A preparation method of disodium 5'-inosinate by using crystallization process

A technology of disodium inosinate and inosinic acid, which is applied in the field of preparing disodium 5'-inosinate, can solve the problems of large amount of hydrophilic organic solvent and the like, and achieve the effect of reducing the amount of production

Active Publication Date: 2010-12-15
CJ CHEILJEDANG CORP
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Problems solved by technology

[0010] However, the problem is that when the microbial fermentation liquid is separated and concentrated with fungal cells, and then a hydrophilic organic solvent is used to obtain disodium 5'-inosinate from the concentrated solution, the amount of the hydrophilic organic solvent is large, and due to the hydrophilic With the addition of aqueous organic solvents, impurities are also extracted at the same time

Method used

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  • A preparation method of disodium 5'-inosinate by using crystallization process
  • A preparation method of disodium 5'-inosinate by using crystallization process
  • A preparation method of disodium 5'-inosinate by using crystallization process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Establishment of 5'-disodium inosinate crystallization conditions

[0029] In order to establish the optimal crystallization conditions of disodium 5'-inosinate, the solubility of disodium 5'-inosinate is determined by temperature and organic solvent. The solubility of disodium 5'-inosinate was determined by HPLC, and the concentration (% v / v) of the organic solvent in the total solution was adjusted to be 0-60% ( figure 1 ).

[0030] The supersaturation curve was obtained by measuring the solubility of disodium 5' inosinate solution in the actual fermentation broth using an online particle size analyzer; recording the temperature-based, at each organic solvent concentration (% v / v) The point where the nucleation suddenly increases and the point where the nucleation suddenly disappears when the temperature increases; the number of particles and the average size and concentration of particles are analyzed every minute. Through experiments, through the te...

Embodiment 2

[0032] Embodiment 2: realize the crystallization process of culture concentrate by the crystallization of organic solvent

[0033] Based on the results of Example 1, prepare 5'-disodium inosinate from the fermentation broth according to the method as follows:

[0034] Step 1: Cultivation of strains and isolation of bacteria

[0035] Inosinic acid-producing Corynebacteriun glutamicum CJM107 (KCCM-10227) strain is used in the present invention.

[0036] The strain was inoculated in 1L containing 46g glucose, 30g fructose, 10g yeast extract, 18gKH 2 PO 4 , 42g K 2 HPO 4 , 6g urea, 10g MgSO 4 ·7H 2O, in the fermented liquid of 30 μ g biotin and 5 mg thiamine hydrochloride, be exposed to pH 6-8, cultivate in the 18L medium of temperature 30-32 ℃ for 140 hours, until the concentration of inosinic acid in the fermented liquid reaches 5-10 %. The concentration of inosinic acid in the cultured broth was measured by HPLC. Any strains other than coryneform bacteria for producing...

Embodiment 3

[0052] Example 3: Crystallization of concentrated fermented broth by cooling crystallization

[0053] This embodiment 3 is carried out according to the same method as in steps 1 to 3 in embodiment 2. Step 4 and subsequent steps are explained below.

[0054] Step 4: Heating

[0055] Similar to Example 1, the temperature of the crystallization tube was heated to 60° C. to keep the liquid in a state where all the crystals were melted.

[0056] Step 5: Cool

[0057] As a result of the heating step, the liquid cools without crystallization. The liquid cooled at a uniform rate down to 20 °C within 5 hours, and the crystal production rate was at the level of 70% of the supernatant concentration.

[0058] Step 6: Add organic solvent and separate crystals

[0059] To extract disodium 5'-inosinate in crystalline form in the supernatant, 150 mL of 95% (v / v) aqueous methanol was added within 2 hours. During the addition of methanol, the temperature of the precipitation tube was...

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Abstract

The present invention relates to a preparation method of disodkm 5'-inosinate(IMP2Na) from 5'-inosinic acid(IMP) fermentation liquid produced by the fermentation with microorganism. By the present invention, it can provide effect to reduce waste water compared to ion exchanged resin method and to produce disodium 5'-inosinate of high purity only by the control of temperature and the use of small amount of organic solvent.

Description

technical field [0001] The present invention relates to a method for preparing 5'-inosinic acid disodium (IMP2Na) through a crystallization process, and in more detail, relates to a method for purifying 5'-inosine from the fermentation broth of inosinic acid (IMP) producing strains In the process of disodium inosinate, the method for preparing disodium 5'-inosinate is replaced by the crystallization process of the existing ion exchange resin method. [0002] That is, the present invention relates to a method for increasing the purity of disodium 5'-inosinate crystals while maintaining the same yield of disodium inosinate crystals, and at the same time, by improving the concentration of the organic solvent used in the prior art In the crystallization process, the solubility is controlled according to the characteristic temperature, the time point of crystal generation and the growth point, which greatly reduces the consumption of organic solvents. Background technique [000...

Claims

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Application Information

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IPC IPC(8): C12P19/26
CPCC12P19/40C12P19/26C12P19/28C12P19/32C12P19/34
Inventor 金日出洪淳源尹太相俞裁宪李允宰朴南燮李种勋
Owner CJ CHEILJEDANG CORP
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