Application of monascus color components and derivatives thereof in fighting cancers
A technology of monascus pigments and derivatives, which is applied in the fields of drug biotechnology and drug development, can solve the problems that have not been further disclosed, and achieve the effects of less toxic side effects and high-efficiency inducing apoptosis activity.
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Embodiment 1
[0034] Preparation of Monascus Pigment Crude Extract:
[0035] Steam the rice until it is fully cooked, inoculate Monascus after cooling, and place it for solid-state fermentation at 30°C for 8 days, and the color value of the fermented product is 5100 U / g (E 505nm), containing red pigment (erythromycin and monascus amine) 10.1 g / Kg, orange pigment (erythematin and monascus red pigment) 48.3 g / Kg, yellow pigment (monascus and monascus xanthin) 41.9 g / Kg.
[0036] The fermented product is extracted with 70% (weight ratio, the same below) ethanol solution (water bath at 60°C, solid-to-liquid ratio 1:10), then concentrated in vacuum to 1 / 2 of the original volume, and then the crude extract of the red yeast pigment component is precipitated. The yield 10.1%, the pigment component accounts for 78.9% of the total weight of the crude extract.
[0037] Human liver cancer cells Hep-G2, human neuroma cells SH-5Y, colon cancer cells HT-29, human gastric cancer cells BGC-823, AGS and ...
Embodiment 2
[0039] Preparation of Monascus Pigment Purified Components:
[0040] Preparation of Monascus Pigment Purification Components: The crude pigment extract prepared in Example 1 was separated and purified by HPLC (C18 column), the elution condition was 80% methanol solution, and Monascus, Monascus Red and Erythematamine were collected respectively Component elution samples were vacuum concentrated to 1 / 2 of the original volume and then crystals were precipitated. Monascus, monascus rubin and erythematamine component crystals were determined by HPLC-MS, and their molecular weights were 358, 382 and 353, respectively, and the purity 99.3%, 98.6% and 98.9%.
[0041] Human liver cancer cells Hep-G2, human neuroma cells SH-5Y, colon cancer cells HT-29, human gastric cancer cells BGC-823, AGS and MKN45 were routinely cultured at 37°C and 5% CO2. Cancer cells were inoculated in a 96-well plate with a cell concentration of 2500 cells / well. The components of monascus, monascus and erythem...
Embodiment 3
[0043]Preparation of monascus rubin derivative N-glutaryl monascus red amine: Take 1 g of monascus rubin prepared in Example 2 and dissolve it in 100 mL of 70% ethanol solution, add 10 mL of glutamine at 60 °C Sodium monosodium acid (1mol / L), purified by silica gel column after reaction for 1 hour, eluted with anhydrous methanol, collected the eluted sample of N-glutaryl monascus red amine component, concentrated in vacuum to 1 / 2 of the original volume and then precipitated Crystal, N-glutaryl monascus red amine crystal was determined by HPLC-MS, its molecular weight was 511, and its purity was 99.1%.
[0044] Preparation of erythematamine derivative 4-hydroxyerythematamine: 1 g of erythematamine prepared in Example 2 was dissolved in 100 mL of 70% ethanol solution, and 5 mL of sodium borohydride (1mol / L) was added at 30°C to react After 1 hour, it was purified by silica gel column, eluted with anhydrous methanol, and the eluted sample of 4-hydroxyerythematamine component was ...
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