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Method for extracting polyphenol active ingredient in geranium wilfordii through high-efficiency enzyme induction

An active ingredient, the technology of geranium, applied in the field of high-efficiency enzyme-induced extraction of polyphenol active ingredients in geranium, to achieve the effects of increasing content, accelerating freeing, and reducing production costs

Inactive Publication Date: 2010-10-06
NORTHEAST FORESTRY UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

[0007] There are some reports on the extraction of oil and protein from oil crops by enzyme pretreatment and the extraction of active components in plant materials by negative pressure cavitation-assisted extraction technology, but the combination of enzyme induction technology and negative pressure cavitation extraction technology to extract old Active ingredients in stork grass have not been reported yet

Method used

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  • Method for extracting polyphenol active ingredient in geranium wilfordii through high-efficiency enzyme induction
  • Method for extracting polyphenol active ingredient in geranium wilfordii through high-efficiency enzyme induction
  • Method for extracting polyphenol active ingredient in geranium wilfordii through high-efficiency enzyme induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare an enzyme solution with a cellulase concentration of 100 U / mL and a hemicellulase concentration of 50 U / mL, and adjust the pH to 5.3. Weigh 10g of dried geranium, add the enzyme solution at a ratio of 30mL / g of the volume of the enzyme solution to the mass of the raw material, homogenize for 2 minutes, transfer the homogenate into a container and place it in a constant temperature shaker at 40°C for 5 hours of enzymatic hydrolysis. After the hydrolysis process, the enzymolysis solution was transferred into the extraction container, and negative pressure cavitation assisted extraction twice for 15 minutes. After the extract was filtered under reduced pressure, the filtrate was concentrated to dryness to obtain 2.41 g of solids, constant volume with methanol, and using Folin -Phenol reagent method to measure the total polyphenol content, HPLC detection, the content of the total polyphenol active ingredient in the obtained solid is 156.14mgGAE / g, which is 50.9% high...

Embodiment 2

[0027] Prepare an enzyme solution with a hemicellulase concentration of 60 U / mL and a pectinase concentration of 80 U / mL, and adjust the pH to 4.6. Weigh 10g of dried geranium, add the enzyme solution at a ratio of 20mL / g of the volume of the enzyme solution to the mass of the raw material, homogenize for 1min, transfer the homogenate into a container and place it in a constant temperature shaker at 30°C for 5 hours of enzymolysis. After the process, the enzymolysis solution was transferred into the extraction container, and negative pressure cavitation assisted extraction once, each time for 30 minutes, the extract was filtered under reduced pressure and then the filtrate was concentrated to dryness to obtain 2.16 g of solids, constant volume with methanol, and use Folin -Phenol reagent method to measure the total polyphenol content, HPLC detection, the content of the total polyphenol active ingredient in the obtained solid is 134.28mgGAE / g, which is 29.8% higher than the trad...

Embodiment 3

[0029] Prepare an enzyme solution with a cellulase concentration of 70 U / mL and a pectinase concentration of 100 U / mL, and adjust the pH to 5.7. Weigh 10g of dried geranium, add enzyme solution at the ratio of enzyme solution volume to raw material mass ratio of 25mL / g, homogenate for 2min, transfer the homogenate into a container and place it in a constant temperature shaker at 50°C for 6h for enzymatic hydrolysis. After the hydrolysis process, the enzymolysis solution was transferred into the extraction container, and the negative pressure cavitation assisted extraction 3 times, each time for 20 minutes, the extract was filtered under reduced pressure and then the filtrate was concentrated to dryness to obtain 2.25 g of solids, constant volume with methanol, and use Folin-Phenol reagent method measures total polyphenol content, HPLC detects, and the content of total polyphenol active ingredient in the obtained solid is 142.75mgGAE / g, has improved 38.1% compared with tradition...

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Abstract

The invention relates to an extraction technology of active ingredients in geranium wilfordii which is one Chinese medicinal herb. The purpose of the invention is to provide an economic and rapid method for extracting polyphenol active ingredients in geranium wilfordii through high-efficiency enzyme induction. The invention adopts a homogenizer fragmentation high-efficiency enzymatic hydrolysis technology and combines a negative-pressure cavitation assisted extraction technology to obtain geranium wilfordii extract rich in three kinds of polyphenol active ingredients. According to results, the maximum content of total polyphenol active ingredients in the extract can reach 156.14mgGAE / g, the maximum contents of three main polyphenol active ingredients, i.e. gallic acid, Corilagin, Geraniin can respectively reach 9.58mg / g, 6.87mg / g and 19.82mg / g, and the contents are improved by 30-67 percent when compared with a traditional hot reflux method. The invention has the advantages that the high-temperature heating is not required, the organic solvent is not required, the extraction ratio is high, the environment is protected, the operation is safe, the method is suitable for industrialized production, and a novel method and a novel means are provided for the modernization of Chinese medicine extraction.

Description

technical field [0001] The present invention relates to a method for enzymatically inducing to increase the content of active ingredients in Chinese herbal medicines and performing negative-pressure cavitation-assisted extraction on them, that is, to obtain three polyphenol active ingredients rich in gallic acid, corilagin and geranidin High-efficiency enzyme-induced assisted extraction method of geranium extract. Background technique [0002] Geranium [Geranium] is a perennial herb of the genus Geranium in the family Geranaceae. Also known as old view grass, duck foot grass, etc., it is a kind of folk medicine. Geranium has antibacterial, antiviral, and antioxidative effects, and can be used to treat rheumatic arthralgia, numbness and spasm, muscle and bone pain, and diarrhea and dysentery (Zhou Haiyan, 1996; Lei Zhiyong et al., 2002). According to relevant foreign reports, the geranium extract preparation also has the effects of treating enteritis, protecting the liver a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/04A61P31/12A61P39/06A61P35/00A61P9/12A61P1/16A61P7/02
Inventor 付玉杰祖元刚李吉孔羽杨雨春吴楠
Owner NORTHEAST FORESTRY UNIVERSITY
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