Corynebacterium humireducens and application thereof

A corynebacterium and humus technology, applied in the direction of bacteria, biochemical fuel cells, microorganisms, etc., can solve the problems of unfavorable microbial growth, and no strains with humus reduction and electricity production activity have been found, and achieve high efficiency humus reduction ability and electricity production activity, Stable electricity production and broad application prospects

Inactive Publication Date: 2010-11-24
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alkaline conditions help to improve the efficiency of electricity production, but the alkaline environment is not conducive to the growth of most microorganisms, so this patent aims to develop alkaline

Method used

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  • Corynebacterium humireducens and application thereof
  • Corynebacterium humireducens and application thereof
  • Corynebacterium humireducens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and purification of humus-reducing Corynebacterium of the present invention

[0035] 1) Take 5mL anolyte from the sludge microbial fuel cell with pH 10 and inoculate it in 100mL liquid separation medium.

[0036] The structure of the sludge microbial fuel cell has been described in detail in the patent application No. 200910041235.4.

[0037] The composition of described liquid separation medium is: contain 1.0g glucose (electron donor), 16g AQDS (electron acceptor), 2.5g NaHCO in every liter of liquid separation medium 3 , 0.25gNH 4Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution, the pH value is 10. Among them, the vitamin solution contains 2.0 mg biotin, 2.0 mg folic acid, and 10.0 mg vitamin B per liter of deionized water. 6 , 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin B 12 , 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; the trace element solut...

Embodiment 2

[0042] Embodiment 2: Physiological and biochemical characteristics of bacterial strain (Corynebacterium humireducens)

[0043] The strain is a non-fermentative facultative anaerobic bacteria, resistant to salt and alkali. The growth temperature ranges from 25 to 40°C, and cannot grow above 45°C and below 10°C; the growth pH range is 7.0 to 11.0 (optimum: pH9.0); the NaCl% required for growth is 0 to 12% (> 13% cannot growth). According to the physiological and biochemical characteristics, the bacterium was identified as Corynebacteria.

[0044] Table 1 Physiological and biochemical characteristics of the inventive strain

[0045]

[0046]

[0047] + indicates a positive reaction; - indicates a negative reaction

Embodiment 3

[0048] Example 3: Molecular taxonomic status of strains (Corynebacterium humireducens)

[0049] as attached figure 2 As shown, the 6 strains with the closest homology of the invented strain all belong to Corynebacterium, which are respectively: C.marinum (similarity 96.9%, 1393bp), C.testudinoris (96.8%, 1395bp), C.halotolerans (96.4% , 1392bp), C.felinum (96.0%, 1383bp), C.singulare (96.0%, 1297bp) and C.freiburgense (95.9%, 1395bp). According to the phylogenetic theory, only when the similarity between the strains is greater than 97%, can they be considered as the same strain, and the similarity between the target strain and its closest strain C.marinum is only 96.9%, so the strain is defined as a new species.

[0050] Combining the above physiological and biochemical characteristics and the results of 16SrRNA sequence comparison, the Corynebacterium humireducens of the present invention belongs to the genus Corynebacterium, is a new species of this genus, and is named Cor...

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Abstract

The invention discloses corynebacterium humireducens and an application thereof. The corynebacterium humireducens is obtained by separating and purifying anolyte of a sludge microbe fuel cell, and is preserved in the ordinary microbe centre of the China Microbe Culture Preservation Management Committee on April 14, 2008 with the preservation number of CGMCC No.2452. The corynebacterium humireducens has wide utilization spectrum of electron donor under the anaerobic condition, has efficient humus reduction capability and electrogenesis capability under the alkaline condition, can be widely applied to in-situ microbe restoration of organic pollutants, microbe fuel cell generating technology or recycling processing of high-concentration organic wastes.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and specifically relates to a humus-reducing Corynebacterium strain with electricity production and humus respiration activity and its application. technical background [0002] Humus respiration is a new type of microbial energy metabolism that has attracted much attention in recent years. Humus respiration, also known as quinone respiration, refers to the fact that microorganisms use humus as the terminal electron acceptor, couple the reduction of humus through the oxidation of electron donors, and store the energy of life activities from this process. The microorganisms capable of humus respiration are called humus reducing bacteria. At present, the environmental importance of humic respiration has been widely accepted and recognized: humic respiration is not only coupled with the natural cycle process of carbon, nitrogen, phosphorus and other elements, but also significant...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/15
CPCY02E60/527Y02E60/50
Inventor 周顺桂武春媛王跃强李芳柏袁勇
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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