Method for measuring concentration of cetirizine in human urine

A cetirizine and urine drug concentration technology, applied in the field of medical testing, can solve the problems of high price, cumbersome operation, time-consuming and laborious, etc., and achieve the effect of high precision and easy operation

Inactive Publication Date: 2010-06-30
曹翠珍
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  • Application Information

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Problems solved by technology

By measuring the concentration of cetirizine prototype drug in urine, its excretion pathway can be effectively studied. At present, the concentration determination method of cetirizine prototype drug in urine at home and abroad mainly adopts high performance liquid chromatography. It is cumbersome, time-consuming, labor-intensive, low efficiency, long measurement period, expensive instruments or reagents, etc. It is not the same as the monitoring of the excretion pathway of conventional therapeutic drugs.

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  • Method for measuring concentration of cetirizine in human urine
  • Method for measuring concentration of cetirizine in human urine
  • Method for measuring concentration of cetirizine in human urine

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[0013] 1. Instruments and reagents:

[0014] instrument:

[0015] Waters high-performance liquid chromatography: 510 pumps, Waters486 ultraviolet detector, Maxima 820 chromatographic workstation.

[0016] Reagent:

[0017] Cetirizine hydrochloride standard substance is provided by Jiangsu Lianyungang Pharmaceutical Factory; Xiantemin (Zyrtec) coated tablet (every piece of cetirizine hydrochloride 10mg, batch number 96A26 / A) is produced by Belgian UCB company; (Decloxizine Hydrochloride) was provided by the Pharmacy Department of Changhai Hospital Affiliated to Second Military Medical University; acetonitrile was chromatographic grade 1, sodium dihydrogen phosphate, sodium hydroxide, hydrochloric acid, triethylamine, SDS, etc. were all analytically pure, and water was double distilled water.

[0018] 2. Methods and Results

[0019] Chromatographic conditions and system suitability test: Chromatographic conditions Waters Nova-Pak C 18 (150×3.9mm, 4μm) chromatographic column;...

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Abstract

The invention relates to a method for analyzing and measuring the concentration of medicine in excrement, in particular to a method for measuring concentration of cetirizine in human urine, which belongs to the field of medical examination. The method comprises the following steps: after pretreating the sample, applying a high performance liquid chromatograph for detection; the chromatographic column is Waters Nova-Pak C18, the mobile phase is acetonitrile-0.02mol.L-1 sodium dihydrogen phosphate-triethylamine (50:50:0.15), the pH value is 3.07, 0.0105 mol.L-1 of sodium dodecyl sulfate (SDS) is contained, and the flow rate is 1.0mL/min; the column temperature is the room temperature; the internal standard is decloxizine, and the detection wavelength is 229nm. The method has the advantages of high accuracy and preciseness, and simple and rapid operation. The result proves that cetirizine is manly excreted from the kidney, therefore, medicines influencing the secretion of renal tubules possibly affect the excretion of cetirizine from the kidney, and attention is expected to be given to the mutual interaction between medicines when medicines are taken in a combined way.

Description

technical field [0001] The invention belongs to the field of medical examination, and relates to a method for analyzing and measuring drug concentration in excreta, in particular to a method for measuring the concentration of cetirizine in urine. Background technique [0002] Cetirizine is a highly selective second-generation histamine H 1 Receptor blockers have no obvious central inhibitory effect, and are mainly used clinically to prevent and treat allergic rhinitis (seasonal and non-seasonal), chronic idiopathic urticaria, allergic asthma, atopic dermatitis and other diseases. By measuring the concentration of cetirizine prototype drug in urine, its excretion pathway can be effectively studied. At present, the concentration determination method of cetirizine prototype drug in urine at home and abroad mainly adopts high performance liquid chromatography. It is cumbersome, time-consuming, labor-intensive, low efficiency, long measurement period, expensive instruments or re...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 曹翠珍
Owner 曹翠珍
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