Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for natural product podophyllotoxin

A technology of podophyllotoxin and natural products, applied in the production of bulk chemicals, organic chemistry, etc., can solve the problems of serious pollution and large amount of organic reagents, and achieve the effects of less pollution, fast processing speed, and easy storage and recovery

Inactive Publication Date: 2011-02-09
NANJING ZELANG AGRI DEV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Podophyllotoxin belongs to the ring lignin lactone in the lignin class. Most of the existing extraction methods are traditional techniques. The amount of organic reagents is large and the pollution is serious. The process includes: extraction with ethanol or methanol, extraction after concentration, crystallization or column chromatography

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take Taoerqi raw material, select and remove impurities and dry, crush 60 mesh, weigh 2kg, put it into the extraction kettle, seal it, adjust the extraction temperature to 35°C, the temperature of the separation tower to 45°C, the extraction pressure to 41MPa, and the analytical pressure to 8MPa , CO 2 The gas is released from the steel cylinder, liquefied in the liquefaction tank after passing through the gas purifier, enters the storage tank and 1% chloroform entrainer is sent into the purifier with a high-pressure metering pump, and reaches the specified temperature and pressure through the preheater at a flow rate of 35kg / h , open the pressure regulating valve, control the above parameters through the pressure regulating valve, and dynamically extract for 2 hours. Release the extract after shutting down, collect the extract and wash it with 3 times the amount of deionized water backwashing for 3 times, then dissolve the extract with chloroform, add filtration and pu...

Embodiment 2

[0030] Take Taoerqi raw material, select and remove impurities and dry, crush 40 meshes, weigh 2kg, put it into the extraction kettle, seal it, adjust the extraction temperature to 40°C, the temperature of the separation tower to 50°C, the extraction pressure to 43MPa, and the analytical pressure to 10MPa , CO 2 The gas is released from the steel cylinder, liquefied in the liquefaction tank after passing through the gas purifier, enters the storage tank and 2% chloroform entrainer is sent into the purifier with a high-pressure metering pump, and reaches the specified temperature and pressure through the preheater at a flow rate of 28kg / h , open the pressure regulating valve, control the above parameters through the pressure regulating valve, and extract dynamically for 3 hours. Release the extract after shutting down, collect the extract and wash twice with 6 times the amount of deionized water, then dissolve the extract with chloroform, filter and pump it into a medium-pressu...

Embodiment 3

[0032] Take Taoerqi raw material, select and remove impurities and dry, crush 60 mesh, weigh 2kg, put it into the extraction kettle, seal it, adjust the extraction temperature to 45°C, the temperature of the separation tower to 4°C, the extraction pressure to 42MPa, and the analytical pressure to 10MPa , CO 2 The gas is released from the steel cylinder, liquefied in the liquefaction tank after passing through the gas purifier, enters the storage tank and 1% chloroform entrainer is sent into the purifier with a high-pressure metering pump, and reaches the specified temperature and pressure through the preheater at a flow rate of 32kg / h , open the pressure regulating valve, control the above parameters through the pressure regulating valve, and extract dynamically for 3 hours. After shutting down, release the extract, collect the extract and wash it with 5 times the amount of deionized water backwashing for 3 times, then dissolve the extract with chloroform, add and filter to a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method for natural product podophyllotoxin, which comprises the following steps of pulverizing raw materials into 20 to 60 meshes; taking CO2 and chloroform as solvents; super-critically extracting for 1 to 3 hours; washing analytic matters by utilizing deionized water; dissolving by utilizing chloroform; carrying out a medium-pressure alumina column chromatography; carrying out gradient elution by utilizing a petroleum ether-methanol chloroform mixing solution; collecting high-concentration fraction, concentrating and crystallizing; and recrystallizing the methanol chloroform mixing solution. The method for producing the podophyllotoxin has the advantages of less pollution, high extraction efficiency and less usage of organic solvents.

Description

Technical field: [0001] The present invention relates to a kind of preparation method of natural product podophyllotoxin, especially one adopts supercritical CO 2 Extraction Techniques Extraction and Preparation of Podophyllotoxin by Medium Pressure Alumina Column Chromatography. Background technique: [0002] The principle of supercritical carbon dioxide extraction and separation process is to use supercritical carbon dioxide to have a special dissolving effect on some special natural products, and to use the relationship between the dissolving capacity of supercritical carbon dioxide and its density, that is, to use the influence of pressure and the dissolving capacity of supercritical carbon dioxide. of. In the supercritical state, the supercritical carbon dioxide is contacted with the substance to be separated, so that it selectively extracts the components of polarity, boiling point and molecular weight in sequence. Of course, the extract corresponding to each pressur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D493/04
CPCY02P20/54
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com