Method for extracting high-purity phospholipid from sleeve-fish-processing waste

Abstract

The invention, which belongs to the technical field of substance extraction, relates to a method for extracting high-purity phospholipid from sleeve-fish-processing waste. Fresh sleeve-fish-processing waste is first dried until the moisture content is less than or equal to 3 percent, and is ground to 10 to 80 meshes; the preprocessed powdery material is then loaded into an extraction kettle, and after the air is removed, extracting solvent CO2 is injected to set pressure; the supercritical extraction and separation of the extraction system is regulated as three processes, i.e. extraction, primary separation and secondary separation, raffinate is taken out of the extraction kettle after triglycercide and cholesterol are separated and removed, ethanol which is 4 to 10 times the weight of the raffinate is added in the raffinate according to a weight ratio to dissolve the raffinate, clear solution is taken out and concentrated, and is then washed by acetone, the obtained solid substance is dissolved in ethanol and then mixed with water, and precipitate which is obtained by centrifugation is dried, so that sleeve-fish phospholipid is obtained. The method has the advantages of less organic solvent dosage, little environment pollution, simple processing equipment, easy operation, automatic control, stable product quality, low production cost, good quality and high purity.

Description

Technical field: The invention belongs to the technical field of extraction and preparation of material components, and relates to a method for extracting high-purity phospholipids from squid processing waste, using supercritical CO 2 Extraction technology and equipment separate and extract the active ingredients in natural products and process them. Background technique: Phospholipids are lipids containing phosphate radicals, which are the basic components of tissue cells and have important biological functions. The highly unsaturated fatty acids contained in phospholipids are an important source of polyenoic acid in the human body; phospholipids can reduce the content of cholesterol triglycerides in the blood, improve lipid metabolism and arteriosclerosis; phospholipids are typical compatible molecules with relatively Good emulsifying function is the basic component of liposome and an important component of total intravenous nutrition emulsion. It has high medical value a...

AI Technical Summary

Problems solved by technology

At present, the supercritical fluid extraction method has been well applied in the extraction and preparation of terrestrial animal and plant phospholipids such as soybean phospholipids and egg yolk phospholipids, but it has not been used to extract and prepare marine animals, especially squid and their processing waste. Published reports on phospholipids

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