Preparation method for polyaniline-polyphenol oxidase sensor

A polyphenol oxidase and polyaniline technology, applied in the field of biosensors, can solve the problems of low charge transfer efficiency, low load capacity, poor conductivity, etc., and achieve good biocompatibility, fast response, and good stability

Inactive Publication Date: 2010-06-16
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The early "one-step method" was mostly carried out in a neutral medium. Under this condition, the polyaniline membrane synthesized had a compact structure, poor conductivity, low enzyme loading, and low charge transfer efficiency. neglected

Method used

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  • Preparation method for polyaniline-polyphenol oxidase sensor
  • Preparation method for polyaniline-polyphenol oxidase sensor
  • Preparation method for polyaniline-polyphenol oxidase sensor

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Embodiment Construction

[0017] 1. Preparation process:

[0018] 1. Raw materials:

[0019] Aniline (distilled under reduced pressure to colorless and stored in a refrigerator at 4°C for later use).

[0020] Polyphenol oxidase from mushrooms.

[0021] Ethyl sulfate-1-methyl-3-ethylimidazolium ionic liquid (according to literature J.D.Holbrey, W.M.Reichert, R.P.Swatlosk, G.A.Broker, W.R.Pitner, K.R.Seddon, R.D.Rogers, Green Chem., 4(2002) 407 method of preparation).

[0022] The remaining raw materials were of analytical grade.

[0023] All solutions were prepared with twice distilled water.

[0024] 2. Electrolyte composition:

[0025] Aniline: 0.1-0.5mol dm of the total electrolyte -3 .

[0026] Ethyl sulfate-1-methyl-3-ethylimidazolium ionic liquid: accounting for 5-50% of the total volume of the electrolyte.

[0027] Polyphenol oxidase: account for 0.1-1.0g dm of the total electrolyte -3 .

[0028] Hydrochloric acid: 0.1-3.0mol dm of the total electrolyte -3 .

[0029] Sodium dodecyl su...

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Abstract

The invention provides a preparation method for a polyaniline-polyphenol oxidase sensor, relates to the technical field of biosensors, and directly prepares the polyaniline-polyphenol oxidase sensor by direct electrochemical polymerization. The method comprises a step of immobilizing polyphenol oxidase in aniline solution containing ionic liquid and a surfactant to form the polyaniline-polyphenol oxidase sensor. The method mixes the enzyme, a monomer and supporting electrolyte solution by a 'one-step method'' and realizes the immobilization of the enzyme while electrolyzing on an electrode to form a conducting high polymer membrane. The method of the invention has the advantages of high bio-compatibility, proper pore structure and the like, and can realize the effective immobilization of the polyphenol oxidase. The polyaniline-polyphenol oxidase sensor prepared by the method has the advantages of high electrochemical activity even at a high pH, quick response to a substrate, high sensitivity, relatively wide linear range, and high repeatability and stability. The sensor can monitor phenolic compounds.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to the technical field of preparing polyaniline-polyphenol oxidase biosensors. Background technique [0002] Phenolic compounds are harmful to humans, animals and plants and have caused serious environmental problems. Therefore, the monitoring of phenolic compounds has become an important research topic. Polyphenol oxidase biosensors are widely used in the detection of phenolic compounds due to their high sensitivity, fast response, simple operation and easy miniaturization. The immobilization of enzymes is a key step in the construction of biosensors, and the materials and methods of immobilizing enzymes have an important impact on the performance of the sensors. At present, many materials such as magnetic nanoparticles, sol-gel, self-assembled monolayer, clay, etc. are used for the immobilization of polyphenol oxidase. However, some materials are complicated or time-consumin...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/413
Inventor 阚锦晴谭永艳
Owner YANGZHOU UNIV
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