Bioluminescent nanoparticle, temperature sensing film and preparation method thereof

A fluorescent nanoparticle, sensing thin film technology, applied in chemical instruments and methods, thermometers with physical/chemical changes, thermometers, etc., can solve the problems of lack of biocompatibility, large sensor volume, and insufficient temperature resolution.

Inactive Publication Date: 2010-06-09
BEIJING JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the physiological range (25-45 °C) and in vivo detection, the current temperature sensor is limited by the following factors: (1) the temperature resolution is not high enough, (2) lack of biocompatibility, (3) the volume of the sensor is too big
Therefore, in vivo temperature measurement methods and materials are rarely reported.

Method used

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  • Bioluminescent nanoparticle, temperature sensing film and preparation method thereof
  • Bioluminescent nanoparticle, temperature sensing film and preparation method thereof
  • Bioluminescent nanoparticle, temperature sensing film and preparation method thereof

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Embodiment Construction

[0047] Materials used: ethyl 2-naphthoate, 2′-naphthylethanone, sodium hydride, trioctylphosphine oxide, europium trichloride, polymethyl methacrylate, polyvinyl alcohol and filter membrane were purchased from Sigma-Aldrich , 2-bis(trimethoxysilyl)decane was purchased from Gelest Company, all of which were of analytical grade. Ethanol, sodium hydroxide and hydrochloric acid are analytically pure, and the water used is double distilled water.

[0048] Step 1: Synthesize DNM

[0049] Dissolve 3.40g (20mmol) of ethyl 2-naphthoate and 3.72g (20mmol) of 2'-naphthyl ethyl ketone into 100ml of tetrahydrofuran, and then add 1.06g (44mmol, 10% excess) of sodium hydride gradually while stirring. The resulting mixture was heated at reflux at 67°C for 3 hours, then cooled at room temperature for 24 hours. An appropriate amount of water (1-2 ml) was added to the solution, followed by acidification with 0.1 mol HCl. The obtained crude product was extracted with diethyl ether, and purifie...

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Abstract

The invention relates to a bioluminescent nanoparticle, a temperature sensing film and a preparation method thereof, and belongs to the technical field of biological temperature sensors. The bioluminescent nanoparticle comprises a silicon dioxide shell and a hybrid core, wherein the hybrid core is formed by randomly dispersing Eu-DT in 2-bis(trimethoxysilyl)decane and polymethyl methacrylate; and the silicon dioxide shell is formed by performing hydrolytie polycondensation on the 2-bis(trimethoxysilyl)decane. The bioluminescent temperature sensing film comprises the bioluminescent nanoparticles and PVA film vectors in a weight ratio of 0.01 to 0.03 percent. The sensing film has tiny particle volume and excellent temperature resolution ratio; and the Eu-DT probe is encapsulated in a nanometer protective layer which cannot cause organism repulsion, so that the material has good organism compatibility, and the encapsulation ensures that the probe has resistance to external interference.

Description

technical field [0001] The invention relates to a bioluminescent nanoparticle and a temperature sensing film and a preparation method thereof. The bioluminescent nanoparticle and the temperature sensing film with a core-shell structure have temperature sensing characteristics and belong to the technical field of biological temperature sensors. Background technique [0002] Temperature detection plays a very important role in scientific research, industry, medicine and other fields. Among the various methods of detecting temperature, the temperature detection method based on the principle of luminescence has attracted much attention, because this method can be non-invasive, accurate, and will not be disturbed in a strong electromagnetic field. There is a certain market demand for biological temperature sensors based on the principle of luminescence. For example, in local hyperthermia and photodynamic therapy of tumors, obtaining temperature information of target cells and tis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01K11/32
Inventor 彭洪尚谢蒂旎黄世华
Owner BEIJING JIAOTONG UNIV
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