Preparation of collagen-chitosan-laser micropore dermal matrix composite membranes
A dermal matrix, chitosan technology, applied in medical science, prosthesis, etc., can solve problems such as fast degradation rate, slow vascularization, and failure to meet clinical application requirements.
Inactive Publication Date: 2011-11-30
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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Problems solved by technology
However, the material is easily degraded in the body, and its mechanical strength is not enough to meet the requirements of clinical application.
The US Patent No. US 5282,859 introduces a double-layer artificial skin. The epidermis is a high-purity microporous collagen gel film, and the dermis is a porous collagen sponge cultured with fibroblasts. Glutaraldehyde crosslinking improves its Tensile strength, but its tensile strength can not meet the requirements of clinical application, and the degradation rate is too fast, and glutaraldehyde is toxic to cells
The Chinese patent No. CN1387,923 discloses a heterogeneous acellular dermal scaffold and its preparation method. The scaffold is obtained by treating suckling pig skin with trypsin and glutaraldehyde. It has high tensile strength and controllable degradation rate , good histocompatibility, but poor permeability, easy to produce fluid and gas accumulation on the wound surface, and slow vascularization
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[0012] Preparation and disinfection of collagen-chitosan-microporous dermal matrix composite membrane 0.5% chitosan (dissolved in 1% acetic acid), 3g / L collagen solution (dissolved in 0.1% acetic acid) in an ice bath in a ratio of 3:7 Mix and pour into the mold under certain conditions, carefully put the dried microporous allogeneic decellularized dermis into the mixture and spread it flat, and pre-cool in the refrigerator at -80°C for 8 hours; transfer the mold to a pre-cooled freeze dryer, freeze Dry for 24 hours; take out the mold, treat with 1mol / L NaOH for 1 hour, rinse with distilled water until neutral, freeze-dry after pre-cooling again, and place at 100°C for 10 -2 24h dry heat crosslinking in mmHg vacuum drying oven, after packaging 60 Co irradiation disinfection standby.
[0013] Umbilical cord mesenchymal stem cells were digested and cleaned with 0.25% trypsin, and resuspended in DMEM / F12 mixed medium to make a cell suspension, and the collagen-chitosan-microporou...
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The invention relates to preparation of collagen-chitosan-laser micropore dermal matrix composite membranes. The invention discloses a new application of collagen-chitosan, i.e. constructing collagen-chitosan-laser micropore dermal matrix composite membranes in vitro. The invention also discloses a preparation method of collagen-chitosan-laser micropore dermal matrix composite membranes, comprising the following steps: blending collagen and a chitosan solution according to a certain proportion, injecting into a mould, placing dry laser micropore dermal matrix into the mixed solution, carryingout cooling, drying and vacuum dry heat crosslinking to form the collagen-chitosan-laser micropore dermal matrix composite membranes, and irradiating with <60> Co and sterilizing for standby application. The invention provides novel biological support materials for skin tissue engineering; the biological support materials possess certain mechanical strength, favorable biocompatibility and no immunogenicity; the raw materials are wide and easy to obtain; and the preparation is easy.
Description
technical field [0001] The invention relates to a new application of the collagen-chitosan-laser microporous dermis matrix composite membrane, in particular to the research as a scaffold material for skin tissue engineering. The invention also relates to a preparation method of the collagen-chitosan-laser microporous dermis matrix composite membrane. Background technique [0002] In terms of bio-scaffold materials, in the current research on tissue-engineered skin, the dermal matrix mostly uses high-molecular protein or polymer materials such as chondroitin sulfate, aminodextran, and polyglycolic acid, which are quickly degraded after being applied to the wound. Absorption, so the tissue engineered skin is only a temporary biological dressing. The acellular dermal matrix is used as a scaffold to culture epidermal cells to construct a skin substitute. Experiments have confirmed that the laser microporous dermal matrix is a good three-dimensional cell culture scaffold, wh...
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IPC IPC(8): A61L27/60
Inventor 柴家科韩焱福孙天骏李东杰陶然朱桂英杨红明宋慧锋梁黎明
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA