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Yeast and application thereof in decolorization

A technology of Candida tropicalis and strains, applied in the direction of fungi, microbial-based methods, textile industry wastewater treatment, etc., can solve the problems of lack of degrading strains, poor broad-spectrum, salt resistance or alkali resistance, etc. Achieve the effect of broad-spectrum decolorization and degradation ability, simple cultivation method and strong decolorization ability

Inactive Publication Date: 2010-12-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a big textile and dyeing country in our country, the problem of dye wastewater treatment has not been well resolved.
[0006] 1. Most of the decolorization is achieved through the adsorption of the bacteria to the dye, and the dye cannot be further degraded and completely mineralized
[0007] 2. Lack of efficient degradation strains
Most single colonies have poor broad-spectrum and can only act on a few dye molecules with simple structures; and the decolorization rate cannot meet the treatment requirements of actual wastewater
[0008] 3. Poor adaptability to the environment
The salt tolerance or alkali resistance of most strains is not strong, and the growth ability and decolorization and degradation ability of the bacteria decrease rapidly under high salt concentration

Method used

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  • Yeast and application thereof in decolorization
  • Yeast and application thereof in decolorization
  • Yeast and application thereof in decolorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Isolation, purification and identification of Candida tropicalis YL3-1CGMCC No.34322.

[0029] The screening process of Candida tropicalis provided by the invention is to obtain the activated sludge sample from the printing and dyeing wastewater treatment plant, according to figure 1 Described flow process is screened, and its specific screening steps are as follows:

[0030] Extract sludge from a wastewater pool of a printing and dyeing factory in Zhejiang, weigh 1g and break it up with glass beads, then mix well with 100ml of sterile water, take 3-4ml of the liquid and put it into the sterilized enrichment medium, at 30°C, 170 revolutions for 12 hours, and the enriched samples were made into 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 A total of 7 dilutions, each dilution draws 0.2ml and spreads it on the dye medium plate, and cultures it statically at 30°C for 24 hours, picks the colony with a decolorized circle and purifies it three times on th...

Embodiment 2

[0036] Example 2, verification of the decolorization effect of Candida tropicalis YL3-1CGMCC No.3432 on acid scarlet GR

[0037] Candida tropicalis (Candida tropicalis) YL3-1CGMCC No.3432 was inoculated into a Erlenmeyer flask equipped with YPD medium, and the temperature was set at 30°C, and the rotation speed was 170rpm on a shaker for 12-16h, according to 5% (v The inoculum amount of / v) was transferred to the GR dye culture medium of different acid scarlet concentrations respectively (concrete concentrations such as figure 2 Shown), cultured in an aerobic mode (the aerobic mode is shaking culture in a shaker flask) for 12 hours in a shaker with a temperature set at 30°C and a rotation speed of 170 rpm.

[0038] After the cultivation, take a certain amount of culture solution into the centrifuge tube, centrifuge at 13000rpm for 10min, take the supernatant and measure the OD value at the maximum absorption wavelength of the dye at 510nm on a spectrophotometer, and use the d...

Embodiment 3

[0047] Example 3, Candida tropicalis (Candida tropicalis) YL3-1 CGMCC No.3432 decolorizes direct suntan SA and basic orange

[0048] Candida tropicalis (Candida tropicalis) YL3-1CGMCC No.3432 was inoculated into a Erlenmeyer flask equipped with YPD medium, and the temperature was set at 30°C, and the rotation speed was 170rpm on a shaker for 12-16h, according to 5% (v The inoculation amount of / v) was respectively inoculated into the direct sunfast black SA dye medium and the basic orange dye medium, and cultivated in an aerobic manner at 30°C and 170rpm for 24h, and the culture solution was centrifuged according to the method described in Example 2. Methods The OD value was measured at the maximum absorption wavelength of the dye and the corresponding decolorization rate was calculated.

[0049] The experimental results showed that the decolorization rate of the strain to 50mg / L direct sun black SA was 97%, and the decolorization rate to 50mg / L basic orange was 96%.

[0050]...

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Abstract

The invention discloses yeast and application thereof in decolorization. The strain is Candida tropicalis YL3-1 CGMCC No. 3432 which has broad-spectrum azo dye decolorization and degradation capacity, can act on various azo dyes with different structures and has high decolorization capacity. The strain has the advantages of effectively decoloring high-salinity azo dye wastewater, along with simple culture method, high growth speed and high environmental applicability. The strain is used for the decolorization of printing wastewater and industrial wastewater associated with the use of the dye, can solve the problems existing in the prior art and has industrial application prospect.

Description

technical field [0001] The invention relates to a strain of yeast and its application in decolorization, in particular to a strain of Candida tropicalis and its application in removing azo dyes in production, sewage and other systems. Background technique [0002] With the continuous discharge of industrial wastewater such as dye synthesis, printing and dyeing, the number and types of dyes entering the environment continue to increase, resulting in increasingly serious environmental pollution. Among them, azo dyes account for about 70% of the world's total annual dye production, and are the largest category of industrial dyes. Dye wastewater has the characteristics of large chroma, high concentration of organic matter, complex components, many refractory substances, and a large amount of inorganic salts. It is a difficult-to-treat industrial wastewater. As a big textile and dyeing country in our country, the problem of dye wastewater treatment has not been well resolved. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/02C02F3/34C12R1/74C02F103/30C02F103/36
CPCY02W10/10
Inventor 陈必强张胜琴柳皋隽杨光曹竹安
Owner TSINGHUA UNIV
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