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Method for production of succinic acid

A technology of succinic acid and organic raw materials, applied in recombinant DNA technology, fermentation and other directions

Inactive Publication Date: 2010-09-01
MITSUBISHI CHEM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, Patent Document 2 discloses a method for producing succinic acid using a coryneform bacterium modified so that pyruvate carboxylase activity is enhanced, but there is no detailed explanation of the relationship between the reaction temperature of the bacterium and the succinic acid productivity. situation study

Method used

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  • Method for production of succinic acid
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  • Method for production of succinic acid

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preparation example Construction

[0054] 2. The preparation method of succinic acid

[0055] The method for producing succinic acid according to the present invention is characterized in that succinic acid is generated by reacting the bacterial cells obtained by culturing the above-mentioned bacteria or their processed product with the organic raw material in a reaction liquid containing the organic raw material, and collecting the succinic acid.

[0056] When the above-mentioned bacteria are used for the production of succinic acid, the bacteria cultured on a slant surface with a solid medium such as agar medium can be directly used for the reaction, but it is preferable to use the above-mentioned bacteria cultured in a liquid medium in advance (seed culture). obtained bacteria. As a medium used for seed culture, a common medium used for bacterial culture can be used. For example, a general medium obtained by adding natural nutrient sources such as meat extract, yeast extract, and peptone to a composition co...

Embodiment 1

[0088] Evaluation in Fermenter - 1

[0089]

[0090] 100mL culture medium (urea: 4g, ammonium sulfate: 14g, potassium dihydrogen phosphate: 0.5g, dipotassium hydrogen phosphate: 0.5g, magnesium sulfate heptahydrate: 0.5g, ferrous sulfate heptahydrate: 20mg, manganese sulfate Hydrate: 20mg, D-biotin: 200μg, thiamine hydrochloride: 200μg, yeast extract: 5g, casein hydrolyzate: 5g, and distilled water: 1000mL) into a 500mL Erlenmeyer flask, at 120°C Heat sterilize for 20 minutes. Cool it to room temperature, and add 4 mL of 50% glucose aqueous solution that has been sterilized in advance, inoculate the Brevibacterium flavum MJ233 / PC-4 / ΔLDH or MJ233 / ΔLDH constructed above, and seed at 30°C for 16 hours. to cultivate.

[0091]

[0092]Mix 365mL culture medium (ammonium sulfate: 1.0g, potassium dihydrogen phosphate: 1.5g, dipotassium hydrogen phosphate: 1.5g, potassium chloride: 1.67g, magnesium sulfate heptahydrate: 0.5g, ferrous sulfate heptahydrate: 40 mg, manganese sulfat...

Embodiment 2

[0100] Evaluation in Fermenter - 2

[0101] For the Brevibacterium flavum MJ233 / PC-4 / ΔLDH strain obtained in the same way as the seed culture of Example 1, under the temperature conditions of 35°C, 36°C, 37°C, 38°C and 39°C, follow the same method as in Example 1 Methods, evaluation of succinic acid production cultures was performed. The relationship between the accumulated concentration of succinic acid and the reaction temperature after 6 hours is as follows image 3 shown.

[0102] Depend on image 3 It can be seen that the succinic acid production efficiency of the MJ233 / PC-4 / ΔLDH strain increases rapidly from 37°C or higher.

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Abstract

Disclosed is a method for producing succinic acid, which is characterized by acting a cultured cell of a bacterium selected from Brevibacterium flavum, Brevibacterium lactofermentum and Corynebacterium glutamicum or a treatment product of the cultured cell on an organic raw material in a reaction solution containing the organic raw material to produce succinic acid and collecting succinic acid, wherein the bacterium is so modified as to have an enhanced pyruvate carboxylase activity compared with an unmodified one, and wherein the cultured cell or the treatment product thereof is acted on the organic raw material at 37 to 45 DEG C.

Description

technical field [0001] The present invention relates to methods for the production of succinic acid using microorganisms. Background technique [0002] A method for producing useful substances such as succinic acid using a genetically modified coryneform bacterium is known (see Patent Document 1 or 2). When coryneform bacteria are cultivated to produce succinic acid, the following method is often used. First, bacteria are grown under aerobic conditions to obtain bacterial cells, which are used as static bacterial cells and reacted with organic raw materials. In this case, generally, the growth of bacteria is carried out in the range of 25 to 35°C, and the reaction with the organic raw material is carried out in the range of 30 to 37°C. [0003] In Patent Document 3, regarding the lactate dehydrogenase-deficient strain of coryneform bacteria, the relationship between the growth temperature and the reaction temperature and the productivity of succinic acid was analyzed, and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N15/09
CPCC12P7/46
Inventor 村瀬诚米仓円佳城户大助青山龙介汤村秀一小池砂奈惠
Owner MITSUBISHI CHEM CORP
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