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Method for controlling plant virus harm by improving plant immunity and application thereof

A virus and purpose technology, applied in the fields of botanical equipment and methods, pest control, chemicals for biological control, etc., can solve problems such as not being able to achieve ideals, and achieve the goal of enhancing resistance to the invasion of plant viruses and improving immunity. Effect

Inactive Publication Date: 2010-07-07
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant virus disease is the "cancer" of plants, and its prevention and control methods mainly include physical methods and chemical methods-mechanical methods, but each method can not achieve the desired effect. In chemical methods, there is currently no one that is completely effective. Agents that can control plant virus diseases. Since the occurrence of viral diseases is closely related to the host plant, it has always been the ideal and direction of efforts for agricultural scientists to explore the mechanism of plant resistance to prevent and control viral diseases. , the plant activator is the outstanding achievement of research in this direction. It can also avoid the adverse effects of traditional pesticides on beneficial organisms by directly acting on pests, weeds and pathogens to achieve the purpose of insect control, weed control and disease prevention. And to the pressure of environment, at present the plant activator BTH that develops most successfully has been used widely in the world (Bakulev V A, Dehaen W.The Chemistry of 1,2,3-thiadiazole, 2004, John Wiley & Sons, Inc.) , the inventor started from basic research and was funded by the National Natural Science Foundation of China (30270883 and 20672062), the "973" Program (2003CB114402), the Tianjin Natural Science Foundation (07JCYBJC01200) and the Tianjin Science and Technology Support Program International Science and Technology Cooperation Project (07ZCGHHZ01400) Under the design, synthesis and discovery of N-(5-methyl-1,3-thiazol-2-yl)-4-methyl-1,2,3-thiadiazole-5-carboxamide (hereinafter referred to as thiadiazole-5-carboxamide) ) has good biological activity (Fan Zhijin et al., novel [1,2,3] thiadiazole derivatives and their synthesis method and application, National Invention Patent of the People’s Republic of China, Publication No.: CN1810808A; Fan Zhijin et al., have induced gold wire A new compound with secondary metabolic activity of plants of the genus Peach, National Invention Patent of the People's Republic of China, Publication No.: CN2020667A); Afterwards, the inventor carried out systematic structure optimization and creation research on this type of compound (Fan Zhijin, etc., Thiadiazole Formamide derivatives and their synthesis methods and uses, National Invention Patent of the People's Republic of China, application number: 200810052546.6; No.: 200810052547.0; Fan Zhijin, et al., Thiadiazole imine derivatives and their synthesis methods and uses, National Invention Patent of the People's Republic of China, application number: 200810052553.6)

Method used

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  • Method for controlling plant virus harm by improving plant immunity and application thereof
  • Method for controlling plant virus harm by improving plant immunity and application thereof
  • Method for controlling plant virus harm by improving plant immunity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Using NK-F001, NK-F002, NK-F003 and SZG-7 etc. to improve the method and application of tobacco immunity against TMV

[0019] The in vitro direct antiviral activity was measured using the half-leaf method. The in vivo induction was to use common tobacco with the same seedling age, 3 pots as a group, and treat the tobacco seedlings that had been treated 7 days before inoculation respectively. The treatment methods included: spraying for supplying Test compound solution 2 to 3 times, 10 ml each time, or soil treatment, 10 ml each time, on the 7th day, inoculate TMV on the newly grown tobacco leaves by rubbing, and place the tobacco seedlings under the suitable temperature and light for 3 Two days later, check the onset situation, and the number of integrated lesions is calculated according to the formula for the induced antiviral effect of the test compound on TMV. Each treatment is repeated 3 times, and the control is divided into two kinds of blank control and standard d...

Embodiment 2

[0030]NK-F001, NK-F002, NK-F003 and SZG-7 were used to induce changes in the activity of phenylalanine ammonia-lyase (PAL) in tobacco after challenge inoculation with TMV

[0031] Get newly grown and inoculated TMV tobacco leaves to be tested after being induced by the above compounds, add 3 milliliters of 0.2 mol / liter borax buffer (pH8.8, containing 0.005 mol / liter of mercaptoethanol, 0.001 mol / liter of EDTA) and Polyvinylpyrrolidone (PVP), about 1 / 10 of the sample weight, was homogenized in an ice bath. Centrifuge at 10,000 rpm for 20 minutes at 4°C, and use the supernatant for enzyme activity determination. The assay system consisted of 2 mL of 0.2 mol / L borax buffer at pH 8.8. 1 ml of 0.02 mol / L L-phenylalanine and 0.8 ml of enzyme solution. After measuring OD290, react in a 37-degree water bath for 1 hour, and then measure OD290 value. The control is 3 ml of borax buffer solution plus 0.8 ml of enzyme solution. The protein content was determined by the Cowes Brilliant...

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Abstract

The invention provides a method for inducing the immune system of plants by utilizing 4-methyl-1,23-thiadiazole-5-formic acid (NK-F001), 4-methyl-1,23-thiadiazole-5-formic acid (NK-F002), 4-methyl-1,23-thiadiazole-5-formic acid (NK-F003) and methyl thiadiazole formamide (SZG-7) to enable the plants to generate plant virus resistance to protect the plants. The invention also provides the application of the combined use of any two or three of 4-methyl-1,23-thiadiazole-5-formic acid (NK-F001), 4-methyl-1,23-thiadiazole-5-formic acid (NK-F002) and 4-methyl-1,23-thiadiazole-5-formic acid (NK-F003), methyl thiadiazole formamide (SZG-7) and diazosulfide (active ester, BTH), Tiadinil (TDL) and DL-beta-propalanine, virazole, ningnanmycin, antofine, virus satellite No.1 and virus satellite No.2 and XY-13 and XY-30 to improve the plant immunity to induce the plants to generate antiviral activity and processed dosage forms of the single preparations and the mixtures and the composition and the content of effective components in the dosage forms. The invention discloses the range of the plants which can be induced by the compounds and composites thereof, the antivirus range of the induced plants, and the application of the compounds and composites thereof in protecting the plants in the field of agricultural field and horticultural filed.

Description

technical field [0001] The technical scheme of the invention relates to a method for improving plant immunity by using chemically synthesized compounds, and the use of these compounds for improving the resistance of agricultural plants and horticultural plants to infection by virus diseases. Background technique [0002] Plant virus disease is the "cancer" of plants, and its prevention and control methods mainly include physical methods and chemical methods-mechanical methods, but each method can not achieve the desired effect. In chemical methods, there is currently no one that is completely effective. Agents that can control plant virus diseases. Since the occurrence of viral diseases is closely related to the host plant, it has always been the ideal and direction of efforts for agricultural scientists to explore the mechanism of plant resistance to prevent and control viral diseases. , the plant activator is the outstanding achievement of research in this direction. It ca...

Claims

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Application Information

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IPC IPC(8): A01N43/828A01N47/28A01N43/653A01N37/44A01P1/00A01P21/00
Inventor 范志金张海科马琳范志银左翔米娜
Owner NANKAI UNIV
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