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Composition for inhibiting aging containing extracts from plant buds

A skin aging and extract technology, which is applied in skin care preparations, cosmetic preparations, cosmetics, etc., can solve the problems of reduced activity, reduced number of epidermal stem cells, hindered production of collagen fibers and elastic fibers, etc., to achieve enhanced activity, Improve antioxidant capacity and promote value-added effects

Active Publication Date: 2012-11-14
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is known that wrinkles are formed centering on the above-mentioned disappearing part, and the healing ability of the wound is reduced
Of course, it is not because the number or activity of epidermal stem cells is reduced so that they cannot perform the functions of the skin that the human body has, but from the perspective of cosmetology, it can cause problems such as anxiety
Here, it is known that epidermal cells also affect fibroblasts, which are dermal cells existing thereunder, and promote collagen production (E1. Ghalbzouri, et al., J Invest Dermatol. 124:79-86 (2005)), But the missing epidermal stem cells hinder the production of collagen fibers and elastic fibers in the dermis

Method used

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  • Composition for inhibiting aging containing extracts from plant buds
  • Composition for inhibiting aging containing extracts from plant buds
  • Composition for inhibiting aging containing extracts from plant buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] [Example 1] Skin external preparation composition containing sprout extract as an active ingredient

[0048] 1-1. Preparation of Sprout Extract

[0049] This embodiment relates to effective elution of the active ingredients of the sprouts or sprouted plants of redwood, bilberry, peach, cabbage, cauliflower, sunflower, and kale.

[0050] First, new shoots are harvested from redwood, bilberry, and peach trees from spring to summer. In order to prevent browning caused by enzyme reactions, they are frozen and stored between minus 20°C and minus 30°C.

[0051] Next, in the case of cabbage, cauliflower, sunflower, and kale, the selected seeds are immersed in water for about 6 to 10 hours, then cotton is spread in the culture container, and the seeds are evenly sprinkled without overlapping, before germinating Water is supplied in a way that prevents the cotton from drying out. Cover the seeds with a black cloth until the sprouts are about 2 / 3, and then place them in a sunny place. ...

experiment example 1

[0061] [Experimental example 1] Observation of increased activity of skin epidermal stem cells

[0062] In a 24-well plate incubator with 2×10 5 After injecting the keratinocytes isolated from human epidermis at a concentration of one, they were cultured in DMEM medium injected with 2% fetal bovine serum. After that, it was replaced with a medium containing the sprout extract prepared in Example 1 at a concentration of 50 and 500 ppm, respectively, and cultured for 48 hours. The negative control group was a group in which no sprout extract was injected into the above medium, and a group obtained by injecting 10% fetal bovine serum instead of 2% fetal bovine serum into the above medium was used as a positive control group. Remove the supernatant, wash twice with phosphate buffer solution, add 50ul of cell lysis buffer (Cell lysis buffer; 20mM Tris-HCl (pH7.4), 250mM NaCl, 1% Triton X-100), and let it stand for 30 minute. After washing twice with a phosphate buffer solution, a ce...

experiment example 2

[0068] [Experimental Example 2] Observation of the increase in skin cell proliferation by the new bud extract

[0069] 2-1. Proliferation of keratinocytes

[0070] In a 96-well plate incubator, keratinocytes isolated from human epidermis were added at a concentration of 5000 per well, and cultured with KGM (keratinocyte growth medium, Lonza) medium for 24 hours. After that, it was replaced with a KBM (keratinocyte basal medium) medium containing the sprout extract prepared in Example 1 at a concentration of 50 and 500 ppm, respectively, and cultured for 48 hours. After injecting the WST-1 solution (Roche) at 1 / 10 times, the reaction was carried out in an incubator at 37°C for 2 hours. After the completion of the culture, the degree of color development at 450 nm was measured on the plate incubator to quantify the degree of cell proliferation. The results are shown in Table 3 below.

[0071] table 3

[0072]

[0073] Referring to Table 3 above, it can be seen that the proliferatio...

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Abstract

The present invention relates to a composition containing extracts of plant sprouts including sequoia sprouts, bilberry sprouts, peach tree sprouts, Korean cabbage sprouts, broccoli sprouts, sunflower sprouts and kale sprouts as active ingredients. The composition of the present invention promotes skin cell growth and survival, and the growth of skin stem cells, thereby inhibiting skin aging and improving the appearance of the skin.

Description

Technical field [0001] The present invention relates to a composition for inhibiting skin aging containing plant sprout extracts, and in particular, to a composition containing sprouts from redwood sprouts, bilberry sprouts, peach sprouts, sprouted cabbage, sprouted cauliflower, sprouted sunflower and sprouted kale The sprout extract is used as an effective ingredient to inhibit skin aging. Background technique [0002] A representative cell of adult stem cells present in the skin is epidermal stem cells. The epidermal stem cells located at the boundary between the epidermis and the dermis of the skin divide into two, one remains as epidermal stem cells, and the other continues to divide and differentiate into epidermal cells. Therefore, in terms of green embryology, the boundary between the epidermis and the dermis held in the adult body became flat due to intrinsic aging and photoaging, and its area was reduced. As a result, part of epidermal stem cells was observed to disappe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/97A61Q19/08
CPCB05C5/02B05C17/00516B05C21/00
Inventor 张希景闵大珍朴元锡郑智彗朴録贤崔贤贞金知贤金志映
Owner AMOREPACIFIC CORP
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