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Extraction and purification method of cerasus humilis red pigment

A purification method and red pigment technology, applied in the field of Oli, can solve the problems of extraction process, solvent residue, low purity, etc., and achieve the effect of shortening the extraction time, increasing the yield, and good color

Inactive Publication Date: 2010-08-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the traditional extraction method, there are disadvantages such as solvent residue, low purity, peculiar smell, and poor quality. Since pomegranate belongs to anthocyanin, it is unstable under neutral and weak alkaline conditions, which brings great problems to the extraction process. troubled
Pigment purification is not enough, the coloring ability is not strong, and the stability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Raw material selection and beating of Ou Li: select fresh 8-9 mature, non-rotten and non-pesticide-polluted Ou Li fresh fruits, cleaned, pitted and beaten.

[0016] (2) Ultrasonic extraction at constant temperature: mix the Plum syringum slurry with 80% ethanol (v / v) at a mass ratio of 1:5, adjust the pH value of the mixture to 3.0 with citric acid, set the ultrasonic power at 500w, temperature at 40°C, and ultrasonic time Extract under the condition of 20min, filter the extract with a 150-mesh filter, wash the residue with an acidic ethanol solution with a pH value of 3.0, centrifuge the filtrate at 3000r / min for 20min, and take the supernatant.

[0017] (3) Purification by ultrafiltration: the supernatant was ultrafiltered with a 0.1 μm ultrafiltration membrane under the conditions of a feed temperature of 30° C. and a feed pressure of 0.3 MPa, and was concentrated by a rotary evaporator to obtain a concentrated solution of Prunus red pigment.

[0018] (4) Macropo...

Embodiment 2

[0021] (1) Raw material selection and beating of Ou Li: select fresh 8-9 mature, non-rotten and non-pesticide-polluted Ou Li fresh fruits, cleaned, pitted and beaten.

[0022] (2) Ultrasonic extraction at constant temperature: mix the Plum syringum slurry with 80% ethanol (v / v) at a mass ratio of 1:4, adjust the pH value of the mixture to 2.0 with citric acid, set the ultrasonic power to 100w, the temperature at 25°C, and the ultrasonic time Extract under the condition of 60min, filter the extract with a 150-mesh filter, wash the residue with an acidic ethanol solution with a pH value of 2.0, centrifuge the filtrate at 3000r / min for 20min, and take the supernatant.

[0023] (3) Purification by ultrafiltration: the supernatant was ultrafiltered with a 0.15 μm ultrafiltration membrane under the conditions of a feed temperature of 40° C. and a feed pressure of 0.2 MPa, and was concentrated by a rotary evaporator to obtain a concentrated solution of Prunus red pigment.

[0024] (4...

Embodiment 3

[0027] (1) Raw material selection and beating of Ou Li: select fresh 8-9 mature, non-rotten and non-pesticide-polluted Ou Li fresh fruits, cleaned, pitted and beaten.

[0028] (2) Ultrasonic extraction at constant temperature: mix the Prunus japonica slurry with 80% ethanol (v / v) at a mass ratio of 1:6, adjust the pH value of the mixture to 3.5 with citric acid, and set the ultrasonic power at 1000w, temperature at 50°C, and ultrasonic time Extract under the condition of 10min, filter the extract with a 150-mesh filter, wash the residue with an acidic ethanol solution with a pH value of 3.5, centrifuge the filtrate at 3000r / min for 20min, and take the supernatant.

[0029] (3) Purification by ultrafiltration: the supernatant was ultrafiltered with a 0.2 μm ultrafiltration membrane under the conditions of a feed temperature of 50° C. and a feed pressure of 0.1 MPa, and concentrated by a rotary evaporator to obtain a concentrated liquid of Prunus red pigment.

[0030] (4) Macrop...

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PUM

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Abstract

The invention relates to an extraction and purification method of cerasus humilis red pigment, which comprises the following steps that: cerasus humilis serum is mixed with 80 percent ethanol by 1: (4-6), the pH of the mixture is regulated to 2.0 to 3.5, the mixture is extracted when the ultrasonic power is 100 to 1000w and the temperature is 25 to 50DEG C, the extract is filtered by a 150-mesh filter mesh, acid ethanol solution is used to wash residue, and the filtrate is centrifuged to prepare supernatant; the supernatant is ultrafiltered by a 0.1 to 0.2 mu m ultrafiltration film when the feeding temperature is 30 to 50DEG C and the pressure is 0.1 to 0.3 MPa, and is concentrated to prepare concentrate; Amberlite XAD-7HP macroporous resin is used to absorb the concentrate, 70 percent ethanol is used to elute, and the flowing speed is 2.0 to 4.0mL / min; the solution absorbed by the macroporous resin is concentrated, frozen in a refrigerator for 6h, frozen and dried under a vacuum condition for 10h to prepare the cerasus humilis red pigment; and the method has high cerasus humilis red pigment extraction rate, high purity and good color.

Description

technical field [0001] The invention relates to a red pigment extraction and purification method of Prunus humilis Bunge, also known as Prunus humilis Bunge. Background technique [0002] Plum is a small perennial deciduous shrub belonging to the family Rosaceae. It is the smallest woody fruit tree in the world unique to my country. The root-to-shoot ratio of the fruit tree is 9:1. It can fix sand and store water and other six characteristics. Prune pigment is the main pigment of mature Plum, mainly red, with bright color and strong aroma. It is not only a good product for fresh food, but also can be used to extract natural food coloring or spices. [0003] In recent years, the research and development of natural pigments in my country has gradually matured, but there are still some difficulties in the extraction of pigments. Using the traditional extraction method, there are disadvantages such as solvent residue, low purity, peculiar smell, and poor quality. Since pomegran...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
Inventor 周家华兰彦平常虹周连第刘淑琴冯翠萍田金强
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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