Bacillus stain for preventing and controlling rice blast and rice sheath blight

A technology for rice blast and bacillus, applied in the direction of bacteria, fungicides, and microbial-based methods, can solve the problems of polluted environment and increased drug resistance of pathogenic bacteria

Inactive Publication Date: 2011-02-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since tricyclazoles are chemically synthesized drugs, although their application effect is good in the short term, if similar chemical agents are used continuously on a large scale for a long time, it will seriously pollute the environment and lead to a substantial increase in the resistance and drug resistance of pathogenic bacteria.

Method used

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  • Bacillus stain for preventing and controlling rice blast and rice sheath blight
  • Bacillus stain for preventing and controlling rice blast and rice sheath blight
  • Bacillus stain for preventing and controlling rice blast and rice sheath blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening method for bacterial strain XZJ.BP01

[0021] 70% ethanol was used to disinfect the sweet-scented osmanthus fruit, and washed with sterilized water for 3 times. Then the fruit is crushed and centrifuged to obtain the supernatant, which is applied to the PDA solid medium (200 grams of potatoes, 15 grams of sucrose, 18 grams of agar, 1000 milliliters of water, pH7.0), inoculated with rice blast and sheath blight pathogen, placed Cultured in a constant temperature incubator at 28°C. Screen out strains that inhibit the growth of pathogenic bacteria. Monoclonal strains were isolated by streak culture method.

Embodiment 2

[0022] Embodiment 2 Antibiotic research of antagonistic bacteria

[0023] Rice sheath blight bacteria and bacterial strain XZJ.BP01 were inoculated simultaneously in a plate equipped with PDA medium, and the diameter of the inoculated bacteria cake was 1 cm. The distance between the two mushroom cakes is 5 cm. After inoculation, they were cultured in a constant temperature incubator at 28°C, and the colony morphology was observed after 6 days.

[0024] The sheath blight bacteria and the strain XZJ.BP01 were inoculated by the confrontation method, and the sheath blight mycelia and normal mycelia on the edge of the confrontation were picked and observed under an ordinary optical microscope and scanned by an electron microscope.

Embodiment 3

[0025] The identification of embodiment 3 bacterial strains

[0026] A. Morphological characteristics: After culturing on PDA medium at 28° C. for 3 days, routine bacterium morphology was observed with a scanning electron microscope and an optical microscope.

[0027] B. Physiological and biochemical experiments: Physiological and biochemical identification of bacterial strains was carried out with reference to the contents of "Bergey's Bacterial Identification Manual (Eighth Edition)".

[0028] Bacterial universal primers P2F (5'-agccaggatcaaactctg-3') and P5R (5'-aggaggtgatccagccgca-3') were used to amplify and sequence the 16rDNA of strain XZJ.BP01, and the obtained 16rDNA was compared on NCBI. The similarity of Bacillus reached 99%, and it was basically determined to be Bacillus pumilus.

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Abstract

The invention discloses Bacillus pumilux XZJ.BP01 having antagonistic effect on rice sheath blight and rice blast pathogenic bacteria, which is separated from osmanthus fruit. The strain was collected in China Center for Type Culture Collection on October 27th, 2009, with a collection number of CCTCC No.M209235. The strain XZJ.BP01 has antagonistic effect on the rice blast and rice sheath blight pathogenic bacteria, and the biological metabolic products of the strain has a sheath blight bacterium inhibition rate of over 99 percent; meanwhile, in field test of rice blast, the infection index of the rice applied with the fermentation solution of the strain is 16.7 percent, while the infection index of reference rice is up to 67.8 percent. As a result, the strain of the invention has a great significance for solving rice blast and rice sheath blight and has a remarkable effect.

Description

technical field [0001] The present invention relates to a bacterial strain of the genus Bacillus, in particular to a bacterial strain of the genus Bacillus pumilus isolated from sweet-scented osmanthus fruit and having a strong antagonistic effect on rice blast and sheath blight pathogens XZJ.BP01 , the secondary metabolites produced by the strain in PDA medium and cultured at 30°C also have strong antagonistic effects on rice sheath blight and rice blast. Background technique [0002] Rice is one of the important food crops. However, with the rapid development of economy, the gradual increase of population and the sharp decrease of arable land, coupled with the attacks of diseases and insect pests, rice production is facing increasing pressure. Rice blast (Pyricularia) and sheath blight (Thanatephorus cucumeris) are the two most harmful diseases in my country's rice fields. Rice blast is widely distributed and occurs in more than 80 countries and regions in the world. Gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01P3/00C12R1/07C12N1/20
Inventor 徐正君姜凯李利华高晓玲高鸿张小平马炳田徐永菊山口五十麿朱建清邓晓建钟凯王平荣黄静
Owner SICHUAN AGRI UNIV
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