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Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof

A ractopamine and anti-ractopamine technology, applied in biological tests, measuring devices, material inspection products, etc., can solve problems such as missed detection, unawareness of the use of beta stimulants by farmers, and blindness in detection.

Inactive Publication Date: 2013-04-03
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The regulatory authorities do not know what kind of β-stimulants are used by the farmers, and there is a certain degree of blindness in the detection, which often results in missed detection

Method used

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  • Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof

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Comparison scheme
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preparation example Construction

[0010] The preparation method of the binary detection test strip of the present invention is realized by the following steps: firstly prepare colloidal gold labeled monoclonal antibody, colloidal gold solution, colloidal gold marker, solidified gold standard, coating film respectively, and then prepare the above-mentioned Coating film, solidified gold standard, water diversion glass fiber, and water-absorbing cotton pulp board are fixedly bonded to the base material PVC board, covered with the film, and cut into long strips; specifically:

[0011] 1. Preparation of colloidal gold-labeled monoclonal antibody: using octanoic acid-ammonium sulfate method, take 1 part of mouse ascites containing anti-ractopamine protein conjugate or anti-albuterol protein conjugate monoclonal antibody and 2 parts of 60mM acetate buffer Mix, add octanoic acid 33μl / ml mouse ascites dropwise under stirring at 18-25°C (that is, add 33μl octanoic acid to every 1ml of mouse ascites), mix well, place at r...

Embodiment 1

[0017] Embodiment 1: the present invention can also be to comprise base material PVC board 1, the nitrocellulose membrane 2 that is adhered to the middle part of base material, the water diversion glass fiber 3 and the carrier that are adhered to base material PVC plate 1 front part in concrete implementation Glass fiber 4, water-absorbent cotton pulp board 5 adhered to the back of the base material, upper coating 6 and lower coating 7, the rear end surface of the water diversion glass fiber 3 and the front end surface of the carrier glass fiber 4 are closely connected, and the nitrocellulose membrane 2 two The end faces are in close contact with the rear end face of the carrier glass fiber 4 and the front face of the absorbent cotton pulp board 5 respectively, and the lower carrier glass fiber 4 is anti-ractopamine protein conjugate monoclonal antibody and anti-salbutamol protein conjugate monoclonal antibody solidified gold Standard substance, mass concentration in the prepar...

Embodiment 2

[0018]Embodiment 2: In this embodiment, in the preparation of the colloidal gold solution, the amount of trisodium citrate solution with a mass concentration of 1% is 15ml, and the colloidal gold-labeled anti-ractopamine protein conjugate monoclonal antibody or anti-albuterol protein conjugate The concentration of monoclonal antibody is 4 μg / ml; the coating concentration of rabbit anti-mouse polyclonal antibody is 0.9 mg / ml, and the coating concentration of ractopamine protein conjugate and salbutamol protein conjugate is 0.1 mg / ml. Others are with embodiment 1.

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Abstract

The invention relates to a binary detection test strip of beta-exhilarant ractopamine and salbutamol and a preparation method thereof, which can effectively solve the problem that the beta-exhilarant ractopamine and salbutamol can not be detected quickly, easily and simultaneously. The preparation method comprises the following steps of: arranging diversion glass fiber, carrier glass fiber, a nitrocellulose membrane and an absorbent cotton pulp board a on a substrate PVC (Polyvinyl Chloride) board from back to front in order; covering a coating comprising a front coating and a rear coating onthe diversion glass fiber, the carrier glass fiber and the absorbent cotton pulp board, wherein a monoclonal antibody for resisting ractopamine protein conjugates and a monoclonal antibody colloid gold marker for resisting salbutamol protein conjugates are adsorbed in the carrier glass fiber; and coating the nitrocellulose membrane with a quality control line and two test lines, wherein the quality control line is coated with rabbit antimouse multi-resistant, and the test lines are coated with ractopamine protein conjugates and salbutamol protein conjugates. The invention can be used for detecting the beta-exhilarant ractopamine and the salbutamol simultaneously, effectively, conveniently and quickly and has accurate result.

Description

1. Technical field [0001] The invention relates to a β-stimulant ractopamine and salbutamol binary detection test strip and a preparation method thereof. 2. Background technology [0002] β-agonists are a class of adrenaline mimics, named for their ability to excite (excitate) the β receptors in animal and human tissues to regulate the functions of different tissues in the body. Their chemical components are phenylethanolamine derivatives. The drug has the effect of promoting the decomposition of livestock fat and increasing muscle growth. It is used as a nutrient redistribution agent to increase lean meat percentage, reduce fat deposition and promote animal growth. It is illegally used by some livestock farmers as a breeding accelerator. Clenbuterol, Salbutamol and ractopamine are the most commonly used in the breeding industry. The use of β-stimulants in livestock feeds has caused food poisoning incidents of consumers from time to time. β-stimulants are used as feed addit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/543G01N33/531G01N21/33G01N33/52
Inventor 王玉金杨书豪刘丽杜迅胡宜亮庞向宇孙晨阳陈国参
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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