Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof

Abstract

The invention relates to a ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and a preparation method thereof. The problem of detecting the beta-stimulants the albuterol, the clenobuterol hydrochloride and the ractopamine simultaneously is solved. A parent material PVC plate is sequentially provided with a diversion glass fiber, a carrier glass fiber, a nitrocellulose membrane and a suction cotton pulp plate from front to back; the diversion glass fiber and the suction cotton pulp plate are covered with membranes; the carrier glass fiber absorbs a monoclonal antibody colloidal gold marker resistant to albuterol protein conjugates, clenobuterol hydrochloride protein conjugates and ractopamine protein conjugates; and the intermediate nitrocellulose membrane is provided with a rabbit anti-mouse polyclonal antibody quality control line and three detection lines which are respectively coated with the albuterol protein conjugates, the clenobuterol hydrochloride protein conjugates and the ractopamine protein conjugates. By the invention, the beta-stimulants the albuterol, the clenobuterol hydrochloride and the ractopamine can be effectively detected simultaneously with convenience, quickness and accurate result.

Description

1. Technical field [0001] The invention relates to the technical field of beta-stimulant detection, in particular to a triple detection test strip for beta-stimulant albuterol, clenbuterol hydrochloride and ractopamine and a preparation method thereof. 2. Background technology [0002] β-agonists are a class of adrenaline mimics, named for their ability to excite (excitate) the β receptors in animal and human tissues to regulate the functions of different tissues in the body. Their chemical components are phenylethanolamine derivatives. The drug has the effect of promoting the decomposition of livestock fat and increasing muscle growth. It is used as a nutrient redistribution agent to increase lean meat percentage, reduce fat deposition and promote animal growth. It is illegally used by some livestock farmers as a breeding accelerator. Clenbuterol, Salbutamol and ractopamine are the most commonly used in the breeding industry. The use of β-stimulants in livestock feeds has c...

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a β-stimulant salbutamol, clenbuterol hydrochloride, ractopamine triple detection test strip and its preparation method, which can effectively solve the problem of rapid and simple The problem of simultaneously detecting the β-stimulant albuterol, clenbuterol hydrochloride, and ractopamine is that the technical solution is that the test strip includes a base material PVC board and a film, and the base material PVC board is arranged in sequence from front to back There are water diversion glass fiber, carrier glass fiber, nitrocellulose membrane and water absorbent cotton pulp board. The water diversion glass fiber and water absorbent cotton pulp board are covered with a film consisting of a front covering film and a rear covering film. The carrier glass fiber is adsorbed with Anti-salbutamol protein conjugates, anti-clenbuterol hydrochloride protein conjugates, anti-ractopamine protein conjugates monoclonal antibody colloidal gold markers; a strip coated with rabbit anti- Mouse polyclonal antibody (for known commercially available products, such as the product of Zhengzhou Lansen Biotechnology Co., Ltd.) quality control line (the said front and back refer to the water-absorbing direction from the direction of water-absorbing, the water-absorbing glass fiber is the front, and the water-absorbing Cotton pulp board is the back), three detection lines coated with albuterol protein conjugates, clenbuterol hydrochloride protein conjugates, and ractopamine protein conjugates respectively. It is in close contact with the front end of the water-absorbent cotton pulp board, and the front end of the carrier glass fiber is in close contact with the rear end of the water-absorbing glass fiber;

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