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Method for producing Calvatia gigantea by utilizing spent wash

A technology for producing big bald puffballs and bottom pot water, applied in the direction of botany equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of waste of resources, environment, pollution, etc., and achieve the effect of saving production costs

Inactive Publication Date: 2012-07-04
YIBIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional process directly discharges the bottom pot water, causing waste of resources and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The method of producing big bald puffball mycelium by using bottom pot water, take 1.2 grams of potato flour, 3.5 grams of corn flour, 0.6 grams of ammonium sulfate, 1.2 grams of yeast powder, 0.05 grams of copper sulfate and 0.09 grams of calcium carbonate and mix evenly, add to the bottom 100 ml of pot water was completely dissolved, the solution was acidic, and then sodium hydroxide was added to adjust the pH to 6.0 to obtain the culture medium.

[0017] Take 100 ml of the prepared culture medium and put it into a 500 ml Erlenmeyer flask, sterilize it at 121°C for 25 minutes, then cool it to room temperature, insert 12 ml of Puffball baldum strain, keep the temperature at 23°C, 210 rpm Cultivate for 4.5 days, filter, and dry at 60° C. under the condition of continuous stirring at a rotating speed of 3.0 grams per minute to obtain 3.0 grams of mycelia of the bald puffball with a dry weight.

Embodiment 2

[0019] The method of using bottom pot water to produce big bald puffball mycelium, take 0.8 grams of potato flour, 3.0 grams of corn flour, 0.5 grams of ammonium sulfate, 1.0 grams of yeast powder, 0.03 grams of copper sulfate and 0.08 grams of calcium carbonate and mix them evenly, and then mix them with the bottom pot 100 ml of pot water was completely dissolved, and then the pH was adjusted to 5.7 to obtain the culture medium.

[0020] Take 100 ml of the prepared medium and put it into a 500 ml Erlenmeyer flask, sterilize at 121°C for 25 minutes, then cool to room temperature, insert 10 ml of Puffball baldum strain, keep the temperature at 23°C, 180 rpm Under the condition of continuous stirring at a speed of 1 / min, culture for 4 days, filter, and dry at 60° C., can obtain 2.8 grams of dry weight mycelium of the puffball puffball.

Embodiment 3

[0022] Utilize bottom pot water to produce the method of big bald puffball mycelium, get potato flour 1.0 grams, corn flour 2.5 grams, ammonium sulfate 0.2 grams, yeast powder 0.5 grams, copper sulfate 0.07 grams and calcium carbonate 0.02 grams mix evenly, then mix with 100 milliliters of bottom pot water is completely dissolved, and then its pH is adjusted to 5.7 to obtain the fermentation medium.

[0023] Take 100 ml of the prepared culture medium and put it into a 500 ml Erlenmeyer flask, sterilize it at 121°C for 25 minutes, then cool it to room temperature, add 9 ml of Puffball baldum strain, keep the temperature at 23°C, 170 rpm Under the condition of continuous stirring at a speed of 1 / min, culture for 6 days, filter, and dry at 60° C., can obtain 2.5 grams of dry weight mycelia of the bald puffball.

[0024] The bottom pot water used in the above examples has been defined and quantitatively analyzed before. It is worth noting that: in actual production, the content of...

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PUM

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Abstract

The invention discloses a method for producing Calvatia gigantea by utilizing strong aromatic Chinese spirit byproduct spent wash, which belongs to the technical field of fermentation engineering. In the culture medium taking spent wash as a main raw material, the Calvatia gigantea is cultured by controlling certain fermentation conditions and time, and the dry weight of Calvatia gigantea produced by every 100 ml of fermentation liquid can reach 2.5-3.1 g. The invention cultures edible and medicinal Calvatia gigantea by using strong aromatic Chinese spirit byproduct spent wash as the main rawmaterial and using potato flour, corn flour, ammonium sulfate, yeast powder, cupric sulfate and calcium carbonate as the auxiliary materials; and thus, the invention greatly lowers the production cost, shortens the production time and reduces the environmental pollution of spent wash.

Description

technical field [0001] The invention relates to a method for producing mycelia of big bald puffballs, in particular to a method for producing big bald puffball mycelium by using bottom pot water as a by-product of Luzhou-flavor liquor production as a main raw material. Background technique [0002] The big bald puffball, also known as the big puffball, belongs to the subclass Agaricus, the order Agaricaceae, and the family Puffaceae. Big bald puffballs are rich in amino acids and a variety of active substances that have anti-inflammatory, anti-bacterial, anti-cancer, anti-aging, anti-ulcer, immune-enhancing and radiation-proof effects. In recent years, the clinical application of big bald pistachio in internal medicine, surgery, ENT and other diseases has achieved good curative effect and has a good development prospect. At the same time, the bald puffball is also a common medicinal fungus used for detumescence, detoxification and hemostasis. Its preparations have been test...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 朱文优王涛蒲岚张超钟桃田源
Owner YIBIN UNIV