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Sample adding device and application thereof

A sampling device and sampling area technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biological material sampling methods, etc., can solve problems such as pollution, difficult to eliminate contaminated initial samples, uneconomical, etc.

Inactive Publication Date: 2010-09-22
上海浩源生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the inefficiency, uneconomical and potential occurrence of more pollution (especially difficult to exclude Contamination and irreversible initial sample contamination, etc.), provide a sample loading device that integrates sample collection, nucleic acid extraction, and detection reagent sample loading functions

Method used

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  • Sample adding device and application thereof
  • Sample adding device and application thereof
  • Sample adding device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 sample loading device

[0055] Exemplary sample adding device comprises test tube, sample adding device housing, dispenser, stirring bar and magnetic rod, is characterized in that, comprises sample pooling area, nucleic acid extraction area, buffer zone, and test reagent in the described sample adding device housing The sampling area, where,

[0056] The sample collection area includes a dispenser positioned on the upper guide rail, and the lower layer includes: a sample liquid storage tank 11 including a plurality of test tubes 111 for storing sample liquid, a disposable pipette including a plurality of disposable pipettes 121 A storage tank 12, a pipette disposal container 13, a collection sample solution storage tank 14 comprising a plurality of test tubes 141, a reagent storage tank 16 comprising a plurality of test tubes 161 for at least storing nucleic acid lysate and a binding solution, and a test tube holder 151 A storage tank 15 for removable load...

Embodiment 2

[0061] Example 2 Sample addition method

[0062] According to the sample loading device described in Example 1, before adding samples, the doors are all in a closed state, and samples, reagents, lotions, magnetic beads, and eluents are all added to the test tubes on the corresponding storage tanks, wherein the The test tube rack 151 is loaded on the storage tank 15 of the removable load in the sample collection area, and the eluent test tube rack 251 is loaded on the storage tank 25 of the second movable load in the nucleic acid extraction area.

[0063] Performing a sample addition involves the following steps:

[0064](a1) In the sample collection area, the dispenser moves sequentially and inserts the disposable pipette stored in the disposable pipette storage tank 12, moves and sucks the sample solution stored in the sample solution storage tank 11, moves and Inject the sample solution into the test tube in the pooled sample solution storage tank 14, move and discard the d...

Embodiment 3

[0076] Example 3 Nucleic acid testing device

[0077] An exemplary nucleic acid testing device includes the sample loading device described in Example 1 and a PCR instrument, and the sample loading mixture storage tank 44 in the sample loading device is installed on the PCR instrument.

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Abstract

The invention provides a sample adding device which comprises a sample collecting area, a nucleic acid extracting area and a testing reagent sample adding area, integrates the functions of sample collection, nucleic acid extraction and testing reagent sample addition, and can be widely applied in automatic sample adding applications in sample analysis or testing in the fields of biology, immunology, medicine and the like, in particular to large-scale automatic analysis or screening. In addition, the invention further discloses a testing device comprising the sample adding device and a method for carrying out sample adding, analysis and testing.

Description

technical field [0001] The invention belongs to the technical field of biological testing. Specifically, the invention discloses a sampling device, which integrates the functions of sample collection, nucleic acid extraction, and detection reagent addition, and can be widely used in the fields of biology, immunology, medicine, etc. Automatic sampling application in sample analysis or testing, especially suitable for large-scale automatic analysis or screening. In addition, the invention also discloses a testing device including a sample adding device and a method for sample adding, analyzing or testing. Background technique [0002] In a large number of clinical experiments and laboratory experiments in the fields of biology, immunology, medicine, forensic science, etc., analysis of biological macromolecules (such as nucleic acids, proteins, etc.) based on polymerase chain reaction (PCR) and immune binding properties is required and detection. In each of these tests there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/26C12Q1/68C12Q1/24
Inventor 李振勇杨国翠郭维英陈仕安
Owner 上海浩源生物科技有限公司
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