Quality detection method of melbine glipizide preparation
A technology of metformin glipizide and quality testing method, which is applied in the field of medicine, can solve the problems of inapplicable testing, adverse effects of patients, and not containing glipizide, etc., and achieves easy operation, strong specificity, and reproducibility Good results
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Embodiment 1 2
[0182] Embodiment 1 Determination of Related Substances of Metformin Glipizide Tablets
[0183] Glipizide 2.5g, Metformin Hydrochloride 250g, Microcrystalline Cellulose 149g, Povidone K 30 10g, sodium carboxymethyl starch 36g, magnesium stearate 2.5g are made into 1000 tablets according to the conventional process;
[0184] Glipizide related substances:
[0185] Determine according to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition two appendix V D).
[0186] Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler; ammonium dihydrogen phosphate solution (weigh 0.575g ammonium dihydrogen phosphate and 4.32g sodium octane sulfonate, add water to 1000ml, use three Ethylamine adjusted the pH to 7.0±0.05)-acetonitrile (84:16) as mobile phase; detection wavelength was 275nm; column temperature: 30°C.
[0187] Determination method Take an appropriate amount of fine powder of this product (approximately equi...
Embodiment 2 2
[0188] Example 2 Determination of Related Substances in Metformin Glipizide Capsules
[0189] Glipizide 2.5g, metformin hydrochloride 500g, microcrystalline cellulose 297g, povidone K 30 23.6g, sodium carboxymethyl starch 72g, magnesium stearate 4.9g were made into 1000 capsules according to the conventional process;
[0190] Glipizide related substances:
[0191] Determine according to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition two appendix V D).
[0192] Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler; ammonium dihydrogen phosphate solution (weigh 0.575g ammonium dihydrogen phosphate and 4.32g sodium octane sulfonate, add water to 1000ml, use three Ethylamine adjusted the pH to 7.0±0.05)-acetonitrile (84:16) as mobile phase; detection wavelength was 275nm; column temperature: 30°C.
[0193] Determination method Take an appropriate amount of fine powder of the content of this product (a...
Embodiment 3 2
[0197] Example 3 Determination of Related Substances in Metformin Glipizide Enteric-Coated Capsules
[0198] Glipizide 2.5g, metformin hydrochloride 500g, microcrystalline cellulose 297g, povidone K 30 23.6g, 72g of sodium carboxymethyl starch, and 4.9g of magnesium stearate are packed into enteric-coated capsule shells according to the conventional process, and made into 1000 capsules;
[0199] Determine according to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition two appendix V D).
[0200] Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler; ammonium dihydrogen phosphate solution (weigh 0.575g ammonium dihydrogen phosphate and 4.32g sodium octane sulfonate, add water to 1000ml, use three Ethylamine adjusted the pH to 7.0±0.05)-acetonitrile (84:16) as mobile phase; detection wavelength was 275nm; column temperature: 30°C.
[0201] Determination method Take an appropriate amount of fine powder of...
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