Extracting method of gamma-polyglutamic acid

A polyglutamic acid and extraction method technology, which is applied in the field of chemical polymer extraction, can solve the problems of complex extraction process, high production cost, and difficulty in realizing industrialized production, and achieve the effects of high yield, low cost, and novel process route

Inactive Publication Date: 2010-11-10
郸城财鑫糖业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, γ-PGA is mainly obtained through liquid fermentation. The extraction processes mainly include: organic solvent precipitation method, chemical precipitation method or membrane separation precipitation method, etc. Due to the disadvantages of high production cost and complicated extraction process in the existing extraction process, it is difficult to realize industrialization Production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The extraction method of a kind of gamma-polyglutamic acid of the present invention is specific as follows:

[0024] (1) Detection of raw materials

[0025] The content of polyglutamic acid in the fermentation broth was detected to be 52g / L, the pH was 6.3, the content of soluble protein was 0.46g / L, and the volume was 100L.

[0026] (2) Sterilization by filtration

[0027] Start stirring, adjust the fermented liquid to pH 3.0 with 20% hydrochloric acid, and then use a high-speed centrifuge at 10,000-15,000 rpm to separate and sterilize bacteria, and pump the separated supernatant to an enzymatic hydrolysis tank.

[0028] (3) Enzyme removes protein

[0029] In the enzymolysis tank, use 20% lye to adjust the fermentation broth to 6.0, heat to 40°C, add 1‰ sodium hexametaphosphate and 0.25‰ lysozyme for 1 hour, then adjust the pH to 7.5, and heat to 55 Add 1‰ of alkaline protease at ℃ for 30 minutes, then heat to 85℃ and keep it for 20 minutes to inactivate the enzyme....

Embodiment 2

[0037] The extraction method of a kind of gamma-polyglutamic acid of the present invention is specific as follows:

[0038] (1) Detection of raw materials

[0039] The content of polyglutamic acid in the fermentation broth was detected to be 52g / L, the pH was 6.3, the content of soluble protein was 0.46g / L, and the volume was 100L.

[0040] (2) Sterilization by filtration

[0041] Start stirring, adjust the fermented liquid to pH 5.0 with 20% hydrochloric acid, then use a high-speed centrifuge at 10,000-15,000 rpm to separate and sterilize bacteria, and pump the separated supernatant to an enzymolysis tank.

[0042] (3) Enzyme removes protein

[0043] In the enzymolysis tank, use 20% lye to adjust the fermentation broth to 7.0, heat to 50°C, add 1‰ sodium hexametaphosphate and 0.25‰ lysozyme for 1 hour, then adjust the pH to 8.0, and heat to 60 Add 1‰ of alkaline protease at ℃ for 30 minutes, then heat to 90℃ and keep it for 20 minutes to inactivate the enzyme.

[0044] (4...

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Abstract

The invention discloses an extracting method of gamma-polyglutamic acid (gamma-PGA), in particular to an extracting method, which comprises the following steps: adjusting the pH value to be 3.0-5.0 with dilute acid; filtering and degermming; adding protease and lysozyme to remove protein; adding 2-3 times of volume of ethanol and precipitating; filtering and dissolving with distillated water; filtering by using an ultrafiltration membrane to remove impurities and condensing; and spraying and drying to obtain a finished product of the gamma-polyglutamic acid. The invention has the advantages of novel and simple process route, low production cost, high yield and high suitability for industrial production.

Description

technical field [0001] The invention relates to a method for extracting extracellular polymers, belonging to the field of chemical polymer extraction, in particular to a method for extracting gamma-polyglutamic acid (PGA). Background technique [0002] γ-PGA is an extracellular polymer produced by a variety of bacilli, and is the main component of some microbial capsules. A variety of γ-PGA-producing strains have been found, and the main production strains are concentrated in subtilis Bacillus and Bacillus licheniformis. [0003] The γ-PGA molecular chain has a large number of side chain carboxyl groups, which can form hydrogen bonds within or between molecules, so it has extremely high water absorption and moisturizing properties. γ-PGA is easy to combine with some drugs to form a stable complex, which is an ideal biodegradable medical polymer material. γ-PGA is non-polluting to the environment and has many unique physical, chemical and biological properties such as excel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
Inventor 仵树仁李霄峰王晓飞李锦王念详陆启明闫锁朱瑞清袁淏李龙伟展现明张兵团
Owner 郸城财鑫糖业有限责任公司
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