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Preparation method of T4 bacteriophage supported precious metal nano-particle catalyst

A nanoparticle and bacteriophage technology, applied in the field of catalysis, can solve the problems of reducing catalytic activity and increasing production cost, and achieve the effects of high electrocatalytic activity, reduced economic cost and small size

Inactive Publication Date: 2011-11-16
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the steric hindrance effect of the pores, the noble metal nanoparticles cannot fully contact the catalyzed system, thereby reducing the catalytic activity.
Moreover, the preparation of the porous oxide carrier itself also requires a series of finely controlled processes, which increases the production cost.

Method used

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  • Preparation method of T4 bacteriophage supported precious metal nano-particle catalyst
  • Preparation method of T4 bacteriophage supported precious metal nano-particle catalyst
  • Preparation method of T4 bacteriophage supported precious metal nano-particle catalyst

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Proliferation and enrichment of T4 phage

[0028] The T4 phage was inserted into 50 mL of pre-incubated E. coli suspension with a concentration of 10 mg / mL, and cultured on a shaking table at 37° C. at 120 r / min for 12 h. The obtained suspension containing T4 phage and Escherichia coli fragments was centrifuged at 4°C for 15 min at 4000, 5000, and 6000 r / min in sequence, and the precipitate was discarded each time, and the supernatant was taken. Then the supernatant containing pure T4 phage was ultracentrifuged at 4°C and 45000r / min for 3h. The obtained T4 phage precipitate was collected and dispersed in 2.5 mL deionized water. Its morphology was observed under a transmission electron microscope. figure 2 .

[0029] 2. Preparation of T4 phage-loaded Pt nanoparticles catalyst

[0030] Add 200 μL of the enriched T4 phage suspension to 200 μL of 5 mM PtCl 4 Mix well in the solution, and incubate at 4°C for 10 hours on a shaker at 60 r / min. Afterwards, ultracentri...

Embodiment 2

[0032] 1. Proliferation and enrichment of T4 phage

[0033] See step 1 of Embodiment 1, the process is exactly the same.

[0034]2. Preparation of T4 phage-loaded Rh nanoparticles catalyst

[0035] Add 200 μL of the enriched T4 phage suspension to 200 μL of 5 mM RhCl 3 Mix well in the solution, and incubate at 4°C for 10 hours on a shaker at 60 r / min. Afterwards, ultracentrifuge at 4°C, 45000r / min for 3h, collect the obtained precipitate, and disperse in 200μL deionized water. Then add 75 μL freshly prepared 5 mM NaBH dropwise 4 solution, that is, the T4 phage-loaded Rh nanoparticle catalyst was prepared.

Embodiment 3

[0037] 1. Proliferation and enrichment of T4 phage

[0038] See step 1 of Embodiment 1, the process is exactly the same.

[0039] 2. Preparation of T4 phage-loaded Pd nanoparticles catalyst

[0040] Add 200 μL of the enriched T4 phage suspension to 200 μL of 1 mM PdCl 2 Mix well in the solution, and incubate at 4°C for 10 hours on a shaker at 60 r / min. Afterwards, ultracentrifuge at 4°C, 45000r / min for 3h, collect the obtained precipitate, and disperse in 200μL deionized water. Then add 60 μL freshly prepared 5 mM NaBH dropwise 4 solution, that is, the T4 phage-loaded Pd nanoparticle catalyst was prepared.

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Abstract

The invention discloses a preparation method of a T4 bacteriophage supported precious metal nano-particle catalyst. In the method, by utilizing inherent structural characteristics and molecular recognition function of living organisms, the precious metal nano-particle catalyst which is configured regularly on the capsid surface of a T4 bacteriophage and has uniform particles and high dispersion can be obtained through the steps of adding the T4 bacteriophage obtained by enrichment into a precious metal salt solution, hatching, centrifuging, and reducing. The method can control the size and the distribution of the precious metal nano-particles in a highly efficient mode, and greatly improves the electro-catalysis activity of the precious metal nano-particles. Meanwhile, by directly taking a biological nano-structure existing in the nature as a carrier, the method avoids complex preparation process of conventional carriers, thereby reducing production cost. Because the biological carriers have the characteristics of self-production, high feature repeatability and the like, the method is easy to realize large-scale production.

Description

technical field [0001] The invention belongs to the technical field of catalysis, and in particular relates to a preparation method of a T4 bacteriophage-loaded monodisperse noble metal nanoparticle catalyst. Background technique [0002] Noble metal platinum (Pt), rhodium (Rh), palladium (Pd) nanoparticles have high catalytic selectivity and high catalytic activity due to their small particle size, high surface activity and large specific surface area. More and more people pay attention to it, and it is gradually being used to replace nickel, copper, iron, etc., which are catalytic hydrogenation catalysts in traditional industries. Precious metal catalysts can also be used to eliminate carbon monoxide, hydrocarbons, and nitrogen oxides in automobile exhaust, and are currently popularly researched "three-way catalysts." Accompanied by the rapid growth of precious metal catalysts in industrial usage, the shortage of precious metal resources and the sharp fluctuations and con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/38B01J23/42B01J23/46B01J23/44B01J32/00B01J35/02B01D53/86B01D53/62
Inventor 高发明侯莉苏静静李娜赵新美
Owner YANSHAN UNIV
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