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Injectable strengthened phosphate lime/hydrogel microcapsule tissue engineering bone as well as preparation method and application thereof

A tissue-engineered bone and hydrogel technology, applied in the field of medical materials, can solve the problems of CPC’s low ability to carry cells, achieve good biocompatibility, easy operation, and reduce the difficulty of surgery

Active Publication Date: 2013-09-18
赵亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The possible reason is that the ability of CPC to carry cells is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Stir the sterilized 1.8g CPC (TTCP / DCPA=1:1) powder, 0.9g 15% Chitosan and 0.0884g PLGA electrospinning (length 3mm) for 3 minutes, mix well, and prepare CPC to Chitosan ratio 2 : 1, the CPC-Chitosan-PLGA electrospinning composite whose volume ratio of PLGA electrospinning accounts for 10% of the total volume ratio of the composite. Immediately add 0.092 g of hUCMSCs hydrogel microcapsules and CPC-Chitosan-PLGA electrospinning complex and mix slowly for 15 seconds to form CPC to Chitosan ratio of 2:1, PLGA electrospinning volume ratio and hUCMSCs hydrogel microcapsules Injectable, plastic, degradable and reinforced apatite / hydrogel microcapsule tissue engineered bone which accounts for 10% and 10% of the total volume of artificial bone respectively. Put it into a syringe for use, or stand at 37°C, solidify and shape within 30 minutes, add culture medium, put it in a cell incubator for use, culture conditions: temperature 37°C, CO 2 Concentration 90%.

Embodiment 2

[0031] Stir 1.8 g of CPC (TTCP / DCPA=1:1) powder, 0.9 g of 15% Chitosan and 0.0884 g of PLGA electrospinning (3mm) that have been sterilized for 3 minutes, mix well, and prepare a ratio of CPC to Chitosan of 2: 1. The CPC-Chitosan-PLGA electrospinning composite whose volume ratio of PLGA electrospinning accounts for 10% of the total volume of the composite. Immediately add 0.184 g of hUCMSCs hydrogel microcapsules and CPC-Chitosan-PLGA electrospinning complex and mix slowly for 15 seconds to form CPC to Chitosan ratio of 2:1, PLGA electrospinning volume ratio and hUCMSCs hydrogel microcapsules Injectable, plastic and degradable reinforced apatite / hydrogel microcapsule tissue engineered bone which accounts for 10% and 20% of the total volume of artificial bone respectively. Put it into a syringe for use, or stand at 37°C, solidify and shape within 30 minutes, add culture medium, put it in a cell incubator for use, culture conditions: temperature 37°C, CO 2 Concentration 90%.

Embodiment 3

[0033] Stir 1.8 g of CPC (TTCP / DCPA=1:1) powder, 0.9 g of 15% Chitosan and 0.0884 g of PLGA electrospinning (3mm) that have been sterilized for 3 minutes, mix well, and prepare a ratio of CPC to Chitosan of 2: 1. The CPC-Chitosan-PLGA electrospinning composite whose volume ratio of PLGA electrospinning accounts for 10% of the total volume of the composite. Immediately add 0.276 g of hUCMSCs hydrogel microcapsules and CPC-Chitosan-PLGA electrospinning complex and mix slowly for 15 seconds to form CPC to Chitosan ratio 2:1, PLGA electrospinning volume ratio and hUCMSCs hydrogel microcapsules Injectable, plastic and degradable reinforced apatite / hydrogel microcapsule tissue engineered bone which account for 10% and 30% of the total volume of artificial bone respectively. Put it into a syringe for use, or stand at 37°C, solidify and shape within 30 minutes, add culture medium, put it in a cell incubator for use, culture conditions: temperature 37°C, CO 2 Concentration 90%.

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Abstract

The invention discloses an injectable strengthened phosphate lime / hydrogel microcapsule tissue engineering bone which is prepared from the following components in percentage by weight: 20-75% of calcium phosphate cement, 20-75% of chitosan, 1-10% of PLGA electric spin and 3-155 of hUCMSCs hydrogel microcapsule. The invention also discloses a preparation method of the tissue engineering bone and application of the tissue engineering bone as a bone defect repairing material. The tissue engineering bone not only maintains original good biocompatibility and osteogenesis inducing property of phosphate lime, but also obviously improved mechanical properties and capability for carrying seed cells. The tissue engineering bone can be used for treating bone defect or bone deficiency caused by various diseases and has the characteristics of easy operation, convenient molding, maximum wound reduction, operation difficulty reduction, patient pain alleviation, less infection danger, less scar formation and the like.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a novel bone biorepair composite material for constructing injectable and in-situ solidified phospholime / hydrogel microcapsule tissue engineered bone through tissue engineering methods and its application. Background technique [0002] Bone defects caused by congenital, trauma, infection, tumor and other reasons are common clinical problems. At present, autologous bone grafting, allografting bone grafting and artificial synthetic bone filling materials are used to fill and repair bone defects, but there are still many deficiencies. Autologous bone transplantation: Pain at the donor site, blood loss, infection, structural damage, functional impact, limited number of bone harvested, allogeneic bone: immune rejection, disease infection, artificial synthetic bone filling materials can only fill and support bone defects. , weak osteoinductive ability. With the development of economi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/18A61L27/12A61L27/52D01D5/00C08J3/075
Inventor 赵亮
Owner 赵亮
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