Bioluminescent nanoparticle, temperature sensing film and preparation method thereof
A fluorescent nanoparticle, sensing thin film technology, applied in chemical instruments and methods, thermometers with physical/chemical changes, thermometers, etc., can solve the problems of lack of biocompatibility, large sensor volume, and insufficient temperature resolution.
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[0047] Materials used: ethyl 2-naphthoate, 2′-naphthylethanone, sodium hydride, trioctylphosphine oxide, europium trichloride, polymethyl methacrylate, polyvinyl alcohol and filter membrane were purchased from Sigma-Aldrich , 2-bis(trimethoxysilyl)decane was purchased from Gelest Company, all of which were of analytical grade. Ethanol, sodium hydroxide and hydrochloric acid are analytically pure, and the water used is double distilled water.
[0048] Step 1: Synthesize DNM
[0049] Dissolve 3.40g (20mmol) of ethyl 2-naphthoate and 3.72g (20mmol) of 2'-naphthyl ethyl ketone into 100ml of tetrahydrofuran, and then add 1.06g (44mmol, 10% excess) of sodium hydride gradually while stirring. The resulting mixture was heated at reflux at 67°C for 3 hours, then cooled at room temperature for 24 hours. An appropriate amount of water (1-2 ml) was added to the solution, followed by acidification with 0.1 mol HCl. The obtained crude product was extracted with diethyl ether, and purifie...
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