Leaf-shaped control gene SRNL1 of rice and application thereof

A gene and rice technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2012-10-03
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are as many as 12 main rice leaf curl genes reported so far, 3 of them have been finely mapped, and only 2 genes related to leaf curl regulation have been cloned.

Method used

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  • Leaf-shaped control gene SRNL1 of rice and application thereof
  • Leaf-shaped control gene SRNL1 of rice and application thereof
  • Leaf-shaped control gene SRNL1 of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Rice material

[0044] The rice (Oryza sativa L.) mutant (Semi-rolled and narrow leaf1) (srnl1) used in the present invention is made from the conventional japonica rice variety "Nihonbare" seed (japonica) After being treated with a chemical mutagen (ethyl methane sulphonate, EMS) at a concentration of 1%, the phenotypically stable semi-rolled narrow-leaf mutant srnl-1 was obtained through mass screening. The original wild-type material is "Nipponbare" japonica rice variety .

[0045] 2. Analyze and target groups

[0046] The homozygous srnl1 mutant was crossed with the common indica variety Taizhong Local No. 1 (TN1) with flat leaves, and the F1 generation was selfed to obtain an F2 population, from which 1451 srnl1 mutant individuals (whose leaves were half-curled and narrow leaves) were selected. as a targeting group. At the early stage of tillering, about 0.5 grams of young leaves were taken from each plant to extract the total DNA.

[0047] 3. SSR and STS ma...

Embodiment 2

[0060] Transformation of plant transformation vector pCAMBIA1300 and plasmid transformation:

[0061] Design 1 pair of primers Vsrnl-1300 (F: GC TTAATTAAGCCGTCGCTG; R: GC ATTTAAATCCGACGAGGG), wherein the front primer contains the BamH I restriction site sequence (GGATCC), and the rear primer contains the Hind III (AAGCTT) and Swa I (ATTTAAA) restriction site sequence; the synthetic Vsrnl-1300 primer PCR amplification Genomic DNA of Nipponbare was amplified to obtain a 421bp fragment (SEQ ID NO: 4) that does not contain all the restriction site sequences on the pCAMBIA1300 multi-cloning site. Dicer performed double digestion and recovered a 411 bp target fragment (SEQ ID NO: 5). The fragment was connected to the pCAMBIA1300 vector after double digestion with BamH I and Hind III endonucleases, so that the Swa I restriction site was introduced between the multiple cloning sites BamH I and Hind III of the pCAMBIA1300 vector . Then the BAC clone OSJNBa0079G12 was completely d...

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Abstract

The invention discloses protein encoded by a leaf-shaped control gene SRNL1 of rice. The protein has an amino acid sequence shown as SEQ ID No.2. The invention also discloses a gene for encoding the protein. The gene has a nucleotide sequence shown as SEQ ID No.1. The gene can be used for culturing the rice with curled leaves.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a method of cloning rice SRNL1 (Semi-Rolled and Narrow Leaf 1) gene by map-based cloning technology, identifying the function of the gene by means of transgenic technology by constructing a complementary vector; Changes in the number of tube bundles and morphological changes in vesicular cells can adjust the width and curl of rice leaves to obtain the ideal plant shape of the crops, improve the photosynthetic efficiency of the leaves, and increase the yield of the crops. Background technique [0002] As a model plant of monocots, rice has the typical characteristics of monocot leaves. Rice leaves are composed of blades and leaf sheaths, and blades can be divided into three parts: epidermis, mesophyll layer and vascular layer. As an important aspect of plant morphogenesis, leaf development is a very complex process, including cell division a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N1/21C12N5/10A01H4/00A01H5/00
Inventor 钱前张光恒曾大力胡江董国军颜美仙朱丽高振宇郭龙彪
Owner CHINA NAT RICE RES INST
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