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Molecular fluorescent switch for detecting intracellular zinc ions and application thereof
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A fluorescent switch, zinc ion technology, applied in fluorescence/phosphorescence, biological testing, luminescent materials, etc., can solve the problems of unreported application of fluorescent probe molecules, complex preparation of zinc ion fluorescent probes, etc., to achieve simple structure and identification. Good, easy-to-synthesize effects
Inactive Publication Date: 2013-04-24
ZHENGZHOU UNIV
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Moreover, the application of corresponding fluorescent probe molecules in biological detection has not been reported.
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Embodiment 1
[0020] Embodiment 1 Preparation of fluorescent probe of the present invention
[0021] In a 100ml single-necked round-bottomed flask equipped with a spherical condenser, add an equivalent amount of p-hydroxyaniline and 4-methyl-7-acetamido-1,8-naphthyridinaldehyde to fully dissolve them in methanol. Sealed system, argon as protective gas, degassing. Reflux 5h, the reaction is over. After rotary evaporation, the crude product was separated and purified by silica gelchromatography (200-300 mesh) with dichloromethane as the eluent to obtain the product as a yellow solid. It was then recrystallized from methanol.
[0025] Embodiment 2 Application of the fluorescent probe of the present invention
[0026] Prepared 5.0×10 -5 M fluorescent probe ethanol solution, and various metal ions 1.0×10 -2For the ethanol solution of M, get two milliliters of the probe solution prepared above in a cuvette, gradually add the zinc ion solution in the cuvette, and detect the impact of the addition of the zinc ion on the fluorescence spectrum properties of the probe with a fluorescence spectrometer. The influence test of other metal ions on the fluorescence spectrum of the probe is similar to that of zinc ions. The interference research of other metal ions on the zinc ion fluorescent probe is similar to that of zinc ion.
[0027] Combined with the accompanying drawings, spectral analysis shows that the fluorescent probe molecule of the present invention has high selectivity for zinc ions, and the fluorescence of the low-concentration solution of the fluorescent probe molecule is significantly enhanced af...
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Abstract
The invention discloses a molecular fluorescent switch for detecting intracellularzinc ions and application thereof and belongs to the technical field of biological analysis and detection. A fluorescent probe molecule has the following structural general formula that: in the general formula, R1 represents alkyl, and R2 represents alkyl and hydroxyl. A fluorescent molecular probe has the characteristics of relatively simple structure, easy synthesis and the like. The molecular fluorescent switch can be applied to metalion detection, particularly detection of zinc ions in biological tissues. The molecular fluorescent switch has the advantages of good recognition effect in cells, capability of realizing real-time fluorescence imaging of biological cells or tissues and realizing trace zincionfluorescence detection under the condition of interfering ions and good application and development prospects.
Description
technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular relates to the highly selective detection of zinc ions, in particular to a molecular fluorescence switch which can be used for the determination of zinc ions in living cells. Background technique [0002] Compared with other detection methods, the molecular fluorescent switch has the advantages of detecting metal ions: it can achieve high detection sensitivity for a single ion; the outermost layer of zinc ions is 3d 10 4s 0 Electron configuration, usually using many conventional analytical methods such as UV-Vis spectroscopy, Spectroscopy, nuclear magnetic resonancespectroscopy (NMR), paramagnetic resonancespectroscopy (EPR), etc. cannot obtain suitable spectral signals or magnetic signals. However, the use of fluorescent molecular probes to detect zinc ions and copper ions has the characteristics of fast response, high sensitivity, low detection thres...
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