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Application of ethylmethylsulfone in preparation of chemical anthericide for crops, and breeding

A technology of ethyl methanesulfonate and andricide, which is applied in the field of preparation of chemical andricide for Chinese cabbage and breeding, can solve the problems of long operation time, large field operation area, environmental pollution, etc., and achieve shortening The effect of processing time, improving the killing rate, and reducing the workload

Inactive Publication Date: 2010-12-15
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the large-scale production of first-generation hybrids using the above-mentioned chemical detasseling method has been widely used in rapeseed and other crops, there are many defects in this application method, such as long operation time, which needs to run through the entire flowering period, large field operation area, and spraying. Incomplete application will lead to low male killing rate, and there will be more or less environmental pollution at the same time

Method used

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  • Application of ethylmethylsulfone in preparation of chemical anthericide for crops, and breeding
  • Application of ethylmethylsulfone in preparation of chemical anthericide for crops, and breeding
  • Application of ethylmethylsulfone in preparation of chemical anthericide for crops, and breeding

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Effect test

Embodiment 1

[0037] A chemical andricide for crops, the components are as follows, all in percent by volume:

[0038] Ethyl methanesulfonate stock solution: 0.8%, sodium phosphate buffer: 99.2%, pH 5.8;

[0039] The concentration of ethyl methanesulfonate in the described ethyl methanesulfonate stock solution is 1.206g / ml, which can be prepared by ethyl methanesulfonate and water in proportion;

[0040] The sodium phosphate buffer consists of 1M Na 2 HPO 4 Solution 7.9ml with concentration of 1M NaH 2 PO 4 The solution is prepared by adding water to 92.1ml to 1000ml.

[0041] Mix the above-mentioned ethyl methanesulfonate stock solution and sodium phosphate buffer evenly.

Embodiment 2

[0043] The experimental site of this embodiment is the experimental site of Vegetable Research Institute of Shandong Academy of Agricultural Sciences (address: south of Sangyuan Road, Jinan City).

[0044] A method utilizing the crop chemical andricide breeding in embodiment 1, the steps are as follows:

[0045] (1) On December 20, the seeds of Chinese cabbage inbred line Z55 were pre-imbibed in 22-28°C water for 4-6 hours to obtain pretreated seeds;

[0046] (2) the pretreatment seed that step (1) is made is soaked in the chemical andricide that embodiment 1 makes, puts into shaker then, shakes for 15 hours, bed speed is 100rpm, then rinses with deionized water for 4 Time, every 15 minutes, must kill male seed; Simultaneously, by ethyl methanesulfonate stock solution and sodium phosphate buffer solution preparation ethyl methanesulfonate stock solution volume percentage content is 0.2% solution and ethyl methanesulfonate Ester stock solution volume percentage composition is ...

Embodiment 3

[0052] Select 3 plants in the gauze shed in step (4) of Example 2 after treatment with the andricide prepared from 0.8% ethyl methanesulfonate stock solution, select the expanded flowers with aborted anthers, and use other inbred lines Z61- 2 pollen for its artificial pollination. After pollination, the fruit pods gradually expanded, and finally a normal F1 generation hybrid was obtained, with a seed setting rate of 100%. This shows that the anther-aborted plants produced by the above-mentioned chemical andricidal technique can accept other Chinese cabbage pollen and produce plump hybrid seeds. Therefore, the chemical andricide can be used to significantly improve the purity of hybrid seed production (≥99%), and finally achieve large-scale production The purpose of high-purity Chinese cabbage first-generation hybrids.

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Abstract

The invention relates to application of ethylmethylsulfone in preparation of a chemical anthericide for crops, and breeding, in particular to application of the ethylmethylsulfone in preparation of a chemical anthericide for cabbages, and breeding, which belongs to the technical field of plant breeding. The chemical anthericide for the crops comprises the following components in percentage by volume: 0.7 to 0.9 percent of stock solution of ethylmethylsulfone and the balance of buffer solution of sodium phosphate, wherein the pH value is 5.6 to 6.0. The stock solution of ethylmethylsulfone with the concentration of 0.7 to 0.9 percent is used for performing male-killing, so the invention provides a novel anthericide for the field of breeding. By using the anthericide, the processing time is greatly shortened, the work load of breeding is reduced, and the male-killing rate is greatly increased.

Description

technical field [0001] The invention relates to the application of ethyl methylsulfonate in the preparation of chemical andricides for crops and breeding, in particular to the application of ethyl methylsulfonate in the preparation of chemical andricides for Chinese cabbage and in breeding, and belongs to the technical field of plant breeding . Background technique [0002] Ethyl methanesulfonate (EMS) is one of the important carcinogens. In biology, it is often used to create mutant libraries, broaden parental resources, and innovate breeding methods. However, the use of EMS must be protected to prevent the induction of cancer. The mutation induced by EMS is mainly completed through two steps. First, the 06 position of guanine is alkylated to become a positively charged quaternary ammonium group, so that two genetic effects occur: one is the pairing of alkylated guanine with thymine , instead of cytosine, a conversion mutation occurs; the second is due to the activation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01H1/02A01N41/08A01P21/00
Inventor 高建伟李化银李利斌刘立锋王凤德张庶
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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