High-efficient restaurant-kitchen waste degrading microorganism bacterial agent and preparation method and application thereof
A technology of composite microbial agent and kitchen waste, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of large odor, undiscovered, poor effect, etc.
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Embodiment 1
[0020] This example is the preparation of compound microbial bacterial agent.
[0021] (1) Preparation of composite bacteria
[0022] 1) Cultivation of protein degrading bacteria
[0023] The protein-degrading bacteria used in this example is the strain numbered HJK6.2-2 preserved by the Guangdong Provincial Microorganism Preservation and Management Center. It was transferred from the slant medium to a 10ml liquid test tube for shaking culture, and gradually enlarged to a 3L fermenter. Cultivate in functional medium, culture temperature is 30-37°C, culture time is 12-24h, the best is 16-18h. Then the cells were collected by centrifugation.
[0024] The functional medium used was soluble starch 20g, potassium nitrate 1g, sodium chloride 0.5g, K 2 HPO 4 ·3H 2 O0.5g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O0.01g, 20g agar, 1000ml water, pH7.2~7.4. When preparing, first use cold water to make the starch into a paste, pour it into boiling water, heat it on the fire, add other i...
Embodiment 2
[0043] This example is the preparation of compound microbial bacterial agent.
[0044] (1) Preparation of composite bacteria
[0045] According to the culture method of Example 1, each strain was cultured separately, and then centrifuged to collect the thalline, and mixed according to the following ratio to prepare a composite thallus, protein degrading bacteria: starch degrading bacteria: sugar degrading bacteria: fat degrading bacteria: cellulose degrading bacteria =1.5:1:1.5:1.5:1.5.
[0046] (2) Preparation of carrier materials
[0047] Cinder, zeolite, wood shavings, buckwheat husk, bran, and activated carbon are mixed in the following parts by mass to make carrier material, cinder: zeolite: wood shavings: buckwheat husk: bran: activated carbon=1: 1: 0.5: 0.5: 0.5: 0.05
[0048] (3) Preparation of composite microbial bacterial agent
[0049]The composite microbial bacterial agent is prepared by mixing the composite bacterium with the carrier material, and the composit...
Embodiment 3
[0051] This example is the test of the degradation rate of kitchen waste with the microbial inoculant (powder) prepared in Example 1.
[0052] 1. Composition of kitchen waste:
[0053] For the remaining meals in the cafeteria, remove large and hard parts such as bones.
[0054] 2. Put the composite microbial bacterial agent prepared in Example 1 into a garbage machine, continue to ferment for 24 hours, and then add 1kg of garbage. The mixing ratio of kitchen waste and microbial agent is about 1:6.0.
[0055] 3. Determination of garbage degradation rate:
[0056] Weigh the weight of the garbage machine and the bacterial agent when no garbage is added, and it is 21.07kg. After adding 1kg of food waste every other day, the total weight of the garbage machine, the bacterial agent and the remaining garbage is weighed 10 days later to be 23.15kg. According to the formula of weight average reduction efficiency
[0057]
[0058] B=21.07+1×10=31.07, C=23.15, A=1×10=10
[0059] ...
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