Special primer for aided-identifying strains of lactobacillus and application thereof

A Lactobacillus genus, milk identification technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high labor intensity and long time consumption
CN101818147AInactive Publication Date: 2010-09-01FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Publication Date
2010-09-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention claims a special primer for aided-identifying strains of lactobacillus and the application thereof. The special primer is composed of a primer pair A and a primer pair B; the primer pair A is composed of the DNA of the sequences 13 and 14 in the sequence table; and the primer pair B is composed of the DNA of the sequences 15 and 16 in the sequence table. The PCR amplification is carried out on the total DNA of the strain to be detected by using the special primer; if the PCR product can be amplified by the primer pair A or the primer pair B, the strain to be detected is the candidate strain of lactobacillus; if no PCR product is amplified by the primer pair A or the primer pair B, the strain to be detected is the candidate strain of non-lactobacillus. In combination with the denaturing gradient gel electrophoresis (DGGE) or the sequencing technology, the invention further determines that the strain to be detected is the specific strain of lactobacillus. By adopting the special primer of the invention and combining with the PCR and the DGGE, the planting condition of the probiotics on the intestinal wall of aquatic animal can be directly identified. The method is the fastest, simplest, most convenient and effective method at present.
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Description

technical field

[0001] The invention relates to a special primer for assisting identification of Lactobacillus strains and application thereof. Background technique

[0002] Whether it is in clinical medicine or in the prevention and control of livestock and poultry aquatic diseases, the infection of the intestinal tract by pathogenic bacteria is considered to be one of the ways to cause diseases. With the improvement of people's health awareness and the enhancement of drug resistance of pathogenic bacteria, biological therapy has gradually replaced traditional chemotherapy (such as applying antibiotics). Taking probiotics, such as Lactobacillus and Bifidobacterium, can effectively inhibit the growth of pathogenic bacteria in the intestine and benefit the intestinal microecological environment. However, to play a probiotic role, it is a necessary prerequisite to determine that probiotics can adhere tightly to intestinal mucosal epithelial cells. In some experiments, the re...

Claims

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