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Eosinophilic kidney type avian infectious bronchitis virus (IBV) virulent vaccine strain and application thereof

A chicken infectious and bronchitis technology, applied in antiviral agents, viruses/bacteriophages, medical preparations containing active ingredients, etc., to achieve good immunogenicity

Inactive Publication Date: 2011-01-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that after immunizing chickens with different nephrophilic infectious bronchitis live vaccines in my country, they were challenged with the popular nephrophilic infectious bronchitis virus, but none of them were effective against the current popular nephrophilic infectious bronchitis virus. Provides effective protection against attacks by wild strains

Method used

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  • Eosinophilic kidney type avian infectious bronchitis virus (IBV) virulent vaccine strain and application thereof
  • Eosinophilic kidney type avian infectious bronchitis virus (IBV) virulent vaccine strain and application thereof
  • Eosinophilic kidney type avian infectious bronchitis virus (IBV) virulent vaccine strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition and characteristic determination of embodiment 1 virus

[0023] In this example, the suspected nephrophilic chicken infectious bronchitis disease material isolated from the diseased chicken flock in Jiangsu, my country, was inoculated with specific pathogen free (Specific Pathogen Free, SPF) chicken embryos to isolate the virus, and the virus strain was tested in SPF. The nephrophilic chicken infectious bronchitis virulent vaccine strain was obtained by cloning and purifying the virus by limiting dilution three times on chicken embryos, named Ck / Jiangsu / DS10 / 2008, DS10 for short. The virus DS10 was returned to inoculate SPF chickens, and the pathogenicity and immunogenicity of the virus were evaluated.

[0024] specific method:

[0025] (1), virus DS10 amplification: virus DS10 is diluted 10 with sterile PBS buffer solution 3 After doubling, 11-day-old SPF chicken embryos were inoculated through the allantoic cavity at an inoculation volume of 0.2 mL / em...

Embodiment 2

[0030] Embodiment 2 chicken embryo neutralization protection test

[0031] In order to verify the cross protection of virus DS10 to respiratory infectious bronchitis virus, hyperimmune serum was prepared respectively for virus DS10 and virus M41 (respiratory infectious bronchitis virus), and the hyperimmune serum was diluted with virus DS10 and virus DS10 respectively. Virus M41 was tested for cross-protection.

[0032] (1), preparation of virus and serum: virus DS10 and virus M41 (purchased from the China Veterinary Drug Control Institute, referred to as "China Supervision Institute") were diluted with sterile PBS buffer for 10 3 After doubling, the 9-11-day-old SPF chicken embryos were inoculated through the allantoic cavity. Within 36-48 hours after inoculation, healthy and alive chicken embryos were frozen in a refrigerator at 2-8°C for 6-8 hours, and the virus allantoic fluid was aseptically collected, subpackaged, and placed in a refrigerator at -70°C. Among them, viru...

Embodiment 3

[0040] Embodiment 3 immunogenicity test

[0041] Use the virus DS10 or M41 as the virus strain to prepare the oil emulsion inactivated vaccine respectively, and carry out the cross-challenge protection test.

[0042] (1), virus and vaccine preparation: the preparation of virus allantoic fluid sees embodiment 1, and allantoic fluid detects EID 50 Valence greater than or equal to 10 7.0 EID 50 / mL, inactivated by formaldehyde, and then mixed with white oil adjuvant, the oil emulsion inactivated vaccines of virus DS10 and M41 were prepared respectively according to conventional methods.

[0043] (2) SPF chicken: SPF chicken embryos were purchased from Jinan Saisi Poultry Technology Co., Ltd. of Shandong Poultry Institute, and were hatched by themselves until hatched, and then raised in isolators.

[0044] (3), grouping and immunization of experimental animals

[0045] The 14-day-old SPF chickens were divided into groups, and subcutaneously immunized with 0.2 mL of the corresp...

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Abstract

The invention relates to an eosinophilic kidney type avian infectious bronchitis virus (IBV) vaccine strain, in particular to an eosinophilic kidney type IBV virulent vaccine strain and application thereof, belonging to the technical field of organisms. The eosinophilic kidney type avian IBV virulent vaccine strain has CGMCC (China General Microbiological Culture Collection) No. 3957, and fiber axon protein of the eosinophilic kidney type avian IBV virulent vaccine strain has a gene sequence shown as SEQ ID NO:1. The invention provides a vaccine composition which comprises the eosinophilic kidney type avian IBV virulent vaccine strain and pharmaceutically acceptable adjuvants or carriers. The eosinophilic kidney type IBV virulent vaccine strain provided by the invention has favorable immunogenicity to the current epidemic eosinophilic kidney type IBV. A vaccine prepared by a DS10 virus not only can effectively resist the attack of self virulent strains, but also can effectively prevent the attack of a respiratory vaccine strain, therefore, the avian IBV can be effectively prevented and treated.

Description

technical field [0001] The invention relates to a nephrophilic chicken infectious bronchitis virus vaccine strain, in particular to a nephrophilic type infectious bronchitis virus virulent vaccine strain and application thereof, belonging to the field of biotechnology. Background technique [0002] Chicken infectious bronchitis (Infectious Bronchitis, IB) is an acute, highly contagious infectious disease of chickens caused by infectious bronchitis virus (IBV). According to the pathogenic symptoms after virus infection, that is, the pathogenic type, it can be divided into nephrophilic type and respiratory type. Nephrophilic infectious bronchitis is mainly characterized by kidney lesions, and genitourinary system symptoms, accompanied by respiratory symptoms, manifested as pale and enlarged kidneys, dilated renal tubules and ureters filled with urate, and discharge of white loose stools. Chickens of various ages and genders are susceptible to infection, but it mainly affects ...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
Inventor 唐应华吴培培于漾张道华揭鸿英侯继波何家惠
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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