Process for separating and purifying gamma-aminobutyric acid

A technology for the separation and purification of aminobutyric acid, which is applied in the field of separation and purification of γ-aminobutyric acid, and can solve the problems of GABA loss, impact on yield, and high cost of industrialization

Active Publication Date: 2010-12-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem of using this process to separate γ-aminobutyric acid is that there are many steps in the separation and purification process of the fermentation broth, and the loss of GABA is serious, such as the use of cationic resin, because its adsorption capacity for γ-aminobutyric acid is too low , affect the yield, the cost of industrialization is high, and it is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The main components of γ-aminobutyric acid conversion solution: γ-aminobutyric acid 132g / L, glutamic acid 0.15g / L, sodium acetate 12.3g / L, conversion solution volume 2.5L.

[0020] Activated carbon treatment: The powdered activated carbon is first washed with deionized water, then washed with hot deionized water, filtered, dried at 120°C, and cooled to room temperature for later use.

[0021] The transformation liquid was removed by centrifugation or filtration to remove bacteria, and the supernatant was heated at 70-80°C for 30 minutes, filtered with filter paper, and then suction-filtered with a 0.45 μm filter membrane to collect the filtrate. Adjust the collected filtrate to pH 5.0 with 2M acetic acid, add activated carbon powder according to the amount of 2%, slowly stir and decolorize at 60°C for 30min, filter through double-layer filter paper, and then use 0.45μm filter membrane to carry out suction filtration, and the filtrate Concentrate in vacuo at 55°C until i...

Embodiment 2

[0023] The main components of γ-aminobutyric acid conversion solution: γ-aminobutyric acid 144g / L, glutamic acid 0.23g / L, sodium acetate 14.3g / L, conversion solution volume 7L.

[0024] Activated carbon treatment: The powdered activated carbon is first washed with deionized water, then washed with hot deionized water, filtered, dried at 120°C, and cooled to room temperature for later use.

[0025] The transformation liquid was removed by centrifugation or filtration to remove bacteria, and the supernatant was heated at 70-80°C for 30 minutes, filtered with filter paper, and then suction-filtered with a 0.45 μm filter membrane to collect the filtrate. Adjust the collected filtrate to pH 5.0 with 2M acetic acid, add activated carbon powder according to the amount of 2%, slowly stir and decolorize at 60°C for 30min, filter through double-layer filter paper, and then use 0.45μm filter membrane to carry out suction filtration, and the filtrate Concentrate in vacuo at 55°C until it ...

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Abstract

The invention discloses a process for separating and purifying a gamma-aminobutyric acid, which aims to effectively solve the problems of complex separation and purification steps and high industrial cost which are caused by complex components of fermentation liquor in the biological preparation of the gamma-aminobutyric acid. In the process, impurities in conversion liquor are reduced by adopting a bioconversion method to simplify a separation process, and the conversion liquor is simply decolored to obtain the gamma-aminobutyric acid with high purity and recovery rate. The process has the advantages of simple method and high recovery rate of products, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of amino acid preparation, and in particular relates to a separation and purification process of gamma-aminobutyric acid. Background technique [0002] γ-Aminobutyric acid (GABA) is an important non-protein amino acid that mainly exists in the brain and spinal cord of mammals. It has various physiological functions such as lowering blood pressure, treating epilepsy, calming the nerves, and enhancing memory. It has been widely used in food and medicine. Wide range of applications. Based on the latest research results on the biological activity of GABA, the research on GABA-rich foods has become one of the current research hotspots, and many research institutions in Japan are actively developing GABA-rich functional foods. At present, the GABA used in my country mainly relies on imports. Domestic research on the separation and purification of GABA is still shallow, and the extraction process is still immature. The puri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07C229/08C07C227/40
Inventor 饶志明张术聪夏海锋刘婷婷杨套伟
Owner JIANGNAN UNIV
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