Strain for degrading wool scale layer

A flake layer and wool technology, applied in the field of Pseudomonas, can solve the problems of increased protease process steps, time-consuming, increased cost, etc., and achieve the effects of avoiding environmental impact, mild working conditions, and good anti-felt performance.

Inactive Publication Date: 2012-02-01
DONGHUA UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

In order to solve this problem, people are studying the third step, using the resin method or TG enzyme (transglutaminase), which increases the protease process steps, prolongs the time consumption, and increases the cost. not on the market

Method used

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  • Strain for degrading wool scale layer
  • Strain for degrading wool scale layer
  • Strain for degrading wool scale layer

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Experimental program
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Effect test

Embodiment 1

[0027] The new strain Pseudomonas sp. 3096-4 (Pseudomonas sp.3096-4) has been deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No.3888.

[0028] The source of the strain: the strain is derived from the rotten wool of the sheep farm in Songjiang, Shanghai. The specific isolation method is as follows:

[0029] (1) Enrichment Take 1g of rotten wool from the piled wool and place it in enrichment medium (0.5g of beef extract, 1g of peptone, 0.5g of sodium chloride, pH7.0-7.6), and culture at 28°C and 120 rpm 48h; then take 2mL of its culture solution and transfer it to enrichment medium 2, and further enrich and cultivate for 48h at 28°C and 120 rpm;

[0030] (2) Primary screening Take 100 μL of the final enrichment solution and spread it on the phosphoric acid digestion protein medium plate, and screen out the growing single colonies;

[0031] (3) Re-screening The bacterial strains obtaine...

Embodiment 2

[0057] In order to further enhance the industrial applicability of the protease liquid produced by the fermentation of Pseudomonas 3096-4, the present invention salts out and precipitates the fermented liquid, and after drying, dissolves it in a buffer solution to measure its anti-pilling effect. The specific operation method is to add 30% ammonium sulfate to the fermented enzyme solution of the monas 3096-4, place it at 4°C for 5-12 hours, centrifuge, remove the supernatant, freeze-dry, and set aside. When using, first use buffer (phosphate buffer or Tris-HCl buffer, pH 7.4-7.8), and dissolve to 0.5% (w / v). To treat the woolen cloth, the bath ratio is 1:30, act at 30°C for 50 minutes, wash and dry after treatment, and the felt shrinkage rate is 4.2%.

[0058]

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PUM

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Abstract

The invention relates to pseudomonas sp. 3096-4 for degrading a wool scale layer, wherein the preservation number of the pseudomonas sp. is CGMCC (China General Microbiological Culture Collection Center) No.3888, and the 16S rRNA sequence thereof is shown as the table 1. Protease produced by the stain has the advantages of high activity, good felting resistance to wool textiles, moderate action condition and good stability. The stain can be used for independently degrading the wool scale layer without pretreatment steps, cannot cause the reduction of the strength of the wool textiles and can be used for replacing the traditional chlorination-resin method in the wool spinning industry so as to avoid the generation of organic chloride and influence on the environment and the human body health.

Description

technical field [0001] The invention belongs to the field of pseudomonas, in particular to a bacterial strain that degrades wool scale layer. Background technique [0002] Wool fabric is the second largest natural fiber fabric except cotton fabric. Its inherent lightness, wear resistance, moisture absorption and non-flammability make wool fabric a high-grade textile. It has advantages that other fiber fabrics cannot match, so it is popular among people. A welcome premium product. As one of the largest wool producing countries, my country has an annual wool output of about 300,000 tons. However, the existence of the scale layer of wool raw wool seriously affects the quality of wool, such as hand feeling, washing felt shrinkage, dyeing performance, itching and so on. It can be said that the wool scale layer is the "culprit" of many disadvantages of wool as a high-quality textile. [0003] In order to develop high-quality woolen textiles, people have long studied the use of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/38D01C3/00C12N1/20
Inventor 曹张军黄铮华蔡少博张兴群周美华洪枫
Owner DONGHUA UNIV
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