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In vitro conservation method of oriental hybrid lily germ plasm resource

An in vitro preservation and germplasm technology, applied in horticultural methods, plant preservation, botanical equipment and methods, etc., can solve the problems of easy differentiation of leaves from outer scales, unfavorable accumulation of nutrients in bulbs, and easy necrosis of bulb tissues. To control the growth of callus, improve the efficiency of in vitro preservation, and save labor

Inactive Publication Date: 2010-08-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to aim at the existing defects in the in vitro preservation method of lily germplasm resources that the bulb tissue is easily necrotic, and the outer scales are easy to differentiate into leaves, aging, withered, unfavorable for bulb nutrient accumulation, and short shelf life. , proposed a method for preservation of germplasm resources of oriental lily with good quality, long storage period, self-adjustment and extension, low cost, easy operation, and suitable for in vitro preservation of oriental lily germplasm resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: (in vitro preservation of oriental lily germplasm resources 1)

[0033] The specific method is as follows:

[0034] (1) The preparation of the culture medium: including the basic medium and the components of the medium for each stage of in vitro storage and the weight of each component in each liter of medium:

[0035] a) basic medium: wherein,

[0036] Induction medium is sucrose 40g / L, agar powder 4g / L, MS medium with pH5.8;

[0037] Proliferation medium is 50g / L sucrose, 5g / L agar powder, 3 / 5MS medium with pH5.6;

[0038] The medium for in vitro preservation and tube bulb expansion is 1 / 2MS medium with 60g / L sucrose, 5.0g / L agar powder, and pH5.6;

[0039] b) Induction medium I: MS+6-BA 1.0mg / L+IBA 0.1mg / L+AC 2.0mg / L;

[0040] c) Induction medium II: MS+6-BA 1.0mg / L+IBA 0.25mg / L;

[0041] d) Proliferation medium: 3 / 5MS+6-BA1.0mg / L+IBA0.5mg / L;

[0042] e) Synchronous culture medium for in vitro preservation and tube bulb expansion: 1 / 2MS+NAA 0.1mg / ...

Embodiment 2

[0050] Embodiment 2: (in vitro preservation of oriental lily germplasm resources 2)

[0051] In this example, the preparation of step (1) medium is:

[0052] a) basic medium: wherein,

[0053] Induction medium is 30g / L sucrose, 4.5g / L agar powder, MS medium with pH5.6;

[0054] Proliferation medium is 55g / L sucrose, 4.5g / L agar powder, 3 / 5MS medium with pH5.7;

[0055] The medium for in vitro preservation and tube bulb expansion is 1 / 2MS medium with 68g / L sucrose, 4.5g / L agar powder, and pH5.8;

[0056] b) Induction medium I: MS+6-BA 2.0mg / L+IBA 0.5mg / L+AC 1.0mg / L;

[0057] c) Induction medium II: MS+6-BA 0.75mg / L+IBA 0.1mg / L;

[0058] d) Proliferation medium: 3 / 5MS+6-BA0.2mg / L+IBA0.1mg / L;

[0059] e) Synchronous culture medium for in vitro preservation and tube bulb expansion: 1 / 2MS+NAA 0.5mg / L+PP333 5mg / L+mannitol 5.0g / L+AC 5.0mg / L;

[0060] Step (2) Provenance Bulb Treatment and Sterilization: Put 50 DEG C of incubator heat treatment 80min; Its sterilization treatment...

Embodiment 3

[0061] Embodiment 3: (in vitro preservation method 3 of oriental lily germplasm resources)

[0062] In this example, the preparation of step (1) medium is:

[0063] a) basic medium: wherein,

[0064] Induction medium is sucrose 50g / L, agar powder 5.0g / L, pH5.7 MS medium;

[0065] Proliferation medium is 3 / 5MS medium with sucrose 60g / L, agar powder 4.0g / L, pH5.8;

[0066] The medium for in vitro preservation and tube bulb expansion is 1 / 2MS medium with 75g / L sucrose, 4.0g / L agar powder, and pH6.0;

[0067] b) Induction medium I: MS+6-BA 1.5mg / L+IBA 0.3mg / L+AC 3.0mg / L;

[0068] c) Induction medium II: MS+6-BA 0.5mg / L+IBA 0.5mg / L;

[0069] d) Proliferation medium: 3 / 5MS+6-BA0.5mg / L+IBA0.3mg / L;

[0070] e) Synchronous culture medium for in vitro preservation and tube bulb expansion: 1 / 2MS+NAA 0.3mg / L+PP333 10mg / L+mannitol 3.0g / L+AC 3.0mg / L;

[0071] Step (2) Provenance Bulb Treatment and Sterilization: Heat treatment in 50°C incubator for 90 minutes; its sterilization treatm...

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Abstract

The invention discloses an in vitro conservation method of an oriental hybrid lily germ plasm resource, belonging to the technical field of plant tissue culture and germ plasm resource conservation. The in vitro conservation method of the oriental lily germ plasm resource comprises the following steps of: preparing a culture medium; processing and sterilizing provenance bulbs; carrying out induction culture of bulb buds; carrying out propagation culture of the bulb buds; carrying out in vitro conservation and expansion culture of germ plasms; sprouting, refrigerating and planting in-vitro bulbs; detecting the genetic stability of the germ plasms, and the like. By improving the formula of the culture medium and the culture steps and method, the invention prolongs a transferring-culture period of the in vitro conservation to 12-15 months, more than 6 months longer than that of an MS culture medium, achieves high survival rate of 100 percent without generating genetic variation by detection, enhances the efficiency of the in vitro conservation, also obviously reduces the conservation cost and can be popularized in and applied to oriental hybrid lily breeding and seedball producing enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation and germplasm resource preservation, in particular to an in vitro preservation method for prolonging oriental lily germplasm. Background technique [0002] Oriental hybrid lily (Oriental hybrid lily) is a perennial ornamental plant, which is mainly used for the cultivation of cut flowers. In recent years, the production of cut lily in my country has increased by more than 20% annually. Due to the beautiful shape of the plant, the quality of cut flowers, the resistance to gray mold, the insensitivity to photoperiod, and the good resistance to cold storage of the bulbs, in my country, we can make use of the climate differences at different latitudes and altitudes and combine facility cultivation to realize lily cut flowers. Annual production, high production efficiency, so it has a good market development prospect. At present, my country's oriental lily bulbs mainly re...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01N3/00A01G31/00
Inventor 郭方其丁渭文黎侠孙崇波向林柴金甫林森洪袁峰丁晓瑜贾燕娇
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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