Method for controlling plant virus harm by improving plant immunity and application thereof
A virus and purpose technology, applied in the fields of botanical equipment and methods, pest control, chemicals for biological control, etc., can solve problems such as not being able to achieve ideals, and achieve the goal of enhancing resistance to the invasion of plant viruses and improving immunity. Effect
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Embodiment 1
[0018] Using NK-F001, NK-F002, NK-F003 and SZG-7 etc. to improve the method and application of tobacco immunity against TMV
[0019] The in vitro direct antiviral activity was measured using the half-leaf method. The in vivo induction was to use common tobacco with the same seedling age, 3 pots as a group, and treat the tobacco seedlings that had been treated 7 days before inoculation respectively. The treatment methods included: spraying for supplying Test compound solution 2 to 3 times, 10 ml each time, or soil treatment, 10 ml each time, on the 7th day, inoculate TMV on the newly grown tobacco leaves by rubbing, and place the tobacco seedlings under the suitable temperature and light for 3 Two days later, check the onset situation, and the number of integrated lesions is calculated according to the formula for the induced antiviral effect of the test compound on TMV. Each treatment is repeated 3 times, and the control is divided into two kinds of blank control and standard d...
Embodiment 2
[0030]NK-F001, NK-F002, NK-F003 and SZG-7 were used to induce changes in the activity of phenylalanine ammonia-lyase (PAL) in tobacco after challenge inoculation with TMV
[0031] Get newly grown and inoculated TMV tobacco leaves to be tested after being induced by the above compounds, add 3 milliliters of 0.2 mol / liter borax buffer (pH8.8, containing 0.005 mol / liter of mercaptoethanol, 0.001 mol / liter of EDTA) and Polyvinylpyrrolidone (PVP), about 1 / 10 of the sample weight, was homogenized in an ice bath. Centrifuge at 10,000 rpm for 20 minutes at 4°C, and use the supernatant for enzyme activity determination. The assay system consisted of 2 mL of 0.2 mol / L borax buffer at pH 8.8. 1 ml of 0.02 mol / L L-phenylalanine and 0.8 ml of enzyme solution. After measuring OD290, react in a 37-degree water bath for 1 hour, and then measure OD290 value. The control is 3 ml of borax buffer solution plus 0.8 ml of enzyme solution. The protein content was determined by the Cowes Brilliant...
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