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Method for synthesizing phytosterol ester in presence of biological enzyme catalyst by using supercritical CO2 as solvent

A technology of phytosterol ester and biological enzyme method, which is applied in the field of catalytic synthesis of phytosterol ester, which can solve the problems of organic solvent residue, high synthesis temperature, and long reaction time

Inactive Publication Date: 2010-09-22
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of long reaction time, high synthesis temperature and organic solvent residue in the transesterification process in the existing conventional synthesis method, the present invention proposes a method using supercritical CO 2 As a dissolving agent, a method for synthesizing phytosterol esters by bioenzymatic transesterification

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0016] Specific implementation mode one: the steps of this implementation mode are as follows:

[0017] Step 1: Pre-reaction treatment: add 22.5-29.5g vegetable oil sample, 7.5-0.5g phytosterol, and 0.3g Novozym 435 lipase as a catalyst in a stainless steel autoclave, and then add stirring magnetic beads;

[0018] Step 2: Leak test: Inject CO into the stainless steel autoclave 2 gas, leak test;

[0019] Step 3: Replacement gas: use CO 2 Replace the air in the stainless steel autoclave for 3 to 5 times, and the pressure is less than 4.5MPa;

[0020] Step 4: Charge CO 2 : Fill CO into the stainless steel autoclave 2 Gas, the pressure is 6-10MPa, so that it is in a supercritical state at the reaction temperature;

[0021] Step 5: Heating: use a heater to heat the stainless steel autoclave, and the heating temperature rises to 55-95°C;

[0022] Step 6: carry out the reaction: start the magnetic stirring under the condition of constant temperature and constant pressure, the s...

specific Embodiment approach 2

[0025] Embodiment 2: This embodiment differs from Embodiment 1 in that the vegetable oil sample added in step 1 is 28.5 g, and the phytosterol is 1.5 g. Other compositions and steps are the same as Embodiment 1.

specific Embodiment approach 3

[0026] Specific embodiment three: the difference between this embodiment and specific embodiment one is that CO is charged in step four 2 The gas pressure is 8-9MPa. Other components and steps are the same as those in Embodiment 1.

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Abstract

The invention discloses a method for synthesizing a phytosterol ester in presence of a biological enzyme catalyst by using supercritical CO2 as a solvent. The invention relates to a reaction method for use in synthesis of the phytosterol ester by the ester exchange of grease and phytosterol. The method comprises the following steps: I, preparing reactants; II, introducing CO2 to detect leakage; III, regulating temperature and stirring speed and performing a reaction; and IV, centrifuging the reaction product to obtain the phytosterol ester. As the solubility the reactants increases in the supercritical CO2 fluid, the solubility of lipase and the solubility of phytosterol in vegetable oil increase respectively. The enzyme can act on glycerol triester sn-3 position fatty acid easily, the fatty acid and diglyceride, which are obtained by enzymolysis, react with the phytosterol respectively to form phytosteryl fatty acid ester and diacylglycerol sterol ester, no solvent is added, and the conversion rate of the obtained sterol ester is improved by over 5 percent compared with the conventional method.

Description

Technical field: [0001] The present invention relates to a kind of supercritical CO 2 It is a method for catalyzing and synthesizing phytosterol esters by a dissolving agent bioenzyme method. Background technique: [0002] Sterols are a class of steroids, mainly including β-sitosterol, stigmasterol, campesterol, brassicasterol, etc. Most of the sterols in vegetable oils are β-sitosterol. As a natural organic compound, phytosterols are mainly purified from the by-products of vegetable oil processing. In recent years, studies have shown that phytosterols are a class of physiologically active substances, widely used in medicine, food, cosmetics and other industries, animal growth agents, plant growth hormones, chemicals, textiles and other fields. [0003] The human body itself cannot synthesize phytosterols and must be ingested from the outside world. Vegetable oils, cereals, fruits and vegetables are rich in phytosterols (the content of phytosterols in vegetable oils is 2...

Claims

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Application Information

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IPC IPC(8): C12P33/00
CPCY02P20/54
Inventor 胡立志王立琦于殿宇王腾宇朱秀清江连洲常云鹤周晓丹
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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