far from meeting the needs of industrial production; (2) the prepared sodium alginate microcapsules Capsule particle size distribution is wide, generally 100-600 μm
[0009] However, in the process of experiment and production, we found that the preparation of sodium alginate microcapsules by the vibrating nozzle method has the disadvantage of low density of encapsulated cells, which cannot meet the requirements of high-density microencapsulated cells, which hinders the method of microencapsulated cells Further application in engineering pilot test and industrial production
This shortcoming is mainly manifested as follows: when the cell density is low, the obtained microcapsules have uniform size and good capsule shape; when the cell density is high, the obtained microcapsules have poor capsule shape, large difference in particle size, and wide particle size distribution

Method used

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  • Method for industrially preparing sodium alginate micro-capsule loading high-density cells
  • Method for industrially preparing sodium alginate micro-capsule loading high-density cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of sodium alginate solution

[0024] Weigh 20 g of sodium alginate, dissolve it in 100 ml of sterilized physiological saline, and filter aseptically through a 0.22 μm microporous membrane to obtain a 2% sodium alginate solution.

[0025] (2) Cells are mixed with sodium alginate solution

[0026]Divide the total number of cells to 5 x 10 7 One bovine adrenal medulla chromaffin cells (BCC) and 10ml of 2% sodium alginate solution were prepared into a suspension by stirring.

[0027] (3) Preparation of calcium alginate microspheres

[0028] Using the Inotech IE-50R Encapsulator microcapsule instrument, using the vibrating nozzle method (Vibrating jetbreakup), by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, spray the suspension to 300ml with a concentration of 100mmol / L containing 0.5% polo Sham(F68)CaCl 2 In the solution, calcium alginate microspheres with a diameter in the range of 200-40...

Embodiment 2

[0037] (1) Preparation of sodium alginate solution

[0038] Weigh 22 g of sodium alginate, dissolve it in 100 ml of sterilized physiological saline, and filter aseptically through a 0.22 μm microporous membrane to obtain a 2.2% sodium alginate solution.

[0039] (2) Cells are mixed with sodium alginate solution

[0040] Divide the total number of cells to 10 x 10 7 One bovine adrenal medulla chromaffin cells (BCC) and 10 ml of 2.2% sodium alginate solution were prepared into a suspension by stirring.

[0041] (3) Preparation of calcium alginate microspheres

[0042] Inotech IE-50R Encapsulator microcapsule instrument, using the vibrating jet breakup method (Vibrating jet breakup), by adjusting the size of the nozzle, controlling the flow rate, vibration frequency and other process parameters, spray the suspension to 300ml with a concentration of 100mmol / L containing 0.2% HSA (for injection) CaCl 2 In the solution, calcium alginate microspheres with a diameter in the range ...

Embodiment 3

[0050] Embodiment 3: each step is equal to embodiment 1 or 2, but different therefrom, the concentration of the sodium alginate solution obtained in the preparation of step (1) sodium alginate solution is 3%.

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Abstract

The present invention discloses a method for industrially preparing sodium alginate micro-capsule loading high-density cells, which is a method for preparing the sodium alginate micro-capsule and packing cells by using sodium alginate-polylysine or chitosan-sodium alginate as a composite packing material through a vibrating nozzle method. The method increases the concentration of the sodium alginate to 1.5-5% in the preparation process of the sodium alginate micro-capsule and adds a surfactant into the curing solution of the micro-capsule so as to enhance the cell packing density in the sodium alginate micro-capsule and improve the shape of the micro-capsule and the particle size distribution. The method is capable of obviously enhancing the density of the cells in the sodium alginate micro-capsule and improving the shape of the micro-capsule, has uniform particle size distribution of the micro-capsule and improves production efficiency so as to meet the requirements of the micro-capsule on industrial production.

Description

technical field [0001] The invention relates to the field of biomedicine of microencapsulated cell engineering, more specifically, relates to a new method for industrially preparing large quantities of sodium alginate microcapsules loaded with high-density cells and improving the shape of the capsules. Background technique [0002] Microcapsules are used to encapsulate tissue cells with specific functions to form immune-isolated artificial cells, which can be implanted into diseased animals or patients to supplement the lack of physiologically active substances in the diseased body, so as to achieve the purpose of treating diseases. The three-layer structure membrane microcapsule made of sodium alginate-polylysine-sodium alginate (APA) or sodium alginate-chitosan-sodium alginate (ACA) has a small volume (particle size range: 200~ 1000μm), good biocompatibility, large surface area, which is conducive to the long-term survival of microencapsulated cells in animals. A large nu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/36A61K47/42A61K35/12A61K35/15
Inventor 刘善奎景颖王海龙张爱兵龚非
Owner UNIV OF JINAN
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