Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing casein antioxidant peptide by chymosin

A casein peptide and antioxidant peptide technology, applied in the biological field, can solve problems such as toxicity, carcinogenicity, and human health hazards, and achieve easy-to-implement, economical, convenient, and remarkable effects

Inactive Publication Date: 2010-09-08
DALIAN POLYTECHNIC UNIVERSITY
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antioxidants can be divided into natural antioxidants and synthetic antioxidants. Synthetic chemical antioxidants such as butyl hydroxyanisole and 2,6-di-tert-butyl-p-cresol are usually used as food additives in the food industry to prevent food Oxidative discoloration or quality deterioration caused by oxygen, but because synthetic antioxidants have certain toxicity and carcinogenic effects, there is a potential danger to human health, so safer natural antioxidants have become the focus of recent research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 12.5g of casein and dissolve it in 200mL of distilled water. Put it on an electromagnetic stirrer, and adjust the pH to 8.0 with 1mol sodium hydroxide after fully dissolving. Add the preheated enzyme preparation-rennet at a ratio of 2.5%. After the hydrolysis reaction time is 60 minutes, the enzyme is extinguished at 100°C for 15 minutes to stop the enzymatic hydrolysis reaction and generate casein hydrolyzate. Adjust the pH of the casein hydrolyzate to 4.6 to remove macromolecular substances in the enzymolyzate, and centrifuge at 3500r / min for 15min. The ultrafiltration membranes with a molecular weight cut-off of 3000 and 1000 were used for fractionation to obtain three groups of antioxidant peptide solutions.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing casein antioxidant peptide with higher free radical clearance rate based on chymosin specifity acting on casein. The product prepared by the invention has anticancer, anti-inducing and anti-aging bioactivities, is widely used in food, medicine and other industries, and is a promising basic material for health food development as an antioxidant of food and human body. The research suggests that antioxidant effect of casein peptide is that the highest clearance rate of DPPH- is 90.94% and the highest clearance rate of -OH is 73.33% under the condition of pH value of 8.0, temperature of 50 DEG C, E / S of 2.5%, S% of 6%, and hydrolyzation time of 60min.

Description

technical field [0001] The invention belongs to the field of biotechnology, and uses an enzymatic method to generate casein antioxidant peptides, specifically, uses rennet to generate casein antioxidant peptides. Background technique [0002] Studies have found that many human diseases such as myocardial infarction, arteriosclerosis, cancer, osteoporosis and obesity are related to oxidation. Therefore, the intake of substances with antioxidant activity can reduce the content of free radicals in the body, prevent the occurrence of oxidation reactions, and improve the body's resistance to diseases. Antioxidants can be divided into natural antioxidants and synthetic antioxidants. Synthetic chemical antioxidants such as butyl hydroxyanisole and 2,6-di-tert-butyl-p-cresol are usually used as food additives in the food industry to prevent food Oxidative discoloration or quality deterioration caused by oxygen, but because synthetic antioxidants have certain toxicity and carcinogen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 牟光庆陈东平王际辉钱方
Owner DALIAN POLYTECHNIC UNIVERSITY