High interference resistance enzyme biofuel battery cathode and preparation method thereof
A biofuel cell and cathode technology, applied in biochemical fuel cells, battery electrodes, circuits, etc., to achieve excellent catalytic results and solve cross-interference effects
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Embodiment 1
[0025] Embodiment 1, the purification of laccase
[0026] Dissolve 50mg of laccase (Trametes Versicolor E.C.1.10.3.2, 23.76U / mg) in 500μL of 0.1M phosphate buffer solution (pH 6.0) and transfer to a dialysis bag, and soak the dialysis bag in fresh 0.1M phosphate buffer solution (pH6.0) for 4 hours with stirring and dialysis, then moved to another fresh 0.1M phosphate buffer solution (pH6.0) and stirred for 4 hours, and thus cycled for 1-2 days. Then add ammonium sulfate to the solution in the bag until it is saturated, and a precipitate precipitates out, and then the suspension is centrifuged at a speed of 5000 rpm for 10 minutes; the resulting precipitate is dissolved in about 500 μL of 0.1M phosphate buffer solution (pH6.0) , then saturated with ammonium sulfate, and precipitates were separated out; centrifuged again to obtain precipitates, and the resulting precipitates were dissolved in 300 μL 0.1M phosphate buffer solution (pH6.0) and put into dialysis tubes, and concentr...
Embodiment 2
[0027] Embodiment 2, preparation of highly anti-interference enzyme type biofuel cell cathode
[0028] 3 mg of carbon nanospheres (synthesized by the Institute of Chemistry, Chinese Academy of Sciences, 0.5 μm in diameter, reference: Macromolecular Chemistry and Physics 207(18): 1633-1639) were continuously ultrasonicated in 1 mL of acetone (the ultrasonic intensity was about 1w / cm 2 , Ultrasonic frequency is 40kHz) 20 minutes, dispersion treatment forms the suspension liquid of 3mg per milliliter, gets 2 μ L suspension liquid and is coated on the glassy carbon substrate electrode of 0.07 square centimeter, dry in air, form carbon nano material layer; After purification, 40 μL of laccase (Trametes Versicolor E.C.1.10.3.2, 23.76 U / mg) with a concentration of 1000 U / ml was mixed with 10 μL of acetone to obtain a mixture containing 40 U of laccase, and 10 μL of the above laccase mixture was coated on carbon nanomaterials layer, and dry at room temperature to obtain the enzyme lay...
Embodiment 3
[0029] Embodiment 3, preparation of highly anti-interference enzyme type biofuel cell cathode
[0030]2 mg of carbon nanofibers (purchased from Nantong Senyou Carbon Fiber Co., Ltd., with a diameter of 100 nm and a length of 50 μm) were continuously ultrasonicated in 2 mL of ethanol (ultrasonic intensity was about 1 w / cm 2 , the ultrasonic frequency is 40kHz) for 2 hours, dispersed to form a suspension of 1 mg per milliliter, 6 μL of the suspension was coated on a 0.07 square centimeter glassy carbon substrate electrode, and dried in air to form a carbon nanomaterial layer; the purified The resulting 80 μL concentration of 1000U / ml laccase (Trametes Versicolor E.C.1.10.3.2, 23.76U / mg) solution was mixed with 10 μL acetone to obtain a mixed solution containing 80 U laccase. Take 8 μL laccase mixed solution and 5% glutaraldehyde solution 2 μL was coated on the carbon nanomaterial layer, and dried at room temperature to obtain the enzyme layer; then a plastic tube was placed on t...
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