Preparation method of ligustrum lucidum polysaccharide

A technology of privet fruit polysaccharide and ultrafiltration membrane, which is applied in the direction of anti-toxic agent, drug combination, ultrafiltration, etc., can solve the problems of many types of organic solvents, loss of active ingredients, high toxicity, etc., and achieve good crystallization effect, low toxicity, The effect of production safety

Inactive Publication Date: 2010-08-18
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Degreasing uses petroleum ether, which is large in dosage and highly toxic; there are many types of organic solvents used in the process, which may easily lead to the loss of active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 1kg of the residue of triterpene acid extracted from Ligustrum lucidum, add 3L of warm water at 30°C each time for soaking, repeat squeezing twice at 80Mpa, and combine the squeezed liquid. The squeezed liquid was concentrated into 282g of extract, and the extract was washed successively with 1.2L, 850ml, and 850ml of absolute ethanol. The residual solid was dispersed with 3 L of deionized water, and 600 ml of chloroform:n-butanol=5:1 (V / V) mixture was added to remove protein by Sevage method. Finally, the remaining solution was concentrated through a polyalum hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 20,000, and dried to obtain 3.8 g of Ligustrum lucidum polysaccharide, which was detected by HPLC with a purity of 98.5%.

Embodiment 2

[0024] Take 1kg of the residue of triterpene acid extracted from Ligustrum lucidum, add 4L of warm water at 40°C each time to soak, squeeze twice at 90Mpa, and combine the squeezed liquid. The squeezed liquid was concentrated into 294g of extract, and the extract was washed successively with 1.5L, 1.5L, and 900ml of absolute ethanol. The residual solid was dispersed with 3 L of deionized water, and 600 ml of chloroform:n-butanol=5:1 (V / V) mixture was added to remove protein by Sevage method. Finally, the remaining solution was concentrated through a polyalum hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 20,000, and dried to obtain 3.9 g of Ligustrum lucidum polysaccharide, which was detected by HPLC with a purity of 98.6%.

Embodiment 3

[0026] Take 1kg of the residue of triterpene acid extracted from Ligustrum lucidum, soak in 5L of 40°C warm water each time, squeeze repeatedly at 100Mpa for 3 times, and combine the squeeze liquid. The squeezed liquid was concentrated into 302g of extract, and the extract was washed successively with 1.5L, 1.2L, and 1.2L of absolute ethanol. The residual solid was dispersed with 3 L of deionized water, and 600 ml of chloroform:n-butanol=5:1 (V / V) mixture was added to remove protein by Sevage method. Finally, the remaining solution was concentrated through a polyalum hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 20,000, and dried to obtain 3.8 g of Ligustrum lucidum polysaccharide, which was detected by HPLC with a purity of 99.2%.

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Abstract

The invention relates to a preparation method of ligustrum lucidum polysaccharide; the process takes dry residue after ligustrum lucidum extracts triterpenoid as the raw materials, soaked in 30 to 50DEG C warm water with 3 to 5 times of amount every time (V/M) for 1 to 2h, repeatedly pressed for 2 to 3 times; the combined pressed liquid is concentrated into extract at low pressure and washed by absolute ethyl alcohol for 3 times, to prepare solid deionized water to be re-dissolved; and protein is removed through a Sevage method, deproteinized solution is concentrated by an ultrafilter film, to prepare the product. The process has the advantages of simple operation, lower cost, higher purity and facilitation to mass production operation.

Description

technical field [0001] The invention relates to a preparation method of Ligustrum lucidum polysaccharide, in particular to a method for extracting and purifying Ligustrum lucidum polysaccharide by using squeezing, Sevage method and ultrafiltration membrane technology. Background technique [0002] Ligustrum polysaccharide is one of the material bases of Ligustrum lucidum with multiple functions. Modern pharmacological studies have shown that Ligustrum lucidum polysaccharide has anti-oxidation, anti-aging, and immune-enhancing effects. It has been identified that it is mainly composed of rhamnose, arabinose, glucose and Fucose is composed of four sugars with a molecular weight between 2 and 1 million. [0003] Rhamnose is a derivative in which a hydroxyl group at the 6-position of mannose is replaced by hydrogen, that is, 6-deoxy-L-mannose. Also known as methyl pentose. One of the typical 6-deoxyhexoses juxtaposed with fucose. It is the 6th deoxygenation of mannose, and fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00B01D61/14A61P39/06A61P37/04
Inventor 李法庆张翼刘东锋
Owner 苏州宝泽堂医药科技有限公司
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